[1]穆学芳 管来顺.黄芪总苷提取物对心肌缺血再灌注损伤大鼠心肌细胞凋亡及相关蛋白 Ｂｃｌ－２表达的影响[J].陕西中医药大学学报,2017,(06):103-106.[doi:10.13424/j.cnki.jsctcm.2017.06.035]
　Mu Xuefang Guan Laishun.Effects of Astragaloside Extract on Cardiomyocyte Apoptosis and Expression of Bcl－2 in Myocardial Ischemia Ｒeperfusion Injury Ｒats[J].Journal of Shaanxi University of Traditional Chinese Medicine,2017,(06):103-106.[doi:10.13424/j.cnki.jsctcm.2017.06.035]
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黄芪总苷提取物对心肌缺血再灌注损伤大鼠心肌细胞凋亡及相关蛋白 Ｂｃｌ－２表达的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2017年06期
页码:
103-106
栏目:
实验研究
出版日期:
2017-11-01

文章信息/Info

Title:
Effects of Astragaloside Extract on Cardiomyocyte Apoptosis and Expression of Bcl－2 in Myocardial Ischemia Ｒeperfusion Injury Ｒats
文章编号:
2096－1340(2017)06－0103－04
作者:
穆学芳1 管来顺2
1． 陕西省第四人民医院心内科，陕西西安710043; 2． 陕西省人民医院急诊外科，陕西西安710068
Author(s):
Mu Xuefang1 Guan Laishun2
1．Department of Cardiology in The Fouth People’s Hospital in Shaanxi，Xi’an 710043，China; 2． Emergency Surgery in People’s Hospital in Shaanxi，Xi’an 710068． China
关键词:
黄芪总苷提取物;  心肌缺血再灌注;  心肌细胞凋亡;  Bcl－2;  大鼠
Keywords:
astragaloside extract;  myocardial ischemia reperfusion;  myocardial apoptosis;  Bcl－2;  rat
分类号:
Ｒ542.2
DOI:
10.13424/j.cnki.jsctcm.2017.06.035
文献标志码:
A
摘要:
目的探讨黄芪总苷提取物对大鼠实验性心肌缺血再灌注时心肌细胞凋亡以及对相关Bcl－2 蛋白表达的影响。方法将大鼠随机分为假手术组、缺血再灌注模型组、黄芪总苷提取物( 40mg /kg) 组，对左冠状动脉前降支进行结扎，造成类似于临床的心肌缺血再灌注损伤。分别对三组大鼠进行心肌细胞凋亡数目检测，Bcl－2 蛋白表达检测以及心肌损伤情况检测。结果与模型组大鼠相比较，黄芪总苷提取物组心肌细胞凋亡率明显降低( P＜0．05) ，Bcl－2 蛋白表达量明显降低( P＜0．05) ; 心肌组织损伤的病理学变化明显减轻。
Abstract:
Objective To observe the effects of astragaloside extract on cardiomyocyte apoptosis and expression of Bcl －2 in myocardial ischemia reperfusion injury rats． Method Ｒats were randomly divided into sham operation group，ischemical reperfusion group，astragaloside extract group ( 40mg /kg) ． The left anterior descending coronary artery was ligated to induce myocardial ischemia reperfusion injury similar to that in clinic． The number of myocardium apoptosis was detected in three groups，the expression of Bcl－2 protein and the myocardial injury were detected． Ｒesult Compared with the model group，the cardiac myocyte apoptosis rate in astragaloside extract group decreased significantly ( P＜ 0．05) ，the expression of Bcl－2 protein decreased significantly ( P＜ 0．05) ; pathological changes of myocardial tissue injury significantly reduced． Conclusion Astragaloside extract has protective effect on ischemia － reperfusion myocardium，and its mechanism may be related to down－regulation of Bcl－2 protein expression and further inhibition of cardiomyocyte apoptosis．

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017－04－24编辑:文颖娟
更新日期/Last Update: 2017-11-15