[1]陈奕晨 王娜 王文杰**.淫羊藿抗抑郁作用机制的网络药理学研究[J].陕西中医药大学学报,2021,44(02):053-60.[doi:10.13424/j.cnki.jsctcm.2021.02.012]
 CHEN Yi - chen,WANG Na,WANG Wen jie.Network Pharmacology Study on Antidepressant Mechanism of Epimedium[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(02):053-60.[doi:10.13424/j.cnki.jsctcm.2021.02.012]
点击复制

淫羊藿抗抑郁作用机制的网络药理学研究()
分享到:

《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
44
期数:
2021年02期
页码:
053-60
栏目:
出版日期:
2021-03-26

文章信息/Info

Title:
Network Pharmacology Study on Antidepressant Mechanism of Epimedium
文章编号:
2096-1340(2021)02-0053-08
作者:
陈奕晨 王娜 王文杰**
西安市精神卫生中心,陕西 西安 710100
Author(s):
CHEN Yi - chen WANG Na WANG Wen – jie
Department of pharmacy, Xi’an mental health center, Xi’an 710100, China
关键词:
淫羊藿抑郁症网络药理学作用靶点蛋白相互作用
Keywords:
Epimedium Depression Network pharmacology Target Protein interaction
分类号:
R282.71
DOI:
10.13424/j.cnki.jsctcm.2021.02.012
文献标志码:
A
摘要:
目的通过构建淫羊藿有效成分的靶点蛋白相互作用网络和活性成分作用靶点的网络,对靶点相关的通路和功能进行分析,研究淫羊藿抗抑郁的作用机制? 方法 采用 Traditional Chinese Medicine Systems Pharma-cology(TCMSP)数据库查询获取淫羊藿主要的活性成分,通过 drugbank 获取各成分的作用靶点,使用 Cytoscape软件构建活性成分-作用靶点的网络,利用 GeneCards?OMIM?NCBI 等数据库查询抑郁症相关的靶点,然后将淫羊藿的靶点与抑郁症靶点进行映射? 使用 String 数据库和 Cytoscape 软件绘制靶点蛋白相互作用网络,采用 DA-VID 数据库对靶点进行 GO 及 KEGG 通路分析? 结果 经过筛选得到淫羊藿的活性成分 21 个,主要涉及的作用靶点有 188 个? 网络分析结果表明,淫羊藿发挥抗抑郁药效过程中主要涉及毒性物质反应?金属离子反应?细胞外刺激反应?氧化应激反应等生物过程,通过调节 PI3K - Akt signaling pathway?TNF signaling pathway?IL -17 sig-naling pathway 等信号通路来发挥抗抑郁作用? 结论 本研究结果表明淫羊藿抗抑郁具有多成分-多靶点-多途径的特点,为淫羊藿抗抑郁作用机制的研究提供了新的方法?思路和科学的理论指导。
Abstract:
Objective To study the antidepressant mechanism of Herba Epimedii by constructing the target protein in-teraction network of active ingredients and the target network of active ingredients. MethodsThe traditional Chinese medi-cine systems pharmacy (tcmsp) database was used to search for the main active components of Epimedium, and the target of each component was obtained through drugbank. The network of active component targets was constructed by Cytoscape software. The depression related targets were searched by genecards, OMIM, NCBI and other databases, and then the tar-gets of Epimedium were mapped to depression targets. String database and Cytoscape software were used to draw the target protein interaction network, and David database was used to analyze the go and KEGG pathways. Results 21 active com- ponents of Epimedium were screened, and 188 targets were involved. The results of network analysis showed that the anti-depressant effect of Epimedium mainly involved in toxic substance reaction, metal ion reaction, extracellular stimulation reaction, oxidative stress reaction and other biological processes. The antidepressant effect was exerted by regulating PI3K Akt signaling pathway, TNF signaling pathway, IL -17 signaling pathway and other signaling pathways. Conclusion The results of this study show that the antidepressant effect of Epimedium has the characteristics of multi - component, multi -target and multi - channel, which provides a new method, idea and scientific theoretical guidance for the study of antide-pressant mechanism of Epimedium.

参考文献/References:

