[1]张攀攀 林静瑜 周凡 许金森 程佑民.足三里穴位注射对胃溃疡大鼠Gsh-Px、EGF、Gas及Nrf2、GSTm1基因的影响[J].陕西中医药大学学报,2023,(01):087-93.[doi:2096-1340(2023)01-0087-07]
　ZHANG Panpan LIN Jingyu ZHOU Fan XU Jinshen CHENG Youmin.Effects of Zusanli Point Injection on Gsh-Px,EGF,Gas,Nrf2,GSTm1 Genes in Rats with Gastric Ulcer[J].Journal of Shaanxi University of Traditional Chinese Medicine,2023,(01):087-93.[doi:2096-1340(2023)01-0087-07]
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足三里穴位注射对胃溃疡大鼠Gsh-Px、EGF、Gas及Nrf2、GSTm1基因的影响
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2023年01期
页码:
087-93
栏目:
实验研究
出版日期:
2023-01-20

文章信息/Info

Title:
Effects of Zusanli Point Injection on Gsh-Px,EGF,Gas, Nrf2,GSTm1 Genes in Rats with Gastric Ulcer
文章编号:
10.13424/j.cnki.jsctcm.2023.01.016
作者:
张攀攀1 林静瑜2 周凡2 许金森2 程佑民2
1.福建中医药大学，福建 福州 3501082;
2.福建省中医药科学院，福建 福州 350003
Author(s):
ZHANG Panpan1 LIN Jingyu2 ZHOU Fan2 XU Jinshen2 CHENG Youmin2
1.Fujian University of Chinese Medicine，Fuzhou 3501082,China；
2.Fujian College of Chinese Medicine，Fuzhou 350003,China
关键词:
关键词：足三里; 电针预处理; 穴位注射; 胃溃疡; Gas; EGF; GSTm1
Keywords:
Key words:Zusanli; Electroacupuncture pretreatment; Point injection; Gastric ulcer; Gas;  EGF;  GSTm1
分类号:
R245.9+5
DOI:
2096-1340(2023)01-0087-07
文献标志码:
A
摘要:
摘 要：目的 探讨足三里防治非甾体抗炎药胃溃疡的作用机理。方法 SD大鼠按随机数字法分为正常组、模型组、足三里组，每组8只。模型组、足三里组采用腹腔注射吲哚美辛制备胃溃疡模型：足三里组采用电针预处理结合穴位注射NS（生理盐水）干预，干预方式：造模前6 d，连续电针大鼠双侧足三里20 min,1次/d，并于第7 d造模后60 min，于双侧足三里注射NS 0.5 mL·kg-1；各组大鼠于造模后7 h麻醉，取血清、胃，计算胃黏膜损伤分数、HE染色观察胃黏膜病理形态。检测谷胱甘肽过氧化物酶（GSHPx）活力，表皮生长因子（EGF）、胃泌素（Gas）含量，以及胃Nrf2、GSTm1基因表达。结果 与模型组比，足三里组胃黏膜大体外观及病理形态损伤明显改善，胃黏膜损伤分数显著降低（P<0.01）；与正常组比，模型组大鼠胃组织GSHPx活力，胃组织和血清中EGF含量下降（P均<0.01）、Gas升高（P<0.05、P<0.01）；与模型组比，足三里组大鼠胃组织GSHPx活力、胃组织和血清EGF含量均升高（P<0.05、P<0.01）、Gas均明显下降（P<0.01）；与正常组比，模型组大鼠胃组织GSTm1 mRNA表达下降（P<0.05）；与模型组比，足三里组大鼠胃组织GSTm1 mRNA表达上升（P<0.05）。结论 足三里保护吲哚美辛实验性胃溃疡大鼠胃黏膜的作用，与提高胃组织GSHPx活力、促进EGF分泌，降低胃泌素分泌，促进胃组织GSTm1 mRNA表达有关，针灸可能在抗氧化、促进胃黏膜修复、降低胃酸分泌方面发挥防治胃溃疡作用。
Abstract:
Abstract：Objective To investigate the mechanism of Zusanli in preventing and treating gastric ulcer with NSAID.Methods SD rats were randomly divided into normal group，model group and Zusanli group with 8 rats in each group.The model group and Zusanli group were treated with intraperitoneal injection of indomethacin to prepare gastric ulcer model.The Zusanli group was treated with electroacupuncture pretreatment combined with acupoint injection of NS (normal saline).The intervention mode was:6 days before modeling，the rats were continuously electroacupunctured at Zusanli on both sides for 20 minutes，once a day，and at 60 minutes after modeling on the 7th day，NS 0.5 mL·kg-1 was injected at Zusanli on both sides；The rats in each group were anesthetized 7 hours after modeling，the serum and stomach were taken，and the gastric mucosa injury fraction was calculated，and the pathological morphology of gastric mucosa was observed by HE staining.The activity of glutathione peroxidase (GSH Px)，the content of epidermal growth factor (EGF) and gastrin (Gas)，and the expression of Nrf2 and GSTm1 genes in stomach were detected.Results Compared with the model group，the gross appearance and pathological morphology of gastric mucosa in Zusanli group were significantly improved，and the score of gastric mucosa damage was significantly reduced (P＜0.01)；Compared with the normal group，the activity of GSH Px in gastric tissue，the content of EGF in gastric tissue and serum in the model group decreased (P＜0.01)，and the content of gas increased (P＜0.05，P＜0.01)；Compared