[1]王莉刘妍如周宁余莎熊恬.生精补血方干预运动性疲劳组方配伍合理性及作用机制探索[J].陕西中医药大学学报,2024,(03):082-93.[doi:10.13424/j.cnki.jsctcm.2024.03.012]
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生精补血方干预运动性疲劳组方配伍合理性及作用机制探索()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2024年03期
页码:
082-93
栏目:
出版日期:
2024-05-20

文章信息/Info

文章编号:
2096-1340(2024)03-0082-12
作者:
王莉刘妍如周宁余莎熊恬
陕西中医药大学,陕西 咸阳 712046
关键词:
关键词:生精补血方运动性疲劳慢性疲劳综合征网络药理学
分类号:
R289
DOI:
10.13424/j.cnki.jsctcm.2024.03.012
文献标志码:
A
摘要:
摘要:目的 基于网络药理学分析方法,探索生精补血方治疗运动性疲劳的组方配伍合理性,阐述其作用机制。方法 本研究以生精补血方抗运动性疲劳的组方合理性为切入点,首先从中药成分数据库中筛选生精补血方有效活性成分,并找到对应的治疗靶点。然后与从疾病数据库中提取的疾病靶点进行共有靶点交集分析,建立“组方药材-活性成分-运动性疲劳-靶点”关联靶点集合,进一步将关联靶点集合进行蛋白互作网络构建。进一步采用CytoScape软件分别构建生精补血方和“君臣佐使”组方的“组方药材-活性成分-运动性疲劳-靶点”关联网络,筛选其中的关键成分、靶点。最后对筛选的关键靶点进行生物信息学与代谢通路富集分析,完成整方和组方的作用机制预测及配伍合理性评价。结果 从各中药成分数据库中收集得到122个成分,符合ADME药动学参数标准的活性成分37个,关联靶点417个。从疾病数据库及GEO数据库中得到与运动性疲劳(慢性疲劳综合征)相关的靶点1894个。对上述成分和疾病相关靶点取交集得到靶点45个,经蛋白质互作分析后,构建生精补血方“组方药材-活性成分-运动性疲劳-靶点”关联网络。从关联网络中提取到14个关键成分,27个关键作用靶点。生物信息学结果显示,生精补血方中的“君”“臣”“佐”药在分别作用于运动性疲劳相关的IL-17信号通路、PI3K-Akt信号通路、Th17细胞分化等信号通路的同时,又共同作用于JAK-STAT信号通路以维持骨骼肌功能。结论 生精补血方主要通过TNF、IL6、NR3C1、ALB、IL1B等多靶点和PI3K-Akt信号通路和细胞因子受体相互作用通路等途径按照“君、臣、佐、使”配伍规律发挥了对运动性疲劳的作用。

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备注/Memo

备注/Memo:
基金项目:陕西省教育厅专项科研计划项目(2013JK0813);陕西省体育局常规课题(13003)
更新日期/Last Update: 2024-05-20