[1] Cavanagh JTO, Carson AJ, Sharpe M, et al. Psychologi-cal autopsy studies of suicide: a systematic review[J].Psychol Med, 2003,(33):395 – 405.
[2] Liu Han, He YanLing, Wang JingYi, et al. Epidemiology of depression at Traditional Chinese Medicine Hospital in Shanghai,China[J]. Comp Psychiatry, 2016,(65):1 –8.
[3] Berton O, Nestler EJ. New approaches to antidepressant drug discovery: beyond monoamine [J]. Nat Rev Neu-rosci 2006,(7):137 – 151.
[4] Trivedi MH, Rush AJ, Wisniewski SR, et al. Evaluation of outcomes with citalopram for depression using measure-ment - based care in STAR?D: implications for clinical practice[J]. Am J Psychiatry,2006,(163):28 – 40.
[5]钟海波,潘颖,孔令东. 淫羊藿提取物抗抑郁作用研究[J]. 中草药,2005,36(10):1506 -1510.
[6] Hu YL, Lv DH, Song DL. Effect of Epimedium propilis adjuvmant ( EPA) onactivity of celiac macrophages in chicks and mice[J]. China Journal of veterinary Science and Technology,1999, 5: 8 -11.
[7] Zhang Wenjing, Chen Haixia, Wang Zhaoshuai, et al. Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium ko-reanum Nakai [ J]. Food Sci Technol, 2013, 50 (6):1122 -1129.
[8] Luo GJ, Ci XX, Ren R , et al. Isolationols from Epime-dium brevicornum andtheir Effects of Two New Prenylfla-von on Cytokine Production in vitro [ J]. Planta Med,2009, 75: 843 -847.
[9] Li F, Gong QH, Wu Q, et al. Icariin isolated from Epni-medium brevicornum Maxim attenuates learning and mem-ory deficits induced by D - galactose in rats[J]. Pharma-cology Biochemistry and Behavior. 2010, 96: 301 -305.
[10]Hopkins A L. Network pharmacology: the next paradigm in drugdiscovery[J]. Nat Chem Biol,2008,4( 11) : 682 -90.
[11]罗武龙,陈洁,牛婕,等. 基于网络药理学的二仙汤治疗抑郁症的作用机制研究[J]. 中国药理学通报,2020,36(9) :1317 -1324.
[12]牟海栋,屠鹏飞,姜勇 . 基于网络药理学的肉桂温经通脉的作 用 机 制 研 究[J]. 药 学 学 报 ,2016,51(11):1724 -1733.
[13]范毓慧,赵迪,刘欢乐,等. 基于网络药理学技术的肉苁蓉治疗便秘的分子机制研究[J]. 山西大学学报(自然科学版),2020:1 -12.
[14]Wu H N,YU G H,Zhao F Z,et al. Mining and discovery for active components of " Coptidis Rhizome – Magnoliae Officinalis Cortex" based on network pharmacology [J] .Zhongguo Zhong Yao Za Zhi,2019,44 (4):803 -810.
[15] Xu Xue, Zhang Wuxia, Huang Chao, et al. A Novel Chemometric Method for the Prediction of Human Oral Bioavailability[J]. international journal of molecular sci-ences,2012,(13), 6964 -6982.
[16]Chen G,Huang C,Liu Y,et al. A Network Pharmacology Approach to Uncover the Potential Mechanism of Yinchensini Decoction[ J ] . Evid Based Complement Alternat Med,2018:2178610.
[17] Walters, WP;Murcko, MA. Prediction of ‘Drug - Like-ness’ [J] . advanced drug delivery reviews, 2002,54:255 – 271.
[18]Smoot Me,Ono K,Rusheinski J,et al. Cytoscape 2. 8: new features for data integration and network visualization[ J ] . Bioinformatics,2011,27 (3):431 -432.
[19]郝俊霞,高梓森,高皓,等. 基于网络药理学的雷公藤肾毒性机制探讨[ J ] . 中国实验方剂学杂志,2019,25( 16):142 -151.
[20]刘元月,胡丹,嵇云,等. 基于网络药理学探讨越鞠丸快速抗抑郁作用机制[J]. 辽宁中医药大学学报,2020,22(11):145 -149.
[21]Dennis G,Sherman BT,Hosack DA,et al. DAVID:Data-base for Annotation,Visualization,and Integrated Discov-ery[ J ] . Genome Biol,2003,4 ( 5 ):3.
[22] 李刚,许波,梁学振,基于网络药理学研究淫羊藿抗骨质疏松的分子机制[J]. 中国药理学通报,2018,34(2): 267 -273.
[23] 张笑笑. 淫羊藿对产前应激子鼠抑郁样行为及脑ⅠmGluRs?EAAT2 表 达 的 影 响 [ D]. 西 安: 西 北 大学. 2017.
[24] 吴丹, 高耀,向欢,等. PI3K/ Akt 信号通路在抑郁症及抗抑郁中药作用机制研究中的进[J]. 中草药,2019,50(18):4461 -4469.
[25]初倩,阎兆君,黄巧艺,等. 巴戟天 - 苍术药对治疗青少年抑郁症的网络药理学研究[J]. 湖南中医药大学学报,2020,40(11):1371 -1378.

相似文献/References:

[1]刘 娟,田  磊,王  曼,等.电针对慢性应激抑郁模型大鼠脑组织中大麻素受体CB1的影响[J].陕西中医药大学学报,2019,(03):064.[doi:10.13424/j.cnki.jsctcm.2019.03.018]
 Liu Juan Tian Lei Wang Man Lu Jian Lei Zhengquan.Effect of Electroacupuncture on Cannabinoid Receptor CB1 in Brain Tissue of Rats with Chronic Stress Depression[J].Journal of Shaanxi University of Traditional Chinese Medicine,2019,(02):064.[doi:10.13424/j.cnki.jsctcm.2019.03.018]
[2]侯 文,柏鲁宁,赵晓平,等.健脾解郁法在脑卒中后肝郁脾虚型抑郁症中的临床应用[J].陕西中医药大学学报,2019,(03):082.[doi:10.13424/j.cnki.jsctcm.2019.03.022]
[3]曾霖1 李惠林2 赵恒侠2 刘德亮2 楚淑芳2 指导:张学文3.张学文国医大师从肝论治抑郁症经验[J].陕西中医药大学学报,2019,(01):009.[doi:10.13424/j.cnki.jsctcm.2019.01.003]

备注/Memo

备注/Memo:
基金项目:陕西省科技计划自然科学基础研究项目(2017JQ8013)
通讯作者:王文杰,主管中药师 E - mail: wangwenjie8785@163. com
更新日期/Last Update: 2021-03-29