[1]刘霜月 许潇莹 张玉莲 艾双春.电针治疗对 MCAO模型大鼠缺血半暗带 GDNF表达的影响[J].陕西中医药大学学报,2019,(05):058-63.[doi:10.13424/j.cnki.jsctcm.2019.05.014]
 Liu Shuangyue Xu Xiaoying Zhang Yulian Ai Shuangchun.Effect of Electroacupuncture on Expression of GDNF in Ischemic Penumbra of MCAO Model Rats[J].Journal of Shaanxi University of Traditional Chinese Medicine,2019,(05):058-63.[doi:10.13424/j.cnki.jsctcm.2019.05.014]
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电针治疗对 MCAO模型大鼠缺血半暗带 GDNF表达的影响 ()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2019年05期
页码:
058-63
栏目:
针推研究
出版日期:
2019-09-16

文章信息/Info

Title:
Effect of Electroacupuncture on Expression of GDNF in Ischemic Penumbra of MCAO Model Rats
文章编号:
2096-1340(2019)05-0058-06
作者:
刘霜月1 许潇莹1 张玉莲1 艾双春2
成都中医药大学
Author(s):
Liu Shuangyue 1 Xu Xiaoying 1 Zhang Yulian 1 Ai Shuangchun 2
1. Chengdu University of Chinese Medicine, Chengdu China, 610075; 2. Mianyang TCM Hospital, Mianyang China, 621000
关键词:
电针脑卒中缺血再灌注损伤GDNFmRNANF-κB
Keywords:
electro - acupuncture stroke ischemia reperfusion injury GDNFmRNA NF-κB
分类号:
R245.31+9
DOI:
10.13424/j.cnki.jsctcm.2019.05.014
文献标志码:
A
摘要:
目的 观察电针“水沟”“内关”“三阴交”“委中”穴,对脑缺血再灌注大鼠缺血半暗带区胶质细胞源 性神经营养因子(glialcelllinederivedneurotrophicfactor,GDNF)表达的影响,探讨其减轻脑缺血再灌注损伤的 作用机制。方法 将 120只清洁级成年雄性 Sprague-Dawley(SD)大鼠,随机分为空白组、假手术组、模型组、电 针组和抑制剂组 5组,每组 24只。参照 Longa改良线栓法,制备大脑中动脉栓塞(middlecerebralarteryocclu sion,MCAO)模型。电针组以电针刺激大鼠“水沟”“内关”“三阴交”“委中”穴,每天 1次,连续治疗 28天。抑制 剂组以 NF-κB抑制剂 PDTC进行大鼠腹腔注射后(120mg/kg),再行电针刺激,每天 1次,连续治疗 28天。应 用实时荧光定量聚合酶链式反应(polymerasechainreaction,PCR),检测大鼠脑组织缺血半暗带区 GDNF的 mR NA表达。结果 与模型组相比,电针组 GDNFmRNA表达在治疗后第 3天时下降,在第 7、14、28天时表达上升, 差异有统计学意义(P<0.05);NF-κB抑制剂组 GDNFmRNA表达在第 3、7天时下降(P<0.05),在第 14、28天 时差异不明显无统计学意义(P>0.05)。与电针组比较,抑制剂组在治疗后 4个观察时间点 GDNFmRNA表达 均下调明显,差异有统计学意义(P<0.05)。结论 电针治疗能上调 GDNFmRNA的表达,对缺血再灌注损伤大 鼠起到脑保护作用,其作用机制可能与 NF-κB信号通路的活跃性有
Abstract:
To observe the effect of electro - acupuncture at Shuigou, Neiguan, Sanyinjiao and Weizhong points on the expression of glial cell line derived neurotrophic factor ( GDNF) in ischemic penumbra of rats with cerebral ischemia - reperfusion, and to explore its mechanism of reducing cerebral ischemia - reperfusion injury. Methods 120 adult male Sprague - Dawley ( SD) rats of clean grade were randomly divided into five groups: blank group, sham operation group, model group, electro - acupuncture group and inhibitor group, with 24 rats in each group. The middle cerebral artery occlusion ( MCAO) model was established by using Longa’ s modified thread embolization method. In the electro - acupuncture group, rats were stimulated by electro - acupuncture at Shuigou, Neiguan, Sanyinjiao and Weizhong points once a day for 28 days. The inhibitor group was intraperitoneally injected with NF - κB inhibitor PDTC ( 120 mg/kg) , and then electro - acupuncture was stimulated once a day for 28 days. Real - time fluorescence quantitative polymerase chain reaction ( PCR) was used to detect the mRNA expression of GDNF in the ischemic penumbra of rat brain.Results Compared with the model group, the expression of GDNF mRNA in the electroacupuncture group decreased on the 3rd day after treatment, and the expression increased on the 7th, 14th and 28th days, the difference was statistically significant ( P <0. 05) . The expression of GDNF in NF - kappa B inhibitor group decreased on the 3rd and 7th day ( P < 0.05) , but there was no significant difference on the 14th and 28th day ( P > 0. 05) . The expression of GDNF mRNA was significantly down - regulated in the inhibitor group at the four observation time points after treatment, and the difference was statistically significant ( P <0. 05) . Conclusion Electroacupuncture can up - regulate the expression of GDNFmRNA and protect the rats from ischemia - reperfusion. The mechanism may be related to the activity of NF - kB signaling pathway.

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备注/Memo

备注/Memo:
 基金项目:绵阳市科技局资助项目(2015yy037)
更新日期/Last Update: 2019-09-15