[1]闫志鹏 吴杰闫曙光 李京涛 魏海梁 常占杰 纪泛扑.基于数据挖掘与网络药理学探讨新型冠状病毒肺炎恢复期中医药用药规律及机制[J].陕西中医药大学学报,2023,(05):029-38.[doi:10.13424/j.cnki.jsctcm.2023.05.006]
　YAN Zhipeng WU Jie YAN Shuguang LI JingtaoWEI Hailiang CHANG Zhanjie JI Fanpu.Study on the Law and Mechanism of Traditional Chinese Medicine in the Recovery Period of COVID-19 Based on Data Mining and Network Pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2023,(05):029-38.[doi:10.13424/j.cnki.jsctcm.2023.05.006]
点击复制

基于数据挖掘与网络药理学探讨新型冠状病毒肺炎恢复期中医药用药规律及机制
分享到：

《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2023年05期
页码:
029-38
栏目:
疫病防治
出版日期:
2023-10-14

文章信息/Info

Title:
Study on the Law and Mechanism of Traditional Chinese Medicine in the Recovery Period of COVID-19 Based on Data Mining and Network Pharmacology
文章编号:
2096-1340(2023)05-0029-10
作者:
闫志鹏1 吴杰2闫曙光2 李京涛2 魏海梁2 常占杰2 纪泛扑3
1.陕西中医药大学第一临床医学院，陕西 咸阳 712000;
2.陕西中医药大学附属医院， 陕西 咸阳 712000;
3.西安交通大学第二附属医院，陕西 西安 710004
Author(s):
YAN Zhipeng1 WU Jie2 YAN Shuguang2 LI Jingtao2WEI Hailiang2 CHANG Zhanjie2 JI Fanpu3
1.First Clinical School of Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712000,China;
2.Affiliated Hospital of Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712000,China;
3.Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)
关键词:
关键词：数据挖掘; 网络药理学; 新型冠状病毒肺炎; 恢复期; 中医药用药规律
Keywords:
Key words： Data mining; Network pharmacology; COVID-19 Recovery period; Medication rule of Traditional Chinese Medicine
分类号:
R285
DOI:
10.13424/j.cnki.jsctcm.2023.05.006
文献标志码:
A
摘要:
摘 要：目的 基于数据挖掘分析新型冠状病毒肺炎（Corona virus disease 2019,COVID19）恢复期临床研究、名医经验及官方公布恢复期方药组方规律，应用网络药理学研究核心药物及其化合物在COVID19恢复期的作用机制，为新冠肺炎恢复期的防治提供指导。方法 通过检索中国知网等数据库以构建新冠肺炎恢复期中医处方数据库，采用数据挖掘和关联规则分析等方法，研究新冠肺炎恢复期方药组方规律，筛选核心药组；进一步采用网络药理学方法，通过GO分析和KEGG通路富集分析，研究核心药物的生物信号通路，阐明其作用机制。结果 筛选官方公布的新冠肺炎恢复期处方86套，发现13味高频中药，进一步关联分析得到14个高频药组，结合复杂网络分析及聚类分析确定新冠肺炎恢复期核心药组。采用网络药理学方法，研究核心药组作用机制，筛选得到疾病核心作用靶点与主要化合物成分靶点交集34个，GO富集分析367条，KEGG分析108条。结论 新冠肺炎恢复期用药规律以补脾益肺，益气养阴为主，祛湿利痰、解毒化瘀为辅，病机以正虚邪恋为特点，“茯苓半夏党参陈皮”为新冠肺炎恢复期处方中的核心药组。网络药理学分析进一步分析发现核心药组的关键成分木犀草素、黄芩素、柚皮素等可调控LI6、TNF、AKT等关键靶点发挥作用，松弛素信号通路、糖尿病并发症AGERAG等信号通路可能为其关键通路。
Abstract:
