[1]黄廓均.莪术挥发性成分的 GC-MS分析[J].陕西中医药大学学报,2020,(01):069-75.[doi:10.13424/j.cnki.jsctcm.2020.01.018]
 Huang Kuojun,GC - MS Analysis of Volatile Components in Curcuma Zedoary[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(01):069-75.[doi:10.13424/j.cnki.jsctcm.2020.01.018]
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莪术挥发性成分的 GC-MS分析()
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《陕西中医药大学学报》[ISSN: 2096-1340/CN: 61-1501/R]

卷:
期数:
2020年01期
页码:
069-75
栏目:
出版日期:
2020-01-30

文章信息/Info

Title:
GC - MS Analysis of Volatile Components in Curcuma Zedoary
文章编号:
2096-1340(2020)01-0069-07
作者:
黄廓均
1.湖北省中医院,湖北 武汉 430061;2.湖北省中医药研究院,湖北 武汉 430074
Author(s):
Huang Kuojun 1 2
1. Hubei TCM Hospital, Wuhan China, 430061; 2. Hubei TCM Institute, Wuhan China, 430074
关键词:
莪术挥发油气相色谱 -质谱联用定性分析质量控制
Keywords:
curcuma zedoaria volatile oil GC - MS qualitative analysis quality control
分类号:
R277
DOI:
10.13424/j.cnki.jsctcm.2020.01.018
文献标志码:
A
摘要:
目的 对莪术挥发性成分进行定性分析,探究莪术挥发油的主要组成。方法 采用气相 -质谱联用色谱法进行定性分析,使用 Agilent19091S-433:93.92873HP-5MS5%PhenylMethylsilox-60℃ -325℃(325℃)(0.25μm×0.25mm×30m)色谱柱;程序升温:初始温度 68℃,保持 3min,以 5℃/min升至 160℃,以 7℃/min升至 220℃,保持 15min;载气流量 2.0mL/min;分流进样,进样口温度:250℃,进样量 1μL;GC-MS用载气为高纯氦气(≥99.999%),质谱条件为 EI源,轰击电压 70eV,正离子模式,质量扫描范围 50~550amu,离子源温度230℃。结果 分析 5个批次的莪术挥发油样品后,鉴定出了 44种成分。结论 莪术挥发油成分复杂,其中主要组成成分为烯酮、烯烃、芳香烃类物质,其中表莪术呋喃烯酮、莪术二酮、吉马酮、莪术烯、β-榄香烯含量较为丰富,可为莪术挥发油及其相关制剂的质量控制研究提供科学基础。
Abstract:
Objective To analyze the volatile components of curcuma zedoaria and explore the main components of the volatile oil. Methods Qualitative analysis was performed by GC - MS with Agilent 19091S - 433: 93. 92873 HP - 5MS 5% Phenyl Methyl silox -60 ℃ -325 ℃ ( 325 ℃) ( 0. 25μm × 0. 25 mm × 30 m) column; program temperature increase: initial temperature was 68 ℃, holding for 3 min, then increased to 160 ℃ at 5 ℃ / min, 220 ℃ at 7 ℃ / min,holding for 15 min; the carrier gas flow was 2. 0 mL/ min; split injection: injection Inlet temperature was 250 ℃, injection volume was 1 μL; the carrier gas for GC - MS is high purity helium ( ≥ 99. 999%) , the mass spectrum is EI source, the bombardment voltage is 70ev, the positive ion mode, the mass scanning range is 50 -550 amu, and the ion source temperature is 230 ℃. Results after analyzing 5 batches of curcuma zedoaria volatile oil samples, 44 components were identified. Conclusion The composition of volatile oil is complicated. The main components are ketene, olefin, and aromatic hydrocarbons. Among them, the content of epicurzereonoe, , curdione, germacrone, curzerene, β - elemene are abundant, which can provide scientific basis for the quality control research of curcuma zedoaria volatile oil and its related preparations.

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备注/Memo

备注/Memo:
基金项目:湖北省食品药品监督检验研究院《湖北省中药材质量标准》《湖北省中药饮片炮制规范》新版编写项目 (鄂食药监办文[2015]28号)
更新日期/Last Update: 2020-01-15