[1]刘燕 邹远荣 黄建萍 王红艳 夏泽冰 王七龙井亚江 彭亮 高静 张岗 王丹阳 颜永刚.UPLC-TOF-MS、GC-MS结合网络药理学探讨炙甘草抗心律失常的作用机理[J].陕西中医药大学学报,2023,(04):084-93.[doi:2096-1340(2023)04-0084-10]
　LIU Yan ZOU Yuanrong HUANG Jianping WANG Hongyan XIA Zebing WANG Qilong JING Yajiang PENG Liang GAO Jing ZHANG Gang WANG Danyang YAN Yonggang.Exploration of the Mechanism of Anti Arrhythmia Effect of Roasted Licorice by UPLC-TOF-MS,GC-MS Combined with Network Pharmacology[J].Journal of Shaanxi University of Traditional Chinese Medicine,2023,(04):084-93.[doi:2096-1340(2023)04-0084-10]
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UPLC-TOF-MS、GC-MS结合网络药理学探讨炙甘草抗心律失常的作用机理
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2023年04期
页码:
084-93
栏目:
实验研究
出版日期:
2023-07-20

文章信息/Info

Title:
Exploration of the Mechanism of Anti Arrhythmia Effect of Roasted Licorice by UPLC-TOF-MS,GC-MS Combined with Network Pharmacology
文章编号:
10.13424/j.cnki.jsctcm.2023.04.016
作者:
刘燕1 邹远荣2 黄建萍2 王红艳2 夏泽冰2 王七龙2井亚江2 彭亮2 高静2 张岗2 王丹阳3 颜永刚2
1.榆林市药品检验所，陕西 榆林 719000；
2.陕西中医药大学/陕西省秦岭中草药 应用开发工程技术研究中心/“秦药”研发重点实验室，陕西 咸阳 712046；
3.山东新时代药业有限公司，山东 临沂 273400
Author(s):
LIU Yan1 ZOU Yuanrong2 HUANG Jianping2 WANG Hongyan2 XIA Zebing2 WANG Qilong2 JING Yajiang2 PENG Liang2 GAO Jing2 ZHANG Gang2 WANG Danyang3 YAN Yonggang2
1.Yulin Institute for Drug Control,Shaanxi Yulin 719000,China;
2.Shaanxi University of Chinese Medicine/Shaanxi Qinling Traditional Chinese Medicine Application Development Engineering Technology Research Center/“Qin Yao” R&D Key Laboratory,Shaanxi Xianyang 712046,China;
3.Shandong New Era Pharmaceutical Co.,Ltd.,Shandong Linyi 273400,China
关键词:
关键词：炙甘草; 网络药理学; UPLC-TOF-MS; GC-MS; 心律失常
Keywords:
Key words： Roasted licorice; Network pharmacology; UPLC-TOF-MS; GC-MS; Arrhythmia
分类号:
R285.5
DOI:
2096-1340(2023)04-0084-10
文献标志码:
A
摘要:
摘 要：目的 将炙甘草的化学成分与网络药理学相结合，探讨炙甘草抗心律失常的作用机理。方法 运用UPLC-TOF-MS、GC-MS技术分析炙甘草的化学成分。利用TCMSP数据库筛选活性成分，通过Swiss Target Prediction数据库和Gene Cards数据库搜集药物作用靶点和疾病靶点，借助Cytoscape 3.8.2软件筛选核心靶点，对核心靶点进行蛋白互作网络分析，GO和KEGG富集分析，使用Cytoscape 3.8.2构建药物关键成分核心靶点通路图，分子对接验证由Autodocking vina 1.2.0软件完成。结果 共分析出289种化学成分，其中异甘草素、酪氨酸等16个成分为抗心律失常的关键成分；蛋白互作网络共筛选出ALB、AKT1、VEGFA、EGFR等55个核心靶点，GO富集共获得476个条目，KEGG共得到130条通路。分子对接结果表明，异甘草素、黄豆黄素、蜜桔黄素与核心靶点对接活性较好。结论 炙甘草可能通过调节ALB、AKT1、VEGFA、EGFR等多个靶点，以及参与癌症通路、癌症中的蛋白聚糖、内分泌耐药、EGFR酪氨酸激酶抑制剂耐药等信号通路发挥抗心律失常的作用。
Abstract:
Abstract： Objective To combine the chemical components of roasted licorice with network pharmacology and explore the mechanism of its anti arrhythmia effect.Methods UPLC-TOF-MS and GC-MS techniques were used to analyze the chemical components of roasted licorice.TCMSP database was used to screen active ingredients,Swiss Target Prediction database and Gene Cards database were used to collect drug action targets and disease targets,Cytoscape 3.8.2 software was used to screen core targets,and protein interaction network analysis,GO and KEGG enrichment analysis were carried out on core targets.Cytoscape 3.8.2 was used to construct drug key component core target pathway diagram,and Macromolecular docking verification was completed by Autodocking vina 1.2.0 software.Results A total of 289 chemical components were analyzed,among which 16 components such as isoliquiritigenin and tyrosine were key components for anti arrhythmias;The protein interaction network screened 55 core targets,including ALB,AKT1,VEGFA,EGFR,etc.GO enrichment resulted in a total of 476 entries,and KEGG resulted in a total of 130 pathways.The results of Macromolecular docking showed that isoliquiritigenin,daidzein and citrinin had good docking activity with the core target.Conclusion Roasted licorice may play an antiarrhythmic role by regulating multiple targets such as ALB,AKT1,VEGFA,and EGFR,as well as participating in cancer pathways,Proteoglycan in cancer,endocrine resistance,and resistance to EGFR Tyrosine kinase inhibitor.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（81973592）； 陕西省中医药管理局项目（2021-GJ-JC004）
更新日期/Last Update: 2023-07-29