[1] Shi Dandan, Wang Qian, Xie Pei, et al. Effects of Banxia Xiexin Decoction and Its Disassembled Prescriptions on Cytokines in Colonic Tissue of Rats with Ulcerative Colitis Induced by TNBS [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2018, (05): 114-118. [doi: DOI:10．13424/j．cnki．jsctcm．2018．05．034]
Effects of Banxia Xiexin Decoction and Its Disassembled Prescriptions on Cytokines in Colonic Tissue of Rats with Ulcerative Colitis Induced by TNBS
- Article number:
- 2096－1340(2018)04－0114－05
- Keywords:
- Banxia Xiexin Decoction; ulcerative colitis( UC) ; cytokines
- ZTFLH:
- R574. 62
- Code:
- A
- Abstract:
- To observe the effect of Banxia Xiexin Decoction and its decomposed formula on the changes of cytokine content in the colon tissue of ulcerative colitis ( UC) rats，and to explore the mechanism of the effect of Banxia Xiexin Decoction and its disassembled prescription in the treatment of UC． Method: SD rats were used as the research object，and the animal model of ulcerative colitis was replicated by TNBS ethanol enema method． The contents of TNF －alpha，IL －1， IL －10，PGE2 and MPO in colonic tissue were detected by double antibody and sandwich enzyme － linked immunosorbent assay． Histopathological observation was carried out by HE staining． Result: Compared with the model group， the content of TNF － a， IL －1 and MPO in Banxia Xiexin Decoction group，cold and warm natured group ( pinellia ternata，dried ginger，scutellaria，rhizoma coptidis) ，pungent and warm natured group ( pinellia ternata，dried ginger) and sweet nursing group ( radix ginseng，radix glycyrrhizae，jujube) and bitter descending group ( scutellaria baicalensis，rhizoma coptidis) all decreased ( P ＜ 0． 05) ． Compared with Banxia Xiexin Decoction group，the content of TNF － a，IL －1 in cold and warm natured group，pungent and warm natured group，sweet nursing group and bitter descending group were relatively high ( P 0． 05) ， the content of MPO in pungent and warm natured group and sweet nursing group were relatively high ( P ＜0． 05) ． Compared with cold and warm natured group， the content of TNF － a，IL －1 in in pungent and warm natured group and bitter descending group were relatively high ( P ＜0． 05) ， the content of MPO in pungent and warm natured group was relatively high ( P ＜0． 05) ． Conclusion: The effect of Banxia Xiexin Decoction and its decomposed formula on TNF － α，IL －1，MPO and PGE2 in colon tissue of UC rats was the most obvious in Banxia Xiexin Decoction group， followed by cold and warm natured group，pungent and warm natured group，bitter descending group and sweet nursing group． Banxia Xiexin Decoction and its decomposed formula on the pathological changes of colon tissue in UC rats were most obvious in the Banxia Xiexin Decoction group and cold and warm natured group， followed by bitter descending group and pungent and warm natured group．
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Memo
基金项目:陕西省教育厅科研基金项目( 15JK1201)
通讯作者:王乾( 1984 －) ，男，主治医师，硕士，肝胆普外科疾病的临床与基础研究。E － mail:shidan0324@ 163． com收稿日期:2017 －11 －27 编辑:文颖娟