[1]黄廓均.莪术挥发性成分的 ＧＣ－ＭＳ分析[J].陕西中医药大学学报,2020,(4):068-73.[doi:10.13424/j.cnki.jsctcm.2020.04.015]
　Huang Kuojun.A GC - MS Analysis of the Volatile Components of Curcuma Zedoaria[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(4):068-73.[doi:10.13424/j.cnki.jsctcm.2020.04.015]
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莪术挥发性成分的 ＧＣ－ＭＳ分析()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2020年4期
页码:
068-73
栏目:
出版日期:
2020-07-15

文章信息/Info

Title:
A GC - MS Analysis of the Volatile Components of Curcuma Zedoaria
文章编号:
2096-1340(2020)04-0068-06
作者:
黄廓均1; 2
1.湖北省中医院,湖北武汉430061;
2.湖北省中医药研究院,湖北武汉430074
Author(s):
Huang Kuojun 1; 2
1. Hubei Hospital of Traditional Chinese Medicine, Wuhan Hubei, 430061;
2. Hubei Academy of Traditional Chinese Medicine, Wuhan Hubei, 430074
关键词:
莪术; 挥发油; 气相色谱 －质谱联用; 定性分析; 质量控制
Keywords:
rhizoma zedoariae;  volatile oil;  gas chromatography - mass spectrometry;  qualitative analysis;  the quality control
分类号:
R282.6
DOI:
10.13424/j.cnki.jsctcm.2020.04.015
文献标志码:
Ａ
摘要:
目的对莪术挥发性成分进行定性分析,探究莪术挥发油的主要组成?方法采用气相-质谱联用色谱法进行定性分析,使用Agilent19091S-433:93.92873HP-5MS 5%Phenyl Methyl silox -60℃ -325℃( 325℃)( 0. 25μm ×0. 25 mm ×30 m) 色谱柱;程序升温:初始温度68℃,保持3min,以5℃/min升至160℃,以7℃/min升至220℃,保持15min;载气流量2.0mL/min;分流进样,进样口温度:250℃,进样量1μL;GC-MS用载气为高纯氦气(≥99.999%),质谱条件为EI源,轰击电压70eV,正离子模式,质量扫描范围50~550amu,离子源温度230℃?结果分析5个批次的莪术挥发油样品后,鉴定出了44种成分?结论莪术挥发油成分复杂,其中主要组成成分为烯酮?烯烃?芳香烃类物质,其中表莪术呋喃烯酮?莪术二酮?吉马酮?莪术烯?β-榄香烯含量较为丰富,可为莪术挥发油及其相关制剂的质量控制研究提供科学基础?
Abstract:
Objective To make a qualitative analysis of the volatile components of curcuma zedoaria in order to explore the ingredients of the volatile oil of the curcuma zedoaria. Method Gas - mass spectrometry was used for qualitative analysis by the chromatographic column of Agilent 19091S -433: 93. 92873 HP -5MS 5% Phenyl Methyl silox -60℃ -325℃( 325℃) ( 0. 25μm ×0. 25 mm ×30 m. temperature programming: to keep the initial temperature of 68℃ for 3 min and then raise the temperature to 160℃ at the speed of 5℃ / min, finally to 220℃ and remain for 15 min and the carrier gas flow rate is 2. 0ml / min. Shunt injection, inlet temperature: 250 ℃, injection volume: 1 curfew; The carrier gas used for GC - MS is high purity helium ( ≥99. 999%) . The mass spectrometry condition is EI source, the bombardment voltage is 70eV, the positive ion mode, the mass scanning range is 50 ~ 550 amu, and the ion source temperature is 230℃. Result After analyzing 5 batches of volatile oil samples, 44 components were identified. Conclusion The volatile oil of zedoary is complex in composition, among which the main components are enone, olefin and aromatic hydrocarbons. The contents of zedoary furanone, zedoary diketone, jermadone, zedoary and anyl - elemene are relatively rich, which can provide a scientific basis for the quality control of the volatile oil of zedoary and its related preparations.

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相似文献/References:

[1]黄廓均.莪术挥发性成分的 ＧＣ－ＭＳ分析[J].陕西中医药大学学报,2020,(01):069.[doi:10.13424/j.cnki.jsctcm.2020.01.018]
　Huang Kuojun,GC - MS Analysis of Volatile Components in Curcuma Zedoary[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(4):069.[doi:10.13424/j.cnki.jsctcm.2020.01.018]

备注/Memo

备注/Memo:
基金项目:湖北省食品药品监督检验研究院《湖北省中药材质量标准》《湖北省中药饮片炮制规范》新版编写项目(鄂食药监办文[2015]28号)
更新日期/Last Update: 2020-07-15