Abstract： Objective Based on data mining,to analyze the clinical research,famous doctors experience and the officially announced prescription rules of COVID-19 in the recovery period,and to apply network pharmacology to study the mechanism of core drugs and their compounds in the recovery period of COVID-19,so as to provide guidance for the prevention and treatment of COVID-19 in the recovery period.Methods The Chinese medicine prescription database in the recovery period of COVID-19 was constructed by searching CNKI and other databases.The data mining and association rule analysis methods were used to study the rules of prescriptions in the recovery period of COVID-19 and screen the core drug groups;Further adopting network pharmacology methods,GO analysis and KEGG pathway enrichment analysis were used to study the biological signaling pathways of core drugs and elucidate their mechanisms of action.Results 86 sets of official prescriptions for COVID-19 convalescence were screened,13 highfrequency Chinese drugs were found,14 highfrequency drug groups were further obtained by association analysis,and core drug groups for COVID-19 convalescence were determined by complex network analysis and cluster analysis.Using network pharmacology methods,the mechanism of action of core drug groups was studied,and 34 action targets and main compound components were screened.367 GO enrichment analyses and 108 KEGG analyses were conducted.Conclusion In the convalescent period of COVID19,the drug use pattern is mainly to invigorate the spleen and lungs,replenish Qi and nourish Yin,supplemented by removing dampness and phlegm,detoxification and removing blood stasis.The pathogenesis is characterized by positive deficiency and evil love.“Poria-Pinellia ternata-Codonopsis pilosula-Chenpi” is the core drug group in the convalescent prescription of COVID-19.Through further analysis of network pharmacology,it is found that the key components of the core drug group,such as luteolin,baicalein,naringenin,could regulate key targets such as LI6,TNF,AKT to play a role,and relaxin signaling pathway,AGERAG and other signaling pathways of diabetes complications might be its key pathways.

