[1]彭 艳 白 雪 杨思进 冯文战 罗 刚 唐红梅.加味益气聪明汤对 VD 大鼠行为学变化及海马神经元突触结构的影响[J].陕西中医药大学学报,2017,(04):105-109113.[doi:10.13424/j.cnki.jsctcm.2017.04.034]
 Peng Yan Bai Xue Yang Sijing Feng Wenzhan Luo Gang Tang Hongmei.Influence of the Modified Yiqi Congming Decoction over Behavioral Changes and Synaptic Structure of the Hippocampus of VD Rats[J].Journal of Shaanxi University of Traditional Chinese Medicine,2017,(04):105-109113.[doi:10.13424/j.cnki.jsctcm.2017.04.034]
点击复制

加味益气聪明汤对 VD 大鼠行为学变化及海马神经元突触结构的影响()
分享到:

《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2017年04期
页码:
105-109113
栏目:
实验研究
出版日期:
2017-07-05

文章信息/Info

Title:
Influence of the Modified Yiqi Congming Decoction over Behavioral Changes and Synaptic Structure of the Hippocampus of VD Rats
文章编号:
2096-1340(2017)04-0105-05
作者:
彭 艳 白 雪 杨思进 冯文战 罗 刚 唐红梅
西南医科大学附属中医院,四川 泸州 646000
Author(s):
Peng Yan Bai Xue Yang Sijing Feng Wenzhan Luo Gang Tang Hongmei
The Affiliated Hospital of Southwest Medical University Hospital,Luzhou 646000,China
关键词:
加味益气聪明汤 血管性痴呆 突触
Keywords:
the modified Yiqi Congming DecoctionVDsynapse
分类号:
R743
DOI:
10.13424/j.cnki.jsctcm.2017.04.034
文献标志码:
A
摘要:
目的 观察加味益气聪明汤对血管性痴呆( VD) 大鼠行为学变化及海马神经元突触结构的影响。 方法 将 60 只实验大鼠随机分为假手术组及手术组,手术组包括模型组、尼莫地平组( 简称“对照 1 组”) 、复方 苁蓉益智胶囊组( 简称“对照 2 组”) 、加味益气聪明汤组( 简称“治疗组”) 。假手术组只分离出双侧颈总动脉, 使之充分暴露,不结扎,手术组采用永久性结扎双侧颈总动脉( 2 - VO) 法制备 VD 模型,对照 1 组给予尼莫地平 以 6. 25 mg·kg - 1 ·d - 1 给药,对照 2
Abstract:
Objective: To observe the effect of the modified Yiqi Congming Decoction on behavior changes and the synaptic structure of hippocampus neurons of VD ( vascular dementia) rats. Method: The 60 laboratory rats were randomly divided into sham - operated and operation group and the latter one includes the model group,the nimodipine ( the 1st control group) ,the compound Congrong Yizhi Capsule group ( the 2nd control group) and the modified Yiqi Congming group ( the treatment group) . The models in sham - operated group were just to completely expose carotid artery of both sides but not ligated,while VD models were to permanently conduct a ligation. The 1st control group received Nimodipine ( 6. 25 mg·kg -1 ·d -1 ) ,the 2nd one the compound Congrong Yizhi Capsule ( 0. 375 g·kg -1 ·d -1 ) , the treatment one the modified Yiqi Congming group ( 14. 58 g·kg - 1 ·d - 1 ) and the model and sham - operated ones were given lavage with same volumed distilled water for 30 days,2 mL/d. Morris water maze test could be used to check rats’behavior change and the electron microscope to observe synaptic ultrastructure changes of hippocampus neurons in all groups. Results: Water maze experimental results: Compared with the sham - operated group,rats’EL period obviously extended in model group ( P < 0. 01) and passing numbers and residence time of effective district as well as the platform were markedly reduced and shortened ( P < 0. 01) . Compared with sham - operated group,EL period of the 1st and 2nd group as well as the treatment group remarkablely shortened ( P < 0. 05 or P < 0. 01) and passing numbers and residence time of effective district as well as the platform were significantly increased and prolonged ( P < 0. 05 or P < 0. 01) . Electron microscopy results: In sham - operated group,rats’hippocampal synapse was highly dense and its structure was clear,its components and deep staining as well as high density in post - synapse dense area clearly identified. In the model group,mitochondrial cristae was becoming blurred and swelling within the presynaptic membrane,its structure lost of clarity,numbers reduced and light staining as well as low density in post - synapse dense area obvoiously seen. Compared with sham - operated group,synaptic numbers in rats’hippocampus district visibly reduced,its gap widened and its color in post - synapse dense area lightened in the model group; Compared with model group,in the 1st and 2nd group as well as the treatment group,synaptic numbers in rats’hippocampus district visibly increased,its gap narrowed,its color in post - synapse dense area darkened and the activity district lengthened. In comparison of two groups among the sham - operated,the 1st and 2nd as well as the treatment group,the above parameters changed a little. Conclusion: The modified Yiqi Congming Decoction can improve synaptic ultrastructure of hippocampus neurons in VD rats,thus improving the ability of learning and memory,which is similar to Nimodipine and the compound Congrong Yizhi Capsules.

