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[1] He Lin Liu Han Gao Yuanxue Li Hao Hou Jianping Wang Bin,.Study on the Expression Pattern of 5 - LOX - CysLTs – CysLTsR and the Anti - inflammatory Mechanism of Baicalin – Germanuin on Cerebral Ischemia in Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (03): 57-63. [doi: 10.13424/j.cnki.jsctcm.2019.03.017]

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Study on the Expression Pattern of 5 - LOX - CysLTs – CysLTsR and the Anti - inflammatory Mechanism of Baicalin – Germanuin on Cerebral Ischemia in Rats

Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R] Volumes & Issues: 2019 03 pagination: 57-63 Column: Publication date: 2019-05-30

Article number:: 2096-1340(2019)03-0057-07

Author(s):: He Lin Liu Han Gao Yuanxue Li Hao Hou Jianping Wang Bin; Shaanxi University of Chinese Medicine, Xianyang China, 712046

Keywords:: cerebral ischemia; Baicali; Geniposide; 5 - LOX/ CysLTs/ CysLT pathway

ZTFLH:: R962A

DOI:: 10.13424/j.cnki.jsctcm.2019.03.017

Code:: A

Abstract:: Objective To dynamicly observe cysteinyl leukotriene ( CysLTs) activity in brain tissue of stroke rats and its 5 - lipoxygenase ( 5 - LOX) , cysteinyl leukotriene receptor 1 ( CysLTs1) , cysteinyl The change of expression level of leukotriene receptor 2 ( CysLTs2) , and to investigate the mechanism of anti - cerebral ischemic injury induced by baicalin - germangoside ( 7: 3) . Methods The model of middle cerebral artery occlusion simulated cerebral ischemia was prepared by suture embolism. The model was then randomly divided into blank control group ( control) , model group ( pMCAO) , baicalin - geniposide ( 7: 3) 30 mg/ kg, 45 mg/ kg, 60 mg/ kg groups, ziliutong 45 mg/ kg group and montelukast 0. 5 mg/ kg group. The treatment lasted 35 days. Neuroprotective effect was observed by neurological function score every7 days. The content of CysLTs in brain tissue was measured by ELISA, and the expression levels of 5 - LOX, CysLTs1 and CysLTs2 in brain tissue were detected by Western Blot. Results Compared with the model group, baicalin - geniposide ( 7: 3) 30 mg/ kg, 45 mg/ kg and 60 mg/ kg groups could significantly reduce the level of CysLTs within 28 days after cerebral ischemia; Western Blot results showed that the expression of 5 - LOX, CysLTs1 and CysLTs2 protein was significantly decreased; 35 days after recovery, there was no significant difference in the expression levels of each protein. Conclusion Baicalin - geniposide ( 7: 3) combination can alleviate neurological dysfunction in MCAO rats, and its mechanism may be related to inhibiting 5 - LOX activity, reducing the expression of CysLTs1 and CysLTs2 protein.

References:

[1] Bakthavachalam P, Shanmugam P S. Mitochondrial dysfunction - Silent killer in cerebral ischemia[ J] . J Neurol Sci,2017,375:417 -423.
[2]张锦,张允岭,郭蓉娟,等.从“毒损脑络”到“毒损络脉”的理论探讨[J].北京中医药,2013,32(7):483-486.
[3]程发峰.精制清开灵注射液对脑缺血的治疗作用及对兴奋性毒性级联损伤的调控机制[D].北京:北京中医药大学,2011.
[4]曾明飞,潘林梅,朱华旭,等.黄连解毒汤中栀子苷在正常及脑缺血大鼠体内药动学研究[J].中草药,2010,41(4):617-620.
[5]李敏,王斌,唐志书,等.黄芩苷?栀子苷对大鼠脑缺血保护作用的机制[J].中药药理与临床,2012,28(3):34-36.
[6]刘阿萍,李敏,王斌,等.黄芩苷和栀子苷配伍对大鼠脑缺血再灌注损伤的作用研究[J].中药药理与临床,2016,32(1):46-48.
[7]李敏,王斌,唐志书,等.栀子苷和黄芩苷配伍对脑缺血损伤急性期和恢复早期大鼠海马中CysLT2R蛋白及mRNA表达的影响[J].中药药理与临床,2012,28(5):18-20.
[8]刘寒,张欢欢,康亚国,等.黄芩苷?栀子苷配伍对脑缺血大鼠恢复期炎症因子的影响[J].陕西中医药大学学报,2018,41(3):75-81.
[9] Bederson J B, Pitts L H, Tsuji M, et al. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination[ J] . Stroke, 1986, 17( 3) :472 -476.
[10]朱晓磊.脑缺血损伤级联反应原理与胆酸?栀子甙配伍作用机制的研究[D].北京:北京中医药大学,2003.
[11] Zhou Y, Wei E Q, Fang S H, et al. Spatio - temporalproperties of 5 - lipoxygenase expression and activation inthe brain after focal cerebral ischemia in rats[ J] . LifeSci,2006,79( 17) : 1645 -1656.
[12] Borges T K, Alves E A, Vasconcelos H A, et al. Differences in the modulation of reactive species, lipid bodies,cyclooxygenase - 2, 5 - lipoxygenase and PPAR - gammain cerebral malaria - susceptible and resistant mice[ J] .Immunobiology,2017,222( 4) :604 -619.
[13]石巧娟,郭红刚,楼琦,等.半胱氨酰白三烯受体拮抗剂通过下调自噬减轻长爪沙鼠的全脑缺血再灌注损伤[J].中国实验动物学报,2018,26(1):57-64.
[14]张丽慧,赵建波,王艳芳,等.半胱氨酰白三烯受体与脑损伤关系的研究进展[J].浙江大学学报(医学版),2008,37(3):315-320.
[15]张壮,骆江云,黄静,等.白三烯D_4体外诱导小胶质细胞激活的作用[J].浙江大学学报(医学版),2013,42(3):253-260.
[16] Sowndhararajan K, Deepa P, Kim M, et al. Neuroprotective and Cognitive Enhancement Potentials of Baicalin: A Review[ J] . Brain Sci,2018,8( 6) :1 -24.
[17] Li F, Li W, Li X, et al. Geniposide attenuates inflammatory response by suppressing P2Y14 receptor and downstream ERK1/2 signaling pathway in oxygen and glucosedeprivation - induced brain microvascular endothelial cells[ J] . J Ethnopharmacol,2016,185:77 -86.

Last Update: 2019-05-29