[1]任燕?? 罗春丽 蒋雯文 黄明进 谢丹.β-桉叶醇体外对 IEC-6 细胞增殖和迁移的影响[J].陕西中医药大学学报,2021,44(03):080-85.[doi:10. 13424 / j. cnki. jsctcm. 2021. 03. 018]
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β-桉叶醇体外对 IEC-6 细胞增殖和迁移的影响
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
44
期数:
2021年03期
页码:
080-85
栏目:
出版日期:
2021-05-30

文章信息/Info

文章编号:
2096-1340(2021)03-0080-06
作者:
任燕?? 罗春丽 蒋雯文 黄明进 谢丹
贵州大学,贵州 贵阳 550025
关键词:
β-桉叶醇小肠隐窝细胞鸟氨酸脱氢酶钙离子瞬时受体 1 型电位通道
分类号:
R28
DOI:
10. 13424 / j. cnki. jsctcm. 2021. 03. 018
文献标志码:
A
摘要:
目的 通过体外大鼠小肠隐窝细胞( Intestinal epithelial cells,IEC-6)实验观察 β-桉叶醇对于小肠细 胞修复功能的影响及可能机制。 方法 采用不同浓度(0、10、20、40、80、160mol·L - 1 )β-桉叶醇处理 IEC-6 细胞, 用 MTT 法检测 24、48、72 h 细胞增殖情况。 采用酶联免疫吸附试验(ELISA 法)检测细胞鸟氨酸脱羧酶(ODC) 浓度,采用细胞划痕法测定 β-桉叶醇对细胞迁移的影响,用流式细胞仪检测细胞内游离钙[Ca2 + ] cyt 浓度和细 胞周期情况,并用 RT-PCR 和蛋白印迹法( Western Blot) 分析 TRPC1 蛋白表达。 结果 与空白对照组相比, 10mol·L - 1β-桉叶醇对 IEC-6 细胞增殖有显著影响(p < 0. 01),且划痕实验结果表明 β-桉叶醇能快速刺激损伤 后 IEC - 6 细胞迁移,对细胞迁移的促进作用明显;ELISA 实验结果表明,β-桉叶醇组细胞中 ODC 浓度高于空白 组(P < 0. 05);流式细胞实验表明 β-桉叶醇组细胞内 Ca2 + 浓度高于空白组,有显著性差异( p < 0. 05)。 细胞周 期分布较空白组在 G1 期和 S 期有显著性差异(p < 0. 01);RT-PCR 和 Western blot 实验结果表明 β-桉叶醇组较 空白组能促进 TRPC1 蛋白表达。 结论 β-桉叶醇促进 IEC - 6 细胞增殖和迁移可能是通过增加 ODC、Ca2 + 、 [Ca2 + ]cyt 的浓度、促使 TRPC1 蛋白表达而增强 TRPC1 介导的 Ca2 + 内流,而 Ca2 + 在上皮细胞增殖和迁移中发 挥着重要作用

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备注/Memo

备注/Memo:
基金项目:贵州省科技厅联合基金项目(黔合科 LH 字(2017)7295)
更新日期/Last Update: 2021-06-08