参考文献/References:

［1］Lotfi M,Hamblin MR,Rezaei N.COVID-19:Transmission,prevention,and potential therapeutic opportunities［J］.Clinica chimica acta;international journal of clinical chemistry，2020,508:254-266.
［2］向锋,周宜,廖小娟，等.基于UHPLC-Q-Exactive Orbitrap MS的湖南新冠2号方的化学成分研究［J］.湖南中医药大学学报,2021,41(9):1313-1320.
［3］国家药典委员会.中华人民共和国药典：一部［M］.北京：中国医药科技出版社,2020.
［4］Wiersinga WJ,Rhodes A,Cheng AC,et al.Pathophysiology,Transmission,Diagnosis,and Treatment of Coronavirus Disease 2019 (COVID-19):A Review［J］.JAMA,2020,324(8):782-793.
［5］Guan WJ,Ni ZY,Hu Y,et al.Clinical characteristics of coronavirus disease 2019 in China［J］.The New England Journal of Medicine,2020,382(18):1708-1720.
［6］Huang C,Huang L,Wang Y,et al.6-month consequences of COVID-19 in patients discharged from hospital:a cohort study［J］.Lancet,2021,397(10270):220-232.
［7］Xiong Q,Xu M,Li J,et al.Clinical sequelae of COVID-19 survivors in Wuhan,China:a single-centre longitudinal study［J］.Clinical Microbiology and Infection，2021,27(1):89-95.
［8］王珏，王彬杰，杨加彩，等.新型冠状病毒肺炎诱发肺纤维化的机制及相关治疗研究进展［J］.中华烧伤杂志,2020,36(8):691-697.
［9］孙玉洁,曾兰,刘林,等.基于新型冠状病毒肺炎的因、机、证探讨湿毒疫的发生发展规律［J］.中华中医药杂志,2021,36(12):7007-7010.
［10］谢利,余德海,申涛.辛开苦降甘补调和法治疗老年晚期非小细胞肺癌的临床研究［J］.成都中医药大学学报,2019,42(4):45-48.
［11］Baek KS,Yi YS,Son YJ,et al.In vitro and in vivo antiinflammatory activities of Korean Red Ginsengderived components［J］.Journal of Ginseng Research,2016,40(4):437-444.
［12］Baek KS,Yi YS,Son YJ,et al.Comparison of anticancer activities of Korean Red Ginsengderived fractions［J］.Journal of Ginseng Research,2017,41(3):386-391.
［13］Choi MR,Kwak SM,Bang SH,et al.Chronic saponin treatment attenuates damage to the pancreas in chronic alcoholtreated diabetic rats［J］. Journal of Ginseng Research,2017,41(4):503-512.
［14］LópezLázaro M.Distribution and biological activities of the flavonoid luteolin［J］.Mini Reviews in Medicinal Chemistry，2009,9(1):31-59.
［15］邓东沅,顾立刚,刘晓婷,等.木犀草素体外对H1N1感染A549诱导凋亡的干预作用及机制［J］.中华中医药杂志,2017,32(4):1524-1527.
［16］吴莹,金叶智,吴珺,等.汉黄芩素对流感病毒感染肺泡巨噬细胞炎症相关因子的影响［J］.中国病理生理杂志,2011,27(3):533-538.
［17］Chen Y,Nie YC,Luo YL,et al.Protective effects of naringin against paraquat-induced acute lung injury and pulmonary fibrosis in mice［J］.Food and chemical toxicology,2013,58:133-140.
［18］王高举,焦红军.基于网络药理学和分子对接技术的热毒宁注射液抗SARS、MERS和COVID-19的潜在共性作用机制与活性成分研究［J］.药物评价研究,2022,45(5):842-852.
［19］王春霞,胡晓东,许斌，等.靶向新型冠状病毒SARS-CoV-2主蛋白酶的抗病毒药物研究进展［J］.有机化学,2022,42(7):1974-1999.
［20］Hewitson TD,Mookerjee I,Masterson R,et al.Endogenous relaxin is a naturally occurring modulator of experimental renal tubulointerstitial fibrosis［J］.Endocrinology,2007,148(2):660-669.
［21］Chen L,Sha ML,Li D,et al.Relaxin abrogates renal interstitial fibrosis by regulating macrophage polarization via inhibition of Tolllike receptor-4 signaling［J］.Oncotarget,2017,8(13):21044-21053.
［22］Kindrachuk J,Ork B,Hart BJ,et al.Antiviral potential of ERK/MAPK and PI3K/AKT/mTOR signaling modulation for Middle East respiratory syndrome coronavirus infection as identified by temporal kinome analysis［J］.Antimicrob Agents Chemother,2015,59:1088-1099.
［23］Zhou BX,Yang ZF,Feng QT,et al.Aurantiamide acetate from Baphicacanthus cusia root exhibits antiinflammatory andantiviral effects via inhibition of the N-κB signaling pathway in Influenza a virus-infected cells ［J］.Journal of Ethnopharmacology,2017,199:60-67.
［24］Alsamman AM,Zayed H.The transcriptomic profiling of SARS-CoV-2 compared to SARS,MERS,EBOV,and H1N1［J］.PLoS One,2020,15(12):e0243270.
［25］Wu Y,Wang F,Shen C,et al.A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2［J］.Science,2020,368(6496):1274-1281.
［26］国家卫生健康委.关于印发新冠肺炎出院患者主要功能障碍康复治疗案的通知［EB/OL］.(2020-05-13)［2022-05-20］.http://www.gov.cn/zhengce/zhengceku/ 202005/15/content_5511843.htm.

相似文献/References:

