[1]朱青 李明 王佳音 吴太鼎 陈宗海 朱垚 陈龙菊.姜黄素经SLC7A11调控骨肉瘤细胞铁死亡机制初探[J].陕西中医药大学学报,2024,(02):017-21.[doi:10.13424/j.cnki.jsctcm.2024.02.004]
　ZHU QingLI Ming WANG JiayinWUTaidingCHEN Zonghai ZHU YaoCHEN Longju.Preliminary Study on the Mechanism of Curcumin Regulating Iron Death in Osteosarcoma Cells through SLC7A11[J].Journal of Shaanxi University of Traditional Chinese Medicine,2024,(02):017-21.[doi:10.13424/j.cnki.jsctcm.2024.02.004]
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姜黄素经SLC7A11调控骨肉瘤细胞铁死亡机制初探()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2024年02期
页码:
017-21
栏目:
出版日期:
2024-03-20

文章信息/Info

Title:
Preliminary Study on the Mechanism of Curcumin Regulating Iron Death in Osteosarcoma Cells through SLC7A11
文章编号:
2096-1340(2024)02-0017-05
作者:
朱青1 李明1 王佳音1 吴太鼎1 陈宗海1 朱垚2 陈龙菊1
1.湖北民族大学医学部，湖北 恩施 445000;
2.南京中医药大学，江苏 南京 210029
Author(s):
ZHU Qing1LI Ming1 WANG Jiayin1WUTaiding1CHEN Zonghai1 ZHU Yao1CHEN Longju1
1.Medical Department of Hubei University for Nationalities,Hubei Enshi 445000,China;
2.Nanjing University of Chinese Medicine,Nanjing 210029,China
关键词:
关键词：姜黄素; 溶质载体家族7成员11; 骨肉瘤; 铁死亡
Keywords:
Key words： Curcumin; 7 members of the solute carrier family 11; Osteosarcoma; Ferroptosis
分类号:
B21,R221
DOI:
10.13424/j.cnki.jsctcm.2024.02.004
文献标志码:
A
摘要:
摘 要：目的 初步探讨姜黄素（curcumin，Cur）经SLC7A11调探对骨肉瘤U-2 OS细胞铁死亡的影响。方法 CCK-8法检测0、5、10、20、40、80 μmol·L-1的Cur对U-2 OS细胞抑制的影响，同时在倒置显微镜下观察细胞形态学的变化。其次，将体外培养U-2 OS细胞随机分为Con组（0 μmol·L-1）、Fer-1组（4 μmol·L-1）、Cur组（40 μmol·L-1）、Cur+Fer-1组（40 μmol·L-1+4 μmol·L-1）用Western blot法检测SLC7A11的表达。结果 Cur在浓度为10～80 μmol·L-1时均对U-2 OS细胞显著性抑制。进一步研究表明，Cur可以显著诱导U-2 OS细胞铁死亡，下调SLC7A11的表达。结论 Cur可以诱导U-2 OS细胞铁死亡，其机制可能是通过下调SLC7A11实现铁死亡。
Abstract:
Abstract： Objective To investigate the effect of curcumin (Cur) on ferroptosis in osteosarcoma U-2 OS cells based on SLC7A11.Methods The CCK-8 method was used to detect the effect of Cur at 0,5,10,20,40,and 80 μmol·L-1 on the inhibition of U2-OS cells,and the morphological changes of cells were observed under an inverted microscope.Secondly,U-2 OS cells cultured in vitro were randomly divided into Con group (0 μmol·L-1),Fer-1 group (4 μmol·L-1),Cur group (40 μmol·L-1),and Cur+Fer-1 group (40 μmol·L-1+4 μmol·L-1),and the expression of SLC7A11 was detected using Western blot.Results Cur significantly inhibited U-2 OS cells at concentrations of 10-80 μmol·L-1.Further research has shown that Cur can significantly induce ferroptosis in U-2 OS cells and downregulate the expression of SLC7A11.Conclusion Cur can induce ferroptosis in U-2 OS cells,and its mechanism may be achieved by downregulating SLC7A11 to achieve ferroptosis.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（81260192）
更新日期/Last Update: 2024-03-29