参考文献/References:

[1]贾建平. 神经病学[M]. 北京: 人民卫生出版社,2013: 208 - 212.
[2]翟亮,李飞,杨骏. 针灸治疗血管性痴呆的临床研究进 展[J]. 陕西中医学院学报,2013,36( 3) : 122 - 125.
[3]赵勇,崔淑芳,汤球. 血管性痴呆动物模型研究进展 [J]. 上海实验动物科学,2005,25( 1) : 54 - 58.
[4]Morris R G,Garrud P,Rawlins J N,et al. . Place navigation impaired in rats with hippocampal lesions [J]. Nature,1982,297( 58) : 681 - 683.
[5]王维刚,周嘉斌,朱明莉,等. 小鼠动物实验方法系列 专题㈠—Morris 水迷宫实验在小鼠表型分析中的应用 [J]. 中国细胞生物学学报,2011,33( 1) : 8 - 14.
[6]李雪燕,陈开兵,张丽云. 当归多糖对衰老模型小鼠行 为学、血清和脑 SOD、端粒酶活性的影响[J]. 现代中 医药,2013,33( 2) : 101 - 104.
[7]赵建新,田元祥,李国明,等. 拟血管性痴呆小鼠模型皮 层及海马细胞病理组织学动态观察[J]. 中国病理生 理杂志,2000,16( 11) : 1214 - 1216.
[8] Sutter J,Scotto J C,Dufour H,et al. Can unwanted effects of activity regulating and stimulating therapy be eliminated.[J]. Therapeutique,1973,49( 2) : 103 - 104.
[9]王哲,张显,刘畅. 益智口服液对老年性痴呆大鼠模型 行为学、脑内胆碱乙酰转移酶及突触素水平的影响 [J]. 中国老年学杂志,2003,23( 3) : 189 - 191.
[10]黄敬,刘学源,梅元武,等. 缺血性痴呆大鼠行为学及 突触素改变的研究[J]. 中国临床神经科学,2007,15 ( 1) : 28 - 32.
[11]Hill I E,MacManus J P,Rasquinha I,et al. DNA fragmentation indicative of apoptosis following unilateral cerebral hypoxia - ischemia in the neonatal rat. [J]. Brain research,1995,676( 2) : 398 - 403.
[12]常富业,王永炎,高颖,等. 浅论开通玄府[A]. 中华 中医药学会内科分会. 2005 全国中医脑病学术研讨会 论文汇编[C]. 中华中医药学会内科分会,2005: 3.
[13]桂明进,鲍远程,张波. 益气聪明汤在神经内科疾病治 疗中的运用[J]. 中医药临床杂志,2012,24 ( 3) : 260 - 262.

相似文献/References:

[1]王 渊刘智斌 牛文民杨晓航 王 强刘思洋 李 杰鲁 刚 王卫刚.雷射针刺对血管性痴呆患者认知功能和血清基质金属蛋白酶 -9的影响[J].陕西中医药大学学报,2018,(01):052.[doi:10.13424/j.cnki.jsctcm.2018.01.017]
 Wang Yuan,Liu Zhibin,Niu Wenmin,et al.Effect of Laser Acupuncture on Cognitive Function and Serum Matrix Metalloproteinase - 9 in Patients with Vascular Dementia[J].Journal of Shaanxi University of Traditional Chinese Medicine,2018,(04):052.[doi:10.13424/j.cnki.jsctcm.2018.01.017]

备注/Memo

备注/Memo:
收稿日期:2016-08-11编辑:文颖娟白雪(1972-),女,硕士研究生导师,主任中医师,研究方向:中医药防治脑病的基础与临床研究。E- mail:bx7246@163.com。
更新日期/Last Update: 2017-07-15