[1]严谨 王芙蓉 史海龙.基于网络药理学探讨“葛根 －黄芩”药对治疗糖尿病作用机制分析[J].陕西中医药大学学报,2018,(05):173.[doi:DOI:10．13424/j．cnki．jsctcm．2018．05．051]
[2]李正欢 宋雪利 王飞 陈杨梁杨.浅论基于苗医理论的广谱抗肿瘤复方参棱夏[J].陕西中医药大学学报,2020,(5):066.[doi:10.13424/j.cnki.jsctcm.2020.05.017]
　Li Zhenghuan,Song Xueli,Wang Fei,et al.A Discussion on the Broad - spectrum Antitumor Compound Shen Leng Xia Based on Theories of Miao Medicine[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(05):066.[doi:10.13424/j.cnki.jsctcm.2020.05.017]
[3]陈奕晨 王娜 王文杰**.淫羊藿抗抑郁作用机制的网络药理学研究[J].陕西中医药大学学报,2021,44(02):053.[doi:10.13424/j.cnki.jsctcm.2021.02.012]
　CHEN Yi - chen,WANG Na,WANG Wen jie.Network Pharmacology Study on Antidepressant Mechanism of Epimedium[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(05):053.[doi:10.13424/j.cnki.jsctcm.2021.02.012]
[4]乐世俊 白雪 黄昱曦 王文晓 高欢 唐于平.羟基红花黄色素A治疗心脑血管疾病作用机制的网络药理学研究[J].陕西中医药大学学报,2021,44(05):106.[doi:10.13424/j.cnki.jsctcm.2021.05.022]
　LE Shijun?BAI Xue?HUANG Yuxi?WANG Wenxiao?GAO Huan?TANG Yuping.Mechanism Research of Hydroxysafflower Yellow A in The Treatment of Cardiovascular and Cerebrovascular Diseases by Network pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(05):106.[doi:10.13424/j.cnki.jsctcm.2021.05.022]
[5]胡越 许兵兵.网络药理学预测人参抗抑郁作用机制[J].陕西中医药大学学报,2021,44(05):113.[doi:10.13424/j.cnki.jsctcm.2021.05.023]
　HU Yue XU Bingbing.Prediction of Antidepressant Mechanism of Ginseng by Network Pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(05):113.[doi:10.13424/j.cnki.jsctcm.2021.05.023]
[6]汪宗清 侯卫 聂红科 宋娜 王兴强 汤小虎彭江云.基于网络药理学和分子对接探讨温阳通络方治疗类风湿关节炎的作用机制[J].陕西中医药大学学报,2021,44(05):120.[doi:10.13424/j.cnki.jsctcm.2021.05.024]
　WANG Zongqing HOU Wei NIE Hongke SONG NaWANG Xingqiang TANG XiaohuPENG Jiangyun.Discussion on Wenyang Tongluo Recipe Mechanism of Rheumatoid Arthritis Treatment Based on Network Pharmacology and Molecular Docking[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(05):120.[doi:10.13424/j.cnki.jsctcm.2021.05.024]
[7]于鲲鹏 于永铎.基于网络药理学讨论白花蛇舌草治疗溃疡性结肠炎的作用机制[J].陕西中医药大学学报,2022,(03):087.[doi:10.13424/j.cnki.jsctcm.2022.03.022]
　YU Kunpeng YU Yongduo.Discussion on Mechanism of Hedyotis diffusa in Treatment of Ulcerative Colitis Based on Network Pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2022,(05):087.[doi:10.13424/j.cnki.jsctcm.2022.03.022]
[8]程江雪 武旭 郭东艳 史亚军 邹俊波 吴睿彬 张小明.基于网络药理学研究当-归赤芍药对治疗心绞痛作用机制[J].陕西中医药大学学报,2022,(03):097.[doi:10.13424/j.cnki.jsctcm.2022.03.023]
[9]闫浩 刘潇潇 于春雪 孙轲强 成帆 王虎 宋逍.基于网络药理和分子对接探究黄芪葛根汤治疗糖尿病的作用机制[J].陕西中医药大学学报,2022,(04):126.[doi:10.13424/j.cnki.jsctcm.2022.04.028]
　YAN Hao LIU Xiaoxiao YU Chunxue SUN Keqiang CHENG Fan WANG Hu SONG Xiao.Study on Mechanism of Huangqi Gegen Decoction inTreating Diabetes Based on Network Pharmacology and Molecular Docking[J].Journal of Shaanxi University of Traditional Chinese Medicine,2022,(05):126.[doi:10.13424/j.cnki.jsctcm.2022.04.028]
[10]曹蓓苓 潘赐明 杨晓洁 范瀚中 李奇奇 夏雨欣 陈文慧.基于生物信息技术研究川芎-防风-麻黄角 药治疗缺血性脑卒中的机制[J].陕西中医药大学学报,2022,(06):091.[doi:10.13424/j.cnki.jsctcm.2022.06.018]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（82174330)；陕西省科技厅科研基金项目(2020ZDLSF05-15，2020SF-338，2022JQ-965)；陕西省科技厅创新团队项目（2022TD-55）；陕西省中管局基金项目（2021-02-ZZ-004）；陕西省中管局团队项目（2022-SLRH-LJ-002）；陕西省“特支计划”区域发展人才项目（2017）；陕西中医药大学学科创新团队建设项目（2019-YL05）；陕西中医药大学研究生质量提升工程专项项目（ZG004）
更新日期/Last Update: 2023-09-22