[1]郑佳荟,李欢,赵重博,等.黄芩汤、葛根芩连汤对溃疡性结肠炎 小鼠肠道菌群的影响[J].陕西中医药大学学报,2023,(02):053-62.[doi:10.13424/j.cnki.jsctcm.2023.02.008]
　ZHENG Jiahui LI Huan ZHAO Chongbo GUO Dongyan WANG Jing.Effect of Huangqin Decoction and Gegen Qinlian Decoction onIntestinal Flora in Mice with Ulcerative Colitis[J].Journal of Shaanxi University of Traditional Chinese Medicine,2023,(02):053-62.[doi:10.13424/j.cnki.jsctcm.2023.02.008]
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黄芩汤、葛根芩连汤对溃疡性结肠炎 小鼠肠道菌群的影响
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2023年02期
页码:
053-62
栏目:
出版日期:
2023-05-13

文章信息/Info

Title:
Effect of Huangqin Decoction and Gegen Qinlian Decoction on Intestinal Flora in Mice with Ulcerative Colitis
文章编号:
2096-1340(2023)02-0053-10
作者:
郑佳荟1; 2;  李欢1; 2;  赵重博1; 2;  郭东艳1; 2
1.陕西中医药大学秦药特色资源研究与开发国家重点实验室（培育）/ 陕西省中药基础与新药研究重点实验室，陕西 咸阳 712046；
2.陕西中医药大学，陕西 咸阳 712046
Author(s):
ZHENG Jiahui1; 2 LI Huan1; 2 ZHAO Chongbo1; 2 GUO Dongyan1; 2 WANG Jing1; 2
1.State Key Laboratory for Research and Development of Characteristic Resources of Chinese Medicine of Shaanxi University of Chinese Medicine (cultivation)/Shaanxi Key Laboratory for Basic and New Drug Research of Chinese Medicine,Shaanxi Xianyang 712046,China;
2.Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China
关键词:
关键词：黄芩汤; 葛根芩连汤; 葡聚糖硫酸钠; 肠道菌群
Keywords:
Key words: Huangqin Decoction; Gegen Qinlian Decoction; Dextran sodium sulfate; Intestinal flora
分类号:
R285.5
DOI:
10.13424/j.cnki.jsctcm.2023.02.008
文献标志码:
A
摘要:
摘 要： 目的 探讨黄芩汤、葛根芩连汤对溃疡性结肠炎（UC）小鼠肠道菌群的调节作用。方法 采用3%葡聚糖硫酸钠（DSS）水溶液自由饮用7 d建立UC小鼠模型，同时连续7 d灌服不同剂量黄芩汤和葛根芩连汤，通过观察各组小鼠的体质量、疾病活动指数评分（DAI）、结肠长度及组织病理学来评价两种复方对UC小鼠的疗效，采用16S rDNA高通量测序探究黄芩汤、葛根芩连汤对UC小鼠肠道菌群的影响。结果 与模型组比较，黄芩汤中、高剂量组与葛根芩连汤中、高剂量组均显著改善了小鼠结肠组织病理学、体质量、DAI评分、结肠长度。在肠道菌群方面,两种复方均能调节门水平上的拟杆菌门（Bacteroidota）、厚壁杆菌门（Firmicutes）的相对丰度；在属水平上均能降低有害菌大肠杆菌志贺菌属（Escherichia-Shigella）的相对丰度，其中，黄芩汤在增加有益菌乳杆菌属（Lactobacillus）、毛螺菌属NK4A136（Lachnospiraceae_NK4A136_group）、肠杆菌属（Enterorhabdus）的相对丰度上优于葛根芩连汤；葛根芩连汤在增加有益菌布劳特氏菌属（Blautia）、降低有害菌变形菌门（Proteobacteria）的相对丰度上优于黄芩汤。结论 黄芩汤中剂量在改善体质量、DAI评分、结肠长度方面优于葛根芩连汤不同剂量组，且两种复方可能通过调节肠道菌群平衡从而减轻DSS诱导的UC，其中，黄芩汤在调节有益菌的数目上优于葛根芩连汤。
Abstract:
Abstract： Objective To investigate the regulatory effects of Huangqin Decoction and Gegen Qinlian Decoction on intestinal flora in mice with ulcerative colitis (UC).Methods The UC mice model was established by drinking 3% dextran sodium sulfate (DSS) aqueous solution freely for 7 days.At the same time,different doses of Huangqin Decoction and Gegen Qinlian Decoction were administered for 7 days.The therapeutic effects of the two compounds on UC mice were evaluated by observing the body mass,disease activity index score (DAI),colon length and histopathology of mice in each group.The 16S rDNA high-throughput sequencing was used to explore the effects of Huangqin Decoction Effect of Gegen Qinlian Decoction on intestinal flora of UC mice.Results Compared with the model group,the middle and high dose groups of Huangqin Decoction and the middle and high dose groups of Gegen Qinlian Decoction significantly improved the histopathology,body mass,DAI score and colon length of mice colon.In terms of intestinal flora,both compounds can regulate the relative abundance of Bacteroidota and Firmicutes at the gate level;At the genus level,it can reduce the relative abundance of harmful bacteria Escherichia-Shigella.Among them,Huangqin Decoction is superior to Gegen Qinlian Decoction in increasing the relative abundance of Lactobacillus,Lachnospiraceae_NK4A136_group and Enterorabdus;Gegen Qinlian Decoction is superior to Huangqin Decoction in increasing the relative abundance of beneficial bacteria Blautia and reducing the harmful bacteria Proteobacteria.Conclusion The medium dose of Huangqin Decoction is superior to Gegen Qinlian Decoction in improving body mass,DAI score and colon length,and the two compounds may alleviate the UC induced by DSS by regulating the balance of intestinal flora,among which,Huangqin Decoction is superior to Gegen Qinlian Decoction in regulating the number of beneficial bacteria.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（81903892）
更新日期/Last Update: 2023-03-27