with the model group，the activity of GSH Px in stomach tissue，the content of EGF in stomach tissue and serum of rats in Zusanli group increased (P＜0.05，P＜0.01)，and the content of gas decreased significantly (P＜0.01)；Compared with the normal group，the expression of GSTm1 mRNA in the stomach tissue of the model group decreased (P＜0.05)；Compared with the model group，the expression of GSTm1 mRNA in stomach tissue of rats in Zusanli group increased (P＜0.05).Conclusion Zusanli protects the gastric mucosa of indomethacin induced experimental gastric ulcer rats，which is related to the increase of GSH Px activity in gastric tissue，the promotion of EGF secretion，the reduction of gastrin secretion，and the promotion of GSTm1 mRNA expression in gastric tissue.Acupuncture and moxibustion may play a role in preventing and treating gastric ulcer in terms of antioxidation，the promotion of gastric mucosal repair，and the reduction of gastric acid secretion.Abstract：Objective To investigate the mechanism of Zusanli in preventing and treating gastric ulcer with NSAID.Methods SD rats were randomly divided into normal group，model group and Zusanli group with 8 rats in each group.The model group and Zusanli group were treated with intraperitoneal injection of indomethacin to prepare gastric ulcer model.The Zusanli group was treated with electroacupuncture pretreatment combined with acupoint injection of NS (normal saline).The intervention mode was:6 days before modeling，the rats were continuously electroacupunctured at Zusanli on both sides for 20 minutes，once a day，and at 60 minutes after modeling on the 7th day，NS 0.5 mL·kg-1 was injected at Zusanli on both sides；The rats in each group were anesthetized 7 hours after modeling，the serum and stomach were taken，and the gastric mucosa injury fraction was calculated，and the pathological morphology of gastric mucosa was observed by HE staining.The activity of glutathione peroxidase (GSH Px)，the content of epidermal growth factor (EGF) and gastrin (Gas)，and the expression of Nrf2 and GSTm1 genes in stomach were detected.Results Compared with the model group，the gross appearance and pathological morphology of gastric mucosa in Zusanli group were significantly improved，and the score of gastric mucosa damage was significantly reduced (P＜0.01)；Compared with the normal group，the activity of GSH Px in gastric tissue，the content of EGF in gastric tissue and serum in the model group decreased (P＜0.01)，and the content of gas increased (P＜0.05，P＜0.01)；Compared with the model group，the activity of GSH Px in stomach tissue，the content of EGF in stomach tissue and serum of rats in Zusanli group increased (P＜0.05，P＜0.01)，and the content of gas decreased significantly (P＜0.01)；Compared with the normal group，the expression of GSTm1 mRNA in the stomach tissue of the model group decreased (P＜0.05)；Compared with the model group，the expression of GSTm1 mRNA in stomach tissue of rats in Zusanli group increased (P＜0.05).Conclusion Zusanli protects the gastric mucosa of indomethacin induced experimental gastric ulcer rats，which is related to the increase of GSH Px activity in gastric tissue，the promotion of EGF secretion，the reduction of gastrin secretion，and the promotion of GSTm1 mRNA expression in gastric tissue.Acupuncture and moxibustion may play a role in preventing and treating gastric ulcer in terms of antioxidation，the promotion of gastric mucosal repair，and the reduction of gastric acid secretion.

参考文献/References:

［1］程佑民,林静瑜,周凡,等.预针刺结合穴位注射对胃溃疡大鼠胃黏膜的损伤修复作用及机制探讨［J］.中国医药科学,2021,11(3):20-24.
［2］于婧妍,李静.NSAIDs相关性胃病研究进展［J］.锦州医科大学学报,2018,39(1):104-107.
［3］梁丽珠,杨良俊,樊湘珍.针灸足三里的分子生物学效应机制研究［J］.国际中医中药杂志,2020,42(11):1125-1129.
［4］向杜炼. 电针治疗对AD小鼠行为学及海马区突触相关蛋白PSD-95、SYP表达的影响［D］.北京：北京中医药大学,2019.
［5］李忠仁.实验针灸学［M］.2版.北京：中国中医药出版社，2007.
［6］刘密,雷毅军,潘思安,等.艾灸预处理对大鼠应激性胃黏膜损伤中保护因子的调节作用［J］.中华中医药学刊,2015,33(9):2068-2071.
［7］Sabiu S,Garuba T,Sunmonu T,et al.Indomethacininduced gastric ulceration in rats:protective roles of Spondias mombin and Ficus exasperata［J］.Toxicology Reports,2015,2:261-267.
［8］徐叔云，卞如濂，陈修．药理实验方法学［M］．2版．北京：人民卫生出版社，1994:1157-1160．
［9］肖韦，许昂，季晖. 胃溃疡药理模型的研究进展［J］. 药学与临床研究，2016，24（2）：145-150.
［10］Guth PH,Aures D,Paulsen G.Topical aspirin plus HCl gastric lesions in the rat:cytoprotective effect of prostaglandin,cimetidine,and probanthine［J］.Gastroenterology,1979,76(1):88-93.
［11］周华,周美启. 针灸治未病特点及作用机制研究进展［C］∥2019中国针灸学会年会暨40周年回顾论文集，湖北武汉，中国针灸学会，2019：908-911
［12］高璇,鲁海,高霄英,等.“预针刺”的现代作用机制研究进展［J］.针灸临床杂志，2020,36(8):87-90.
［13］华金双,邵素菊,王培育,等.逆针灸对过敏性哮喘大鼠血清IL-2含量及肺组织GATA-3蛋白表达的影响［J］.中医研究,2020,33(5):63-67.
［14］邓伊健,曾丽莹,曲姗姗,等.针灸治未病浅谈［J］.河南中医,2018,38(4):505-507.
［15］Dengiz GO,Odabasoglu F,Halici Z,et al.Gastroprotective and antioxidant effects of montelukast on indomethacin-induced gastric ulcer in rats［J］.Journal of Pharmacological Sciences,2007,105(1):94-102.
［16］Lanas A,GarcíaRodríguez LA,Arroyo MT,et al.Risk of upper gastrointestinal ulcer bleeding associated with selective cyclo-oxygenase-2 inhibitors,traditional non-aspirin non-steroidal anti-inflammatory drugs,aspirin and combinations［J］.Gut,2006,55(12):17311738.
［17］谢慧臣,刘芬,杨强.加味四逆散对慢性心理性应激胃溃疡模型大鼠胃黏膜的保护作用［J］.中国实验方剂学杂志,2012,18(19):252256.
［18］马鸿雁.应激与非应激状态下急性酒精中毒大鼠洗胃及其效果的研究［D］.长春:吉林大学,2006.
［19］胡明曦,张栩,陈畅.细胞氧化还原调控与衰老 ［J］.生物化学与生物物理进展,2014,41(3):288-294.
［20］Tanaka J,Yuda Y.Lipid peroxidation in gastric mucosal lesions induced by indomethacin in rat［J］.Biological & Pharmaceutical Bulletin,1996,19(5):716-720.
［21］Tarnawski A,Ahluwalia A,Jones MK.Gastric cytoprotection beyond prostaglandins:cellular and molecular mechanisms of gastroprotective and ulcer healing actions of antacids［J］.Current Pharmaceutical Design,2013,19(1):126-132.
［22］Tonelli C,Chio IIC,Tuveson DA.Transcriptional regulation by Nrf2［J］.Antioxidants & Redox Signaling,2018,29(17):1727-1745.
［23］杨志国,鲍春花.谷胱甘肽转硫酶 M1 和 T1 基因多态性与肝癌易感性关系的研究［J］.中国医学工程,2020,28(10):26-28.
［24］Chang VC,Cotterchio M,Bondy SJ,et al.Iron intake,oxidative stressrelated genes and breast cancer risk［J］.International Journal of Cancer,2020,147(5):1354-1373.
［25］李会庆，周涛，温培娥，等. CYP1A1 和GSTM1与胃癌、萎缩性胃炎和胃溃疡等易感性的研究［J］,中国肿瘤，2002,11(1):25-28.

相似文献/References:

[1]张攀攀 林静瑜 周凡 许金森 程佑民.足三里穴位注射对胃溃疡大鼠Gsh-Px、EGF、Gas及Nrf2、GSTm1基因的影响[J].陕西中医药大学学报,2023,(01):087.[doi:2096-1340(2023)01-0087-07]
　ZHANG Panpan LIN Jingyu ZHOU Fan XU Jinshen CHENG Youmin.Effects of Zusanli Point Injection on Gsh-Px,EGF,Gas,Nrf2,GSTm1 Genes in Rats with Gastric Ulcer[J].Journal of Shaanxi University of Traditional Chinese Medicine,2023,(01):087.[doi:2096-1340(2023)01-0087-07]

备注/Memo

备注/Memo:
基金项目：福建省自然科学基金资助项目（2018J01853，2021J01918）；福建省卫计委中青年人才骨干项目（2018-ZQN-73）
更新日期/Last Update: 2023-02-20