[1]邵奇 王倩梅 马善波 张伟 郭超 马忠英.姜黄素对小鼠急性肺损伤的保护作用[J].陕西中医药大学学报,2020,(4):063-67.[doi:10.13424/j.cnki.jsctcm.2020.04.014]
 Shao Qi,Wang Qianmei,Ma Shanbo,et al.A Research on the Protective Effect of Curcumin on Acute Lung Injury in Mice[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(4):063-67.[doi:10.13424/j.cnki.jsctcm.2020.04.014]
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姜黄素对小鼠急性肺损伤的保护作用()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2020年4期
页码:
063-67
栏目:
出版日期:
2020-07-15

文章信息/Info

Title:
A Research on the Protective Effect of Curcumin on Acute Lung Injury in Mice
文章编号:
2096-1340(2020)04-0063-05
作者:
邵奇1 王倩梅2 马善波3 张伟3 郭超3 马忠英3
1.铜川矿务局中心医院,陕西铜川727000;
2.空军军医大学西京医院,陕西西安710032;
3.空军军医大学西京医院,陕西西安710032
Author(s):
Shao Qi 11 Wang Qianmei 2 Ma Shanbo 3 Zhang Wei 3 Guo Chao 3 Ma Zhongying 33
1 Pharmacy Department in Central Hospital of Tongchuan Mining Bureau, Tongchuan Shaanxi, 727000;
2 Emergency department in Xijing Hospital of Air force Medical University, Xi’ an Shaanxi, 710032;
3 Pharmacy Department in Xijing Hospital of Air force Medical University, Xi’ an Shaanxi, 710032
关键词:
姜黄素脓毒性休克急性肺损伤炎症反应脂质过氧化反应
Keywords:
curcumin septic shock acute lung injury the inflammatory response Lipid peroxidation
分类号:
R282.5
DOI:
10.13424/j.cnki.jsctcm.2020.04.014
文献标志码:
摘要:
目的探讨姜黄素对小鼠急性肺损伤(Acutelunginjury,ALI)的保护作用?方法40只健康成年BALB/c小鼠被随机分为:对照组?模型组?姜黄素组和乌司他丁组?对照组腹腔注射生理盐水,其他各组腹腔注射脂多糖(LPS)20mg/kg建立急性肺损伤小鼠模型;姜黄素组在ALI造模前30min腹腔注射姜黄素200mg/kg;乌司他丁组于ALI造模前1h腹腔内注射乌司他丁(1×105U/kg)?12h后处死小鼠,留取血样,ELISA法测定小鼠血清中肿瘤坏死因子(Tumor necrosis factor, TNF - α)?白介素-6( Interleukin 6, IL -6)?丙二醛(Malondialdehyde, MDA) ?超氧化物歧化酶(Superoxide dismutase, SOD)水平的变化;留取肺组织,观察肺组织病理学变化并计算各组小鼠肺湿重/干重比值(W/D)?结果与对照组相比,模型组小鼠血清TNF-α?IL-6?MDA水平显著升高,SOD水平显著下降;而与模型组相比,姜黄素组和乌司他丁组小鼠血清TNF-α?IL-6?MDA水平明显降低,SOD水平显著升高?与对照组相比,模型组小鼠肺W/D显著升高;与模型组相比,姜黄素组和乌司他丁组小鼠肺W/D明显降低?模型组小鼠肺泡结构破坏严重,肺泡间隔增厚,间质渗出较多,而姜黄素组和乌司他丁组小鼠肺泡结构相对较完整,炎性细胞渗出较少?姜黄素组和乌司他丁组两组相比无差异?结论姜黄素可减轻LPS诱导的急性肺损伤小鼠炎症反应,其机制可能与抑制脂质过氧化反应有关?
Abstract:
Objective To explore the protective effect of curcumin on Acute LungIinjury ( ALI) in mice. Method 40 healthy adult BALB/ c mice were randomly divided into control group, model group, curcumin group and ulinastatin group. The control group was intraperitoneally injected with normal saline, and the other groups were intraperitoneally injected with lipopolysaccharide ( LPS) 20 mg/ kg to establish the mouse model of acute lung injury. The curcumin group was intraperitoneally injected with 200 mg/ kg of curcumin 30 minutes before the modeling of ALI. Ulinastatin group was intraperitoneally injected with ulinastatin ( 1 × 105 U/ kg) 1 hour before the establishment of the ALI model. After 12 hours, mice were sacrificed and blood samples were collected. The serum levels of tumor necrosis factor ( tnf - leutrium) , interleukin -6 ( il -6) , malondialdehyde ( MDA) , and superoxide dismutase ( SOD) were determined by ELISA. Lung tissue was retained to observe the histopathological changes and calculate the lung wet weight/ dry weight ratio ( W/ D) in each group. Result Compared with the control group, serum TNF - beam, il -6 and MDA levels of mice in the model group were significantly increased, and SOD levels were significantly decreased. Compared with the model group, the serum TNF - radiation, il - 6 and MDA levels of mice in the curcumin group and ulinastatin group were significantly reduced, and the level of SOD was significantly increased. Compared with the control group, lung W/ D was significantly increased in the model group. In contrst with the model group, the lung W/ D of mice in curcumin group and ulinastatin group was significantly reduced. The alveolar structure of mice in the model group was seriously damaged, the alveolar space was thickened, and the interstitial exudation was more, while the alveolar structure of mice in the curcumin group and ulinastatin group was relatively complete, and the inflammatory cells exudate less. There was no difference between curcumin group and ulinastatin group. Conclusion Curcumin can reduce the inflammatory response induced by LPS in mice with acute lung injury, and the mechanism may be related to the inhibition of lipid peroxidation.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81601671)
通讯作者:马忠英,主管药师?E-mail:312740711@qq.com
更新日期/Last Update: 2020-07-15