[1]王正引** 郭明章 陈燕.四物汤对血虚证小鼠肝细胞凋亡相关分子 Caspase3及 Caspase8表达的影响[J].陕西中医药大学学报,2017,(06):098-102.[doi:10.13424/j.cnki.jsctcm.2017.06.034]
 Wang Zhengyin Guo Mingzhang Chen Yan.Effects of Siwu Decoction on Expression of Caspase3 and Caspase8 in Hepatocytes of Mice with Blood Deficiency Syndrome[J].Journal of Shaanxi University of Traditional Chinese Medicine,2017,(06):098-102.[doi:10.13424/j.cnki.jsctcm.2017.06.034]
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四物汤对血虚证小鼠肝细胞凋亡相关分子 Caspase3及 Caspase8表达的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2017年06期
页码:
098-102
栏目:
实验研究
出版日期:
2017-11-01

文章信息/Info

Title:
Effects of Siwu Decoction on Expression of Caspase3 and Caspase8 in Hepatocytes of Mice with Blood Deficiency Syndrome
文章编号:
2096-1340(2017)06-0098-05
作者:
王正引1** 郭明章2 陈燕1
1.福建中医药大学药学院,福建福州350122; 2.福建中医药大学中医学院,福建福州350122
Author(s):
Wang Zhengyin1 Guo Mingzhang2 Chen Yan1
1.College of Pharmacy in Fujian University of Chinese Medicine,Fuzhou 350122,China; 2.TCM college in Fujian University of Chinese Medicine,Fuzhou 350122,China
关键词:
四物汤 血虚证 肝细胞 凋亡 半胱天冬氨酸蛋白酶
Keywords:
Decoction blood deficiency syndrome hepatocyte apoptosis cysteinyl aspartate specific protease
分类号:
R2-03;R289.5
DOI:
10.13424/j.cnki.jsctcm.2017.06.034
文献标志码:
A
摘要:
目的观察四物汤对血虚证小鼠肝细胞凋亡相关分子Caspase3 及Caspase8 表达的影响。方法24只C57BL/6J 小鼠随机分为空白组、模型组、给药组。以腹腔注射环磷酰胺建立血虚证模型,采用全自动血液分析仪检测外周血象,TUNEL 法检测肝细胞凋亡百分率,荧光定量PCR 法检测Caspase3 及Caspase8 mRNA 表达。Western blot 检测Caspase3 及Caspase8 蛋白表达。结果与模型组相比,给药组小鼠外周血红细胞数及血红蛋白含量升高( P<0.01) ,肝细胞凋亡百
Abstract:
Objective To obverse the effects of Siwu Decoction on expression of Caspase3 and Caspase8 in hepatocytes of mice with blood deficiency syndrome. Method 24 C57BL/6J mice were randomly divided into blank group, model group and administration group. The model of blood deficiency was established by intraperitoneal injection of cyclophosphamide. The peripheral hemogram was detected by automatic blood analyzer. The percentage of hepatocyte apoptosis was detected by TUNEL method. The expression of Caspase3 and Caspase8 mRNA was detected by fluorescence quantitative PCR. Western blot was used to detect the expression of Caspase3 and Caspase8 protein. Result Compared with the model group,the number of erythrocyte and the content of hemoglobin in the peripheral blood of the mice in the administration group increased ( P < 0. 01) ,the percentage of hepatocyte apoptosis decreased ( P < 0. 01) ,the expression of Caspase3 and Caspase8 mRNA and protein decreased ( P<0.05,P<0.01) . Conclusion Siwu Decoction can improve the peripheral hemogram of mice with blood deficiency syndrome,reduce the expression of Caspase3 and Caspase8,and lessen the apoptosis of hepatocytes.It is suggested that the mechanism of action of Siwu Decoction on decreasing hepatocyte apoptosis in blood deficiency syndrome model may be related to the inhibition of Caspase3 and activation of Caspase8 .

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-11-15编辑:文颖娟 ***
基金项目:国家自然科学基金青年科学基金项目(81202629);福建省卫生厅资助项目(wzy0615) 作者简介:王正引(1978-),男,讲师,医学博士,主要从事方剂配伍及临床应用研究。E-mail:johnzwang@163.com
更新日期/Last Update: 2017-11-15