[1] Liu Juan Tian Lei Wang Man Lu Jian Lei Zhengquan,.Effect of Electroacupuncture on Cannabinoid Receptor CB1 in Brain Tissue of Rats with Chronic Stress Depression [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (03): 64-67,85. [doi: 10.13424/j.cnki.jsctcm.2019.03.018]
Effect of Electroacupuncture on Cannabinoid Receptor CB1 in Brain Tissue of Rats with Chronic Stress Depression
- Article number:
- 2096-1340(2019)03-0064-04
- ZTFLH:
- R245. 3
- Code:
- A
- Abstract:
- Objective To observe the effect of electroacupuncture on cannabinoid system CB1 ( cannabinoid receptor 1) in rats with chronic stress depression, and to explore the mechanism of electroacupuncture on anti - depression. Methods Thirty healthy male SD rats were randomly divided into blank group, model group and electro – acupuncture group with 10 rats in each group. The model was established by the method of loneliness combined with chronic mild unpredictable stress ( CUMS) . The sucrose water intake and the space movement of open field test were observed. The expression of cannabinoid receptor CB1 in rat brain tissue was detected by immunohistochemistry. Results After treatment, the sucrose water intake and space movement of open field test in three groups were significantly lower in model group than in blank group ( P < 0. 01) , and higher in electro - acupuncture group than in model group ( P < 0. 05) ; the expression of CB1 in brain tissue of rats in model group was significantly lower than that in blank group ( P < 0. 01) ; the expression of CB1 in brain tissue of rats in the electro - acupuncture group was significantly higher than that in model group ( P < 0. 05) . Conclusion Electroacupuncture has obvious antidepressant effect, which may be achieved by regulating the endogenous cannabinoid receptor CB1 in rat brain.
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