[1]蔡佳 李漓 严晟 周小江 袁志鹰 许光明 **.原位微提-固相萃取-液相色谱法测定玄参中五种指标性成分[J].陕西中医药大学学报,2021,44(02):061-68.[doi:10.13424/j.cnki.jsctcm.2021.02.013]
 CAI Jia,LI Li,YAN Sheng,et al.Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(02):061-68.[doi:10.13424/j.cnki.jsctcm.2021.02.013]
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原位微提-固相萃取-液相色谱法测定玄参中五种指标性成分()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
44
期数:
2021年02期
页码:
061-68
栏目:
出版日期:
2021-03-26

文章信息/Info

Title:
Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography
文章编号:
2096-1340(2021)02-0061-08
作者:
蔡佳 1 李漓 1 严晟 1 周小江 12 袁志鹰 12 许光明 12**
1. 湖南中医药大学,湖南 长沙 410208;
2. 湖南省中药饮片标准化及功能工程技术研究中心,湖南 长沙 410208
Author(s):
CAI Jia 1 LI Li 1 YAN Sheng 1 ZHOU Xiao - jiang 12 YUAN Zhi - ying 12 XU Guang - ming 12
1. Hunan university of traditional Chinese medicine, Changsha 410208,China;
2. Hunan Provincial Research Center for standardization and functional engineering of Chinese herbal pieces,Changsha 410208, China
关键词:
原位微提固相萃取 - 液相色谱法玄参桃叶珊瑚苷哈巴苷安格洛苷 C哈巴俄苷肉桂酸
Keywords:
In situ microextraction Solid phase extraction liquid chromatography Radix Scrophulariae AucubinHarpagoside Angloride C Harpagoside Cinnamic acid
分类号:
R284
DOI:
10.13424/j.cnki.jsctcm.2021.02.013
文献标志码:
A
摘要:
目的建立原位微提-固相萃取-液相色谱法(原位微提-SP-HPLC法),应用于玄参中桃叶珊瑚苷?安格洛苷 C?哈巴苷?肉桂酸?哈巴俄苷的快速提取?净化?测定? 方法 注溶剂于玄参药材微孔中,快速提取后使用 C18 固相萃取法净化,采用液相色谱法测定指标性成分? 结果 采用 7. 5g 的玄参块,在 35℃?微孔大小1mm ×1mm 时,用比例为 50% 的甲醇溶液 1mL 进行固相萃取提取,玄参各指标性成分分离度高?峰形较好?结论 原位微提 - 固相萃取 - 液相色谱法以原位预处理操作简便?用样极少?取样快?试剂用量少且净化效果良好的优点,实现了快速提取?净化?测定玄参指标性成分,为快速测定玄参指标性成分含量提供了新方案。
Abstract:
Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.

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备注/Memo

备注/Memo:
基金项目:国自然青年基金项目(81703824);国家中医药管理局标准化建设项目(ZYBZH - Y - HUN -24);湖南省重点研发计划项目(2017NK2261)
通讯作者:许光明,博士,副教授,硕士生导师 E - mail: 1052262329@ qq. Com
更新日期/Last Update: 2021-03-29