[1]但林蔚高天慧张东东黄文丽姜祎王海芳宋小妹王薇 李玉泽.竹根七总皂苷的提取纯化工艺及抗炎活性研究[J].陕西中医药大学学报,2024,(03):034-42.[doi:10.13424/j.cnki.jsctcm.2024.03.004]
 ZHOU QianYI YaqiaoCHEN YanjieLIU LinSONG ZhenyanDENG YihuiYANG Xin.Interpreting the Application of Zhongjing’s Time Medicine Based on “Ready to Solve” of Six Meridian Diseases[J].Journal of Shaanxi University of Traditional Chinese Medicine,2024,(03):034-42.[doi:10.13424/j.cnki.jsctcm.2024.03.004]
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竹根七总皂苷的提取纯化工艺及抗炎活性研究()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2024年03期
页码:
034-42
栏目:
出版日期:
2024-05-20

文章信息/Info

Title:
Interpreting the Application of Zhongjing’s Time Medicine Based on “Ready to Solve” of Six Meridian Diseases
文章编号:
2096-1340(2024)03-0034-09
作者:
但林蔚1高天慧1张东东12黄文丽12姜祎12王海芳3宋小妹12王薇12 李玉泽12
1.陕西中医药大学,陕西 咸阳 712046;
2.陕西省太白七药研究与应用重点研究室,陕西 咸阳 712046;
3.陕西中医药大学陕西省中西医结合心血管病防治重点实验室,陕西 咸阳 712046
Author(s):
ZHOU Qian1YI Yaqiao1CHEN Yanjie1LIU Lin2SONG Zhenyan1DENG Yihui1YANG Xin3
1.Hunan University of Chinese Medicine,Changsha 410208,China;
2.The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;
3.Ethnic Traditional Chinese Medicine Hospital of Xiangxi Tujia and Miao Autonomous Prefecture,Hunan Jishou 433100,China
关键词:
关键词:竹根七总皂苷提取工艺纯化工艺RAW264.7细胞
Keywords:
Key words:Treatise on Cold DamageTime medicine“Ready to Solve”of six meridian diseasesZHANG ZhongjingLiterature research
分类号:
R284.2;R285.5
DOI:
10.13424/j.cnki.jsctcm.2024.03.004
文献标志码:
A
摘要:
摘要:目的 对提取与纯化竹根七中总皂苷的工艺条件进行优化与提升,初步探索验证其抗炎活性。方法 以总皂苷含量为评价指标,采用正交实验设计,通过回流提取法考察提取温度、时间、溶剂浓度及料液比对总皂苷含量的影响,进行统计分析;利用大孔树脂的吸附纯化作用,探究吸附和解吸附过程中不同条件对总皂苷纯度的影响,分析得出最佳纯化工艺;以RAW264.7细胞上清IL-1β、IL-6、TNF-α水平为指标,通过CCK-8法筛选最佳给药浓度及ELISA试剂盒检测细胞上清炎症因子水平,考察竹根七总皂苷抗炎活性。结果 最优回流提取工艺为乙醇浓度70%,料液比1∶40,温度80 ℃,提取时间30 min,竹根七总皂苷最终得率为9.74%;在最优提取方法下,探究竹根七皂苷纯化富集工艺,选用D101大孔树脂,当上样量为1 BV,吸附时间12 h,洗脱剂为50%乙醇-水,洗脱体积5 BV时,纯化后总皂苷纯度达到70%以上,由此得出其最佳纯化工艺;纯化后的总皂苷对RAW264.7细胞炎症具有抑制作用,其最低质量浓度为8 μg·mL-1。结论 本实验所得工艺能较好的富集竹根七总皂苷,该方法简单易行稳定可靠;所得竹根七皂苷具有较好的抗炎活性。
Abstract:
Abstract:The theory of time medicine related to traditional Chinese medicine is a discipline guided by traditional Chinese medicine theory,which studies the correspondence between human physiological pathology and natural time rhythms.This theory originated from the Inner Canon of Huangdi and was inherited and developed by ZHANG Zhongjing.It is mainly reflected in the content of the six meridian diseases in the Treatise on Cold Damage,such as the “Ready to Solve”,and elaborates on the time medicine theory.Through the exploration and research of relevant medical history literature,this study focuses on the understanding of the human body’s time rhythm,the occurrence and development rhythm of diseases,and the timing of disease treatment by Zhongjing and later medical practitioners.By integrating the development of modern and contemporary time medicine theory,this study aims to enrich the theory of time medicine in traditional Chinese medicine and guide clinical practice.

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备注/Memo

备注/Memo:
基金项目:陕西省教育厅课题(22JK0344); 陕西中医药大学校级课题(2021GP27);陕西中医药大学创新团队项目(2023-CXTD-05)
更新日期/Last Update: 2024-05-20