[1]何 林,刘 寒,高原雪,等.5-LOX-CysLTs-CysLTsR表达规律及黄芩苷-栀子苷配伍对大鼠脑缺血抗炎作用机制研究[J].陕西中医药大学学报,2019,(03):057-63.[doi:10.13424/j.cnki.jsctcm.2019.03.017]
 He Lin Liu Han Gao Yuanxue Li Hao Hou Jianping Wang Bin.Study on the Expression Pattern of 5 - LOX - CysLTs – CysLTsR and the Anti - inflammatory Mechanism of Baicalin – Germanuin on Cerebral Ischemia in Rats[J].Journal of Shaanxi University of Traditional Chinese Medicine,2019,(03):057-63.[doi:10.13424/j.cnki.jsctcm.2019.03.017]
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5-LOX-CysLTs-CysLTsR表达规律及黄芩苷-栀子苷配伍对大鼠脑缺血抗炎作用机制研究()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2019年03期
页码:
057-63
栏目:
实验研究
出版日期:
2019-05-30

文章信息/Info

Title:
Study on the Expression Pattern of 5 - LOX - CysLTs – CysLTsR and the Anti - inflammatory Mechanism of Baicalin – Germanuin on Cerebral Ischemia in Rats
文章编号:
2096-1340(2019)03-0057-07
作者:
何 林刘 寒高原雪李 豪侯建平王 斌
陕西中医药大学,陕西咸阳712046
Author(s):
He Lin Liu Han Gao Yuanxue Li Hao Hou Jianping Wang Bin
Shaanxi University of Chinese Medicine, Xianyang China, 712046
关键词:
脑缺血黄芩苷栀子苷5-LOX/CysLTs/CysLT通路
Keywords:
cerebral ischemia Baicali Geniposide 5 - LOX/ CysLTs/ CysLT pathway
分类号:
R962A
DOI:
10.13424/j.cnki.jsctcm.2019.03.017
文献标志码:
A
摘要:
目的动态观察脑卒中大鼠脑组织半胱氨酰白三烯(CysLTs)活性及其5-脂氧酶(5-LOX)?半胱氨酰白三烯受体1(CysLTs1)?半胱氨酰白三烯受体2(CysLTs2)表达水平变化规律,探讨黄芩苷-栀子苷(7:3)配伍抗脑缺血炎性损伤机制?方法线栓法制备大脑中动脉堵塞模拟脑缺血模型,模型成功后随机分为空白对照组(Control)?模型组(pMCAO)?黄芩苷-栀子苷(7:3)30mg/kg?45mg/kg?60mg/kg组?齐留通45mg/kg组?孟鲁司特0.5mg/kg组,持续35d给药治疗,每隔7d,神经功能评分观察神经保护作用,酶联免疫吸附实验(ELISA)法测脑组织CysLTs含量;蛋白质印迹(WesternBlot)法检测脑组织5-LOX?CysLTs1?CysLTs2蛋白表达水平?结果与模型组相比,黄芩苷-栀子苷(7:3)30mg/kg?45mg/kg?60mg/kg组在脑缺血28d内可显著降低了CysLTs水平;WesternBlot结果显示,5-LOX?CysLTs1和CysLTs2蛋白表达明显降低;至恢复期35d,各蛋白表达水平已无显著性差异?结论黄芩苷-栀子苷(7:3)配伍可减轻MCAO大鼠的神经功能障碍,其作用机制可能与抑制5-LOX活性?降低CysLTs1和CysLTs2蛋白表达有关?
Abstract:
Objective To dynamicly observe cysteinyl leukotriene ( CysLTs) activity in brain tissue of stroke rats and its 5 - lipoxygenase ( 5 - LOX) , cysteinyl leukotriene receptor 1 ( CysLTs1) , cysteinyl The change of expression level of leukotriene receptor 2 ( CysLTs2) , and to investigate the mechanism of anti - cerebral ischemic injury induced by baicalin - germangoside ( 7: 3) . Methods The model of middle cerebral artery occlusion simulated cerebral ischemia was prepared by suture embolism. The model was then randomly divided into blank control group ( control) , model group ( pMCAO) , baicalin - geniposide ( 7: 3) 30 mg/ kg, 45 mg/ kg, 60 mg/ kg groups, ziliutong 45 mg/ kg group and montelukast 0. 5 mg/ kg group. The treatment lasted 35 days. Neuroprotective effect was observed by neurological function score every7 days. The content of CysLTs in brain tissue was measured by ELISA, and the expression levels of 5 - LOX, CysLTs1 and CysLTs2 in brain tissue were detected by Western Blot. Results Compared with the model group, baicalin - geniposide ( 7: 3) 30 mg/ kg, 45 mg/ kg and 60 mg/ kg groups could significantly reduce the level of CysLTs within 28 days after cerebral ischemia; Western Blot results showed that the expression of 5 - LOX, CysLTs1 and