[1]蔡佳 李漓 严晟 周小江 袁志鹰 许光明 **.原位微提-固相萃取-液相色谱法测定玄参中五种指标性成分[J].陕西中医药大学学报,2021,44(02):061-68.[doi:10.13424/j.cnki.jsctcm.2021.02.013]
　CAI Jia,LI Li,YAN Sheng,et al.Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography[J].Journal of Shaanxi University of Traditional Chinese Medicine,2021,44(02):061-68.[doi:10.13424/j.cnki.jsctcm.2021.02.013]
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原位微提-固相萃取-液相色谱法测定玄参中五种指标性成分()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
44
期数:
2021年02期
页码:
061-68
栏目:
出版日期:
2021-03-20

文章信息/Info

Title:
Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography
文章编号:
2096-1340(2021)02-0061-08
作者:
蔡佳 1 李漓 1 严晟 1 周小江 1; 2 袁志鹰 1; 2 许光明 1; 2**
1. 湖南中医药大学,湖南 长沙 410208;
2. 湖南省中药饮片标准化及功能工程技术研究中心,湖南 长沙 410208
Author(s):
CAI Jia 1 ;  LI Li 1 ;  YAN Sheng 1 ;  ZHOU Xiao - jiang 1; 2 ;  YUAN Zhi - ying 1; 2 ;  XU Guang - ming 1; 2
1. Hunan university of traditional Chinese medicine, Changsha 410208,China;
2. Hunan Provincial Research Center for standardization and functional engineering of Chinese herbal pieces,Changsha 410208, China
关键词:
原位微提; 固相萃取 - 液相色谱法; 玄参; 桃叶珊瑚苷; 哈巴苷; 安格洛苷 C; 哈巴俄苷; 肉桂酸
Keywords:
In situ microextraction;  Solid phase extraction liquid chromatography;  Radix Scrophulariae;  Aucubin; Harpagoside;  Angloride C;  Harpagoside;  Cinnamic acid
分类号:
R284
DOI:
10.13424/j.cnki.jsctcm.2021.02.013
文献标志码:
A
摘要:
目的建立原位微提-固相萃取-液相色谱法(原位微提-SP-HPLC法),应用于玄参中桃叶珊瑚苷?安格洛苷 C?哈巴苷?肉桂酸?哈巴俄苷的快速提取?净化?测定? 方法 注溶剂于玄参药材微孔中,快速提取后使用 C18 固相萃取法净化,采用液相色谱法测定指标性成分? 结果 采用 7. 5g 的玄参块,在 35℃?微孔大小1mm ×1mm 时,用比例为 50% 的甲醇溶液 1mL 进行固相萃取提取,玄参各指标性成分分离度高?峰形较好?结论 原位微提 - 固相萃取 - 液相色谱法以原位预处理操作简便?用样极少?取样快?试剂用量少且净化效果良好的优点,实现了快速提取?净化?测定玄参指标性成分,为快速测定玄参指标性成分含量提供了新方案。
Abstract:
Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.

参考文献/References:

[1]国家药典委员会. 中华人民共和国药典[M]. 一部. 北京:中国医药科技出版社,2015:17.
[2]李杰,詹琤琤,段时振,等. 中药玄参研究概况[J]. 湖北中医杂志,2013,35(8):73 -75.
[3]许福泉,许旭东,陈士林. 玄参化学成分及药理活性研究进展[J]. 中国现代中药,2013,15(9):752 -759.
[4]张海霞,朱彭龄. 固相萃取[J]. 分析化学,2000,28(9):1172 -1180.
[5]吴芳华. 固相萃取新技术研究进展[J]. 分析测试技术与仪器,2012,21(2):114 -120.
[6]徐刚,史茗歌,吴明红,等. 固相微萃取的原理及应用[J]. 上海大学学报(自然科学版),2013,17(4):368-373.
[7]王蕾,郭丽冰. 固相萃取形式应用概况[J]. 河南中医学院学报,2007,42(4):86 -88.
[8]刘祺文,熊远福,王锦,等. 分子印迹固相萃取技术研究新进展[J]. 环境科技,2015,28(5):63 -67.[9]刘承伟,毕志明,祝艳斐,等. 玄参中 4 种主要活性成分的 HPLC 定量分析[J]. 中国药学杂志,2007,55(21):1614 -1616.
[10]翟有朋,张宗祥. 加速溶剂 - 固相萃取 - 高效液相色谱法测定废气中多环芳烃[J]. 环境科技,2018,31(2):65 -69.
[11]汪开银,熊晓辉,游京晶,等. 辣椒制品中苏丹红Ⅰ快速检测的方法研究[J]. 生物加工过程,2018,16(2):63-68.
[12]王静哲,刘震,马立满,等. 基于 UPLC - Q - TOF MS 分析加工炮制对玄参化学成分的影响[J]. 质谱学报,2016,37(1):1 -9.
[13]徐姗姗,杜伟锋,葛卫红,等. 近红外光谱法快速分析玄参药材中毛蕊花糖苷含量[J]. 安徽中医学院学报,2013,32(3):81 -84.
[14]喻欢欢,钟猛,丁锐,等. 一测多评法测定玄参中 7 种有效成分的含量[J]. 中国中药杂志,2017,42(14):2719 -2724.
[15]戴瑞,刘宁,王磊,等. 微波辅助法快速提取苹果皮膳食纤维[J]. 河南工业大学学报(自然科学版),2017,38(3):77 -82,109.
[16] Dawidowicz A L, Dybowski M P. Simple SPE – GC method for anethole determination in human serum[J].Journal of Separation Science, 2014, 37(4): 393 -397.[17]Deger A B,Gremm T J,Frimme F H. Problems and Solu-tions in Pesticide Analysis Using Solid ‐ phase Extraction(SPE) and Gas Chromatography Ion ‐ trap Mass Spec-trometry Detection (GC ‐ MS) [J]. Clean Soil Air Wa-ter, 2000,28(6):292 -299.

备注/Memo

备注/Memo:
基金项目:国自然青年基金项目(81703824);国家中医药管理局标准化建设项目(ZYBZH - Y - HUN -24);湖南省重点研发计划项目(2017NK2261)
通讯作者:许光明,博士,副教授,硕士生导师 E - mail: 1052262329@ qq. Com
更新日期/Last Update: 2021-03-29