[1]张丽 赵锐.葛根素对 ２ 型糖尿病小鼠心梗后心肌能量代谢的影响[J].陕西中医药大学学报,2019,(02):046-49.[doi:10.13424/j.cnki.jsctcm.2019.02.016]
　Zhang Li,Zhao Rui.Effect of Puerarin on Myocardial Energy Metabolism in Type 2 Diabetic Mice after Myocardial Infarction[J].Journal of Shaanxi University of Traditional Chinese Medicine,2019,(02):046-49.[doi:10.13424/j.cnki.jsctcm.2019.02.016]
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葛根素对 ２ 型糖尿病小鼠心梗后心肌能量代谢的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2019年02期
页码:
046-49
栏目:
实验研究
出版日期:
2019-03-10

文章信息/Info

Title:
Effect of Puerarin on Myocardial Energy Metabolism in Type 2 Diabetic Mice after Myocardial Infarction
文章编号:
2096-1340(2019)02-0046-04
作者:
张丽 赵锐
陕西中医药大学第二附属医院， 陕西 咸阳 712000
Author(s):
Zhang Li;  Zhao Rui
The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang China, 712000
关键词:
糖尿病小鼠; 葛根素; 糖尿病?冠脉结扎; ＧＬＵＴ４;  ＣＤ３６
Keywords:
diabetic mice;  puerarin;  diabetic mice with coronary ligation;  GLUT4;  CD36
分类号:
R587.2
DOI:
10.13424/j.cnki.jsctcm.2019.02.016
文献标志码:
A
摘要:
目的 探讨葛根素对 ２ 型糖尿病小鼠心梗后心肌能量代谢的影响。 方法 建立糖尿病?心梗小鼠模型，将动物分为糖尿病假心梗组（ｓｈａｍｅ）；糖尿病心梗组（ＭＩ）；葛根素组（ＭＩ ＋ ＰＵＥ），第 ３ 组小鼠腹腔注射葛根素 １． ３ｍＬ·ｋｇ － １·ｄ － １，治疗 ４ 周，统计 ３ 组小鼠的生存率。 提取各组小鼠心肌组织总蛋白和膜蛋白，取各组蛋白样品 ２０μｇ，用 Ｗｅｓｔｅｒｎ ｂｌｏｔ 方法检测心肌细胞 ＧＬＵＴ４ 和 ＣＤ３６ 蛋白的表达。 结果 结扎后的四周，ＭＩ 组超过一半的小鼠死亡。 ＭＩ ＋ ＰＵＥ 组生存率较 ＭＩ 组显著提高 （ Ｐ ＝ ０． ０４８）；糖尿病? 冠脉结扎小鼠，总 ＧＬＵＴ４ 和 ｍＧＬＵＴ４ 蛋白均增多；总 ＣＤ３６ 以及 ｍＣＤ３６ 蛋白均减少；与 ＭＩ 组比较，ＭＩ ＋ ＰＵＥ 组的 ＧＬＵＴ４ 和 ｍＧＬＵＴ４ 增多而ＣＤ３６ 和 ｍＣＤ３６ 减少。 结论 葛根素通过调控能量代谢来改善糖尿病?冠脉结扎小鼠的心脏功能，其潜在机制可能是葛根素提高了 ＧＬＵＴ４ 的转运和表达，同时减弱了 ＣＤ３６ 的转运和表达。
Abstract:
Objective To investigate the effect of puerarin on myocardial energy metabolism in type 2 diabetic mice after myocardial infarction. Method The mice model of diabetic myocardial infarction were established and divided into diabetic pseudomyocardial infarction group ( shame) , diabetic myocardial infarction group ( MI) , puerarin group ( MI + PUE) . The mice in group 3 were injected puerarin 1. 3 mL? kg-1 ? d -1 intraperitoneally for 4 weeks, and the survival rates of the three groups were counted. Total and membrane proteins were extracted from myocardial tissues of mice in each group. The expression of GLUT4 and CD36 proteins in myocardial cells was detected by Western blot. Result Four weeks after ligation, more than half of the MI mice died. The survival rate of MI + PUE group was significantly higher than that of MI group ( P =0. 048) ; the total GLUT4 and mGLUT4 protein increased in diabetic mice with coronary ligation; the total CD36 and mCD36 protein decreased; compared with MI group, the GLUT4 and mGLUT4 increased in MI + PUE group, while the CD36 and mGLUT36 decreased. Conclusion Puerarin improves cardiac function in diabetic mice with coronary artery ligation by regulating energy metabolism. Potential mechanism is that puerarin increases the transportand expression of GLUT4 and decreases the transport and expression of CD36.

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备注/Memo

备注/Memo:
基金项目：陕西省中医药管理局资助项目（ＺＹＭＳ０１５）
更新日期/Last Update: 2019-03-15