[1]惠建荣,吕瑶,李永峰,等.通利枢机针刺法对脑缺血大鼠 Notch1的影响及机制[J].陕西中医药大学学报,2020,(01):062-68.[doi:10.13424/j.cnki.jsctcm.2020.01.017]
 Hui Jianrong,Lv Yao,Li Yongfeng,et al.Effect and Mechanism of Pivot Function Promoting Acupuncture on Notch1 in Rats with Cerebral Ischemia[J].Journal of Shaanxi University of Traditional Chinese Medicine,2020,(01):062-68.[doi:10.13424/j.cnki.jsctcm.2020.01.017]
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通利枢机针刺法对脑缺血大鼠 Notch1的影响及机制()
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《陕西中医药大学学报》[ISSN: 2096-1340/CN: 61-1501/R]

卷:
期数:
2020年01期
页码:
062-68
栏目:
出版日期:
2020-01-30

文章信息/Info

Title:
Effect and Mechanism of Pivot Function Promoting Acupuncture on Notch1 in Rats with Cerebral Ischemia
文章编号:
2096-1340(2020)01-0062-07
作者:
惠建荣吕瑶李永峰张瑞
1.陕西中医药大学第二附属医院,陕西 咸阳 712000;2.铜川市人民医院,陕西 铜川 727031;3.安康市中医医院,陕西 安康 725000
Author(s):
Hui Jianrong 1 Lv Yao 2 Li Yongfeng 1 Zhang Rui 3
1. The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang China, 712000; 2. Peoples Hospital of Tongchuan, Tongchuan China, 727031; 3. Ankang TCM Hospital, Ankang China, 725000
关键词:
利枢针刺法缺血性脑中风Notch信号通路Notch1蛋白
Keywords:
Pivot Function Promoting Acupuncture ischemic cerebral apoplexy Notch signaling pathwayNotch1 protein
分类号:
R289.51
DOI:
10.13424/j.cnki.jsctcm.2020.01.017
文献标志码:
A
摘要:
目的 探讨通利枢机针刺法对脑缺血大鼠神经功能修复作用及对 Notch1蛋白表达的影响及机制。方法 60只雄性 SD大鼠随机选取 10只作为假手术组,余 50只线栓法 MCAO造模后将造模成功者随机分为模型对照组、利枢针刺组、普通针刺组及西药灌胃组,予以各组造模后 1天、3天、7天、14天时间点进行神经功能评分;14d后处死大鼠部分测定脑梗死体积百分比,部分检测 Notch1蛋白的表达水平。结果 术后 7天及 14d时,利枢针刺法神经缺损情况较其他组明显改善(P<0.01或 P<0.05);与其他组相比较,利枢针刺法可明显降低脑梗死体积(P<0.01);与其他组相比较,利枢针刺法可促进脑缺血后 Notch1蛋白的表达,其效果优于普通针刺法及西药灌胃法(P<0.05或 P<0.01)。结论 利枢针刺法能有效改善脑缺血大鼠神经功能评分和脑梗死体积,并促进 Notch1蛋白的表达,其作用机制可能与 Notch信号通路相关。
Abstract:
Objective To investigate the effect of Pivot Function Promoting Acupuncture on neurological function repair and Notch 1 protein expression in rats with cerebral ischemia. Methods 10 out of the 60 male SD rats were randomly selected as the sham operation group. The remaining 50 rats were randomly divided into model control group, Lishu Acupuncture( Pivot Function Promoting Acupuncture) group, ordinary acupuncture group and Western medicine lavage group after MCAO modeling by suture method. Neurological function scores were obtained at the first day, third days, seventh day, and fourteenth day after modeling. The rats were sacrificed after 14 days to determine the percentage of cerebral infarction volume and the expression of Notch1 protein partially. Results On the 7th and 14th day after operation, compared with other groups, the nerve defect by Lishu Acupuncture was significantly improved ( P < 0. 01) ; Lishu Acupuncturecould significantly reduce the volume of cerebral infarction ( P < 0. 01) ; it could promote the expression of Notch – 1 protein after cerebral ischemia, and its effect was better than that of common acupuncture and Western medicine of lavage ( P < 0. 05 or P < 0. 01) . Conclusion Lishu Acupuncture can effectively improve the neurological function score and cerebral infarction volume in rats with cerebral ischemia, and promote the expression of Notch1 protein. The mechanism may be related to the Notch signaling pathway.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2018YFC1704606);陕西省科技厅科技计划项目(2016JQ8020);陕西省教育厅科 技计划项目 (14JK1211);咸阳市科技局项目 (2017k-02-101);陕西省中医药管理局科研项目 (LCPT103) 作者简介:惠建荣(1978- ),女,副教授,研究方向:中医、针灸防治疑难疾病的机理与临床研究。E-mail: 157600958@qq.com
更新日期/Last Update: 2020-01-15