[1]于鲲鹏 于永铎.基于网络药理学讨论白花蛇舌草治疗溃疡性结肠炎的作用机制[J].陕西中医药大学学报,2022,(03):087-96.[doi:10.13424/j.cnki.jsctcm.2022.03.022]
 YU Kunpeng YU Yongduo.Discussion on Mechanism of Hedyotis diffusa in Treatment of Ulcerative Colitis Based on Network Pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2022,(03):087-96.[doi:10.13424/j.cnki.jsctcm.2022.03.022]
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基于网络药理学讨论白花蛇舌草治疗溃疡性结肠炎的作用机制
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2022年03期
页码:
087-96
栏目:
论著
出版日期:
2022-05-26

文章信息/Info

Title:
Discussion on Mechanism of Hedyotis diffusa in Treatment of Ulcerative Colitis Based on Network Pharmacology
文章编号:
2096-1340(2022)03-0087-10
作者:
于鲲鹏1 于永铎2
1.辽宁中医药大学,辽宁 沈阳 110847;
2.辽宁中医药大学附属医院,辽宁 沈阳 110847
Author(s):
YU Kunpeng1 YU Yongduo2
1.Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;
2.Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China
关键词:
关键词:溃疡性结肠炎白花蛇舌草网络药理学作用机制信号通路
Keywords:
Key words: Ulcerative colitisHedyotis diffusaNetwork pharmacologyMechanism of actionSignal pathway
分类号:
R285.5
DOI:
10.13424/j.cnki.jsctcm.2022.03.022
文献标志码:
A
摘要:
摘 要:目的 基于网络药理学技术分析讨论白花蛇舌草治疗溃疡性结肠炎(ulcerative colitis,UC)的潜在治疗靶标及作用机制。方法 通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛查白花蛇舌草有效成分以及对应靶点。利用GeneCards,CTD,DisGeNET数据库,以“ulcerative colitis”为检索词查询疾病靶点蛋白,得到疾病相关靶点。使用Venny2.1.得出白花蛇舌草与UC的共同靶点作为预测靶点,利用STRING数据库构建PPI蛋白互作网络,并利用Cytoscape构建白花蛇舌草治疗UC的药物-有效成分-靶点网络图。应用Cluster Profiler R包进行GO功能富集分析和KEGG信号通路分析,并进行可视化作图。结果 ①经筛选分析得出白花蛇舌草有效化合物7个,对应的靶基因123个,治疗UC的预测靶基因17017个。药物与UC的相交靶点119个。②PPI网络中的核心基因主要有白细胞介素6(IL-6),血管内皮生长因子A(VEGFA),肿瘤蛋白P53(TP53),肿瘤坏死因子(TNF)等。③GO功能富集分析显示白花蛇舌草对UC的预测靶基因所涉及的生物学过程(BP)主要有细胞对药物的反应,对营养水平的反应,活性氧代谢过程,对活性氧的反应等;细胞组分(CC)包括膜筏,膜区,微膜区,突触膜,突触后膜的组成部分等;分子功能(MF)主要涉及到神经递质受体活性,G蛋白偶联胺受体活性,丝氨酸水解酶活性,丝氨酸型内肽酶活性等。KEGG通路富集分析结果显示有糖尿病并发症中的AGE-RAGE信号通路,松弛素信号通路,IL-17信号通路,TNF信号通路,HIF-1信号通路等主要通路可能与白花蛇舌草治疗UC相关。结论 白花蛇舌草可能通过IL-6,VEGFA,TP53,TNF,EDF,JUN,MAPK1等多靶点,以及AGE RAGE信号通路、松弛素信号通路,HIF-1信号通路,IL-17信号通路,PI3K-Akt信号通路等通路共同发挥作用而治疗UC,为后续深入研究打下理论基础。
Abstract:
Abstract: Objective To analyze and discuss the potential therapeutic targets and mechanism of Hedyotis diffusa in the treatment of ulcerative colitis based on network pharmacological technology.Methods The effective components and corresponding targets of Hedyotis diffusa were screened by TCMSP.Using GeneCards,CTD,DisGeNET databases,the disease target proteins were queried with “ulcerative colitis” as the key word,and the disease-related targets were obtained.Use Venny 2.1,the common target of Hedyotis diffusa and ulcerative colitis was obtained as the prediction target,the PPI protein interaction network was constructed by using STRING database,and the drug active component target network diagram of Hedyotis diffusa in the treatment of ulcerative colitis was constructed by using Cytoscape.Cluster Profiler R package is used for GO function enrichment analysis and KEGG signal pathway analysis,and visual drawing is carried out.Results ①After screening and analysis,there were 7 effective compounds of Hedyotis diffusa,123 corresponding target genes and 17017 predicted target genes for the treatment of UC.There were 119 intersecting targets between drugs and ulcerative colitis.②The core genes in PPI network mainly include interleukin 6 (IL6),vascular endothelial growth factor A (VEGFA),tumor protein p53 (TP53),tumor necrosis factor (TNF),etc.③GO functional enrichment analysis showed that the biological process (BP) involved in the prediction target gene of Hedyotis diffusa to UC mainly included cell response to drugs,response to nutritional level,reactive oxygen species metabolism process,response to reactive oxygen species,etc;Cell components (CC) include membrane raft,membrane area,micromembrane area,synaptic membrane,components of postsynaptic membrane,etc;Molecular function (MF) mainly involves neurotransmitter receptor activity,G protein coupled amine receptor activity,serine hydrolase activity,serine endopeptidase activity and so on.KEGG pathway enrichment analysis showed that AGE-RAGE signal pathway,relaxin signal pathway,IL-17 signal pathway,TNF signal pathway,HIF-1 signal pathway and other major pathways in diabetes complications may be related to the treatment of ulcerative colitis with Hedyotis diffusa.Conclusion Hedyotis diffusa may play a role in the treatment of ulcerative colitis through multiple targets such as IL6,VEGFA,TP53,TNF,EDF,JUN,MAPK1 as well as AGE RAGE signal pathway,relaxin signal pathway,HIF-1 signal pathway,IL-17 signal pathway and PI3K-Akt signal pathway,which lays a theoretical foundation for further research.

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备注/Memo

备注/Memo:
基金项目:辽宁省重点研发计划项目(2020JH2/10300062)
更新日期/Last Update: 2022-05-30