[1]唐岚芳刘婷钟佳男杨莎莎许茜林友宁肖绍坚蔡晶△.苁蓉精对帕金森病模型大鼠延髓PI3K/AKT通路的影响[J].陕西中医药大学学报,2020,(02):070-74.[doi:10.13424/j.cnki.jsctcm.2020.02.018]
　Tang Lanfang,Liu Ting,Zhong Jianan,et al.Effects of Cistanche on PI3K / AKT Pathway in the Medulla Oblongata of Rat Model of Parkinson’ s Disease[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(02):070-74.[doi:10.13424/j.cnki.jsctcm.2020.02.018]
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苁蓉精对帕金森病模型大鼠延髓PI3K/AKT通路的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2020年02期
页码:
070-74
栏目:
出版日期:
2020-04-24

文章信息/Info

Title:
Effects of Cistanche on PI3K / AKT Pathway in the Medulla Oblongata of Rat Model of Parkinson’ s Disease
文章编号:
2096-1340(2020)02-0070-05
作者:
唐岚芳1刘婷1钟佳男1杨莎莎1许茜3林友宁2肖绍坚2蔡晶3△
1.福建中医药大学中西医结合研究院,福建福州350122;
2.福建中医药大学附属第三人民医院,福建福州350122;
3.福建中医药大学中西医结合学院,福建福州350122
Author(s):
Tang Lanfang 1;  Liu Ting 1 ;  Zhong Jianan 1 ;  Yang Shasha 1 ;  Xu Qian 3 ;  Lin Youning 2 ; Xiao Shaojian 2 ;  Cai Jing 3
1. Institute of Integrated Traditional Chinese and Western Medicine of Fujian University of Chinese Medicine,Fuzhou China, 350122;
2. The Third Affiliated People’ s Hospital of Fujian University of Chinese Medicine,Fuzhou China, 350122; 3. School of Integrated Traditional Chinese and Western Medicine of Fujian University of Chinese Medicine, Fuzhou China, 350122
关键词:
苁蓉精; 帕金森病; 延髓; PI3K/AKT通路; 神经元保护
Keywords:
cistanche;  Parkinson’ s Disease;  medulla oblongata;  PI3K / AKT pathway;  neuron protection
分类号:
R741
DOI:
10.13424/j.cnki.jsctcm.2020.02.018
文献标志码:
A
摘要:
目的研究苁蓉精对帕金森病模型大鼠延髓中PI3K/AKT通路的影响,探讨苁蓉精改善帕金森病相关症状可能的机制?方法将造模成功的24只SD大鼠随机分为模型组?苁蓉精40,80,160mg?Kg-1?d-1三个剂量组,并设立正常组与假手术组,每组6只;分别给予苁蓉精或生理盐水,连续灌胃14天?Western Blotting检测大鼠延髓AKT?PI3K及P-AKT?P-PI3K蛋白的表达?结果与正常组比较,PD模型组大鼠延髓中P-AKT?P-PI3K蛋白的表达量降低(P<0.05);与模型组对比,给药组PD模型大鼠中80?160mg?Kg-1?d-1剂量组的P-AKT蛋白的表达量明显增高(P<0.05),而40?80?160mg?Kg-1?d-1三个剂量组中P-PI3K蛋白的表达量都明显增高,且具有量效依赖关系(P<0.05)?结论:苁蓉精可提高延髓中P-AKT?P-PI3K蛋白的表达量对神经元起到保护作用?
Abstract:
Objective To study the effects of Cistanche on PI3K / AKT pathway in the medulla of rat model of Parkinson’ s Disease( PD) , and to explore the possible mechanism that cistanche improves the symptoms associated with Parkinson’s Disease. Methods Twenty - four SD rats successfully modeled were randomly divided into: model group, threegroups of different doses of hibiscus 40, 80 and 160 mg ? Kg-1 ? d -1 . and the normal group and sham operation group were set up, with 6 rats in each group. Cistanche or saline was administered to rats for 14 days. Western Blotting was used to detect the expression of AKT, PI3K and P - AKT, P - PI3K proteins in the rats’ medulla oblongata. Results Compared with the normal group, the expression of P - AKT and P - PI3K proteins in the medulla oblongata of the PD model group decreased ( P <0. 05) ; compared with the model group, the expression of P - AKT protein in the 80 and 160 mg? Kg-1 ? d -1 dose groups of the PD model rats was significantly increased ( P <0. 05) ; the expression of p - PI3K protein in three groups of 40, 80 and 160 mg ? Kg-1 ? d -1mg ? Kg-1 ?d-1was significantly increased, and there was a dose - dependent relationship ( P < 0. 05) . Conclusion Cistanche can increase the expression of P - AKT and P - PI3K proteins in the medulla oblongata and protect neurons.

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备注/Memo

备注/Memo:
基金项目:福建省科技厅引导项目(2017Y0053);福建省中医药科研项目(2017FJZYLC501,2017FJZYLC201,2017FJZYLC202);福建省自然基金项目(2018J01859,2018J01860)? 通讯作者:林友宁,主任医师?E-mail:105393388@qq.com 通讯作者:肖绍坚,副主任医师?E-mail:1678725605@qq.com 通讯作者:蔡晶,教授?E-mail:caij1@163.com
更新日期/Last Update: 1900-01-01