[1]闫浩 刘潇潇 于春雪 孙轲强 成帆 王虎 宋逍.基于网络药理和分子对接探究黄芪葛根汤治疗糖尿病的作用机制[J].陕西中医药大学学报,2022,(04):126-132.[doi:10.13424/j.cnki.jsctcm.2022.04.028]
　YAN Hao LIU Xiaoxiao YU Chunxue SUN Keqiang CHENG Fan WANG Hu SONG Xiao.Study on Mechanism of Huangqi Gegen Decoction inTreating Diabetes Based on Network Pharmacology and Molecular Docking[J].Journal of Shaanxi University of Traditional Chinese Medicine,2022,(04):126-132.[doi:10.13424/j.cnki.jsctcm.2022.04.028]
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基于网络药理和分子对接探究黄芪葛根汤治疗糖尿病的作用机制
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2022年04期
页码:
126-132
栏目:
论著
出版日期:
2022-07-20

文章信息/Info

Title:
Study on Mechanism of Huangqi Gegen Decoction inTreating Diabetes Based on Network Pharmacology and Molecular Docking
文章编号:
2096-1340(2022)04-0126-07
作者:
闫浩 刘潇潇 于春雪 孙轲强 成帆 王虎 宋逍
陕西中医药大学，陕西 咸阳 712046
Author(s):
YAN Hao LIU Xiaoxiao YU Chunxue SUN Keqiang CHENG Fan WANG Hu SONG Xiao
Shaanxi University of Chinese Medicine，Shaanxi Xianyang 712046,China
关键词:
关键词：黄芪葛根汤; 糖尿病; 网络药理学; 分子对接; 作用机制
Keywords:
Key words：Huangqi Gegendecoction; Diabetes; Network pharmacology; Molecular docking; Mechanism of action
分类号:
R285
DOI:
10.13424/j.cnki.jsctcm.2022.04.028
文献标志码:
A
摘要:
摘 要：目的 通过网络药理和分子对接探讨黄芪葛根汤治疗糖尿病的作用机制。方法 基于中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP）数据库筛选黄芪葛根汤有效成分及靶点基因，通过GeneCards和OMIM查找疾病的相关靶点基因，将药物有效成分靶标与疾病靶标映射；采用Cytoscape构建药物-成分-靶点-疾病网络图。运用STRING数据库，分析蛋白蛋白互作核心基因。将活性成分与核心基因的前3个做分子对接。利用Bioconductor进行GO功能富集和KEGG通路富集分析。结果 通过筛选得到黄芪葛根汤的活性成分59个，药物与疾病共同作用靶点135个；蛋白互作网络中核心基因为EGFR，IL6，MYC涉及Prostate cancer,p53 signaling pathway,Fluid shear stress and atherosclerosis,AGERAGE signaling pathway indiabetic complications等通路。结论 初步揭示了黄芪葛根汤是以多成分、多靶点、多通路的复杂形式发挥治疗糖尿病作用，为黄芪葛根汤的临床应用提供理论依据。
Abstract:
Abstract： Objective To explore the mechanism of Huangqi Gegen decoction in the treatment of diabetes through network pharmacology and molecular docking. Methods Based on the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) database，the effective components and target genes of Huangqi Gegen decoction were screened. The disease related target genes were found through GeneCards and OMIM，and the drug effective component targets were mapped with disease targets；Cytoscape was used to construct drug component target disease network. Using STRING database，the core genes of proteinprotein interaction were analyzed. Connect the active ingredient with the first three of the core genes. Bioconductor was used for GO function enrichment and KEGG pathway enrichment analysis. Results 59 active components of Huangqi Gegen decoction and 135 common targets of drugs and diseases were obtained；The core genes in the protein'protein interaction network are EGFR，IL6 and MYC，which involve prostate cancer，p53 signaling pathway，Fluid shear stress and atherosclerosis，AGERAGE signaling pathway indiabetic composites and other pathways. Conclusion It is preliminarily revealed that Huangqi Gegen decoction plays a role in the treatment of diabetes in a complex form of multicomponent，multitarget and multi'channel，which provides a theoretical basis for the clinical application of Huangqi Gegen decoction.

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备注/Memo

备注/Memo:
基金项目：国家重点研发计划项目（2019YFC1711200）；陕西省自然科学基础研究计划项目（2019JQ-874）
更新日期/Last Update: 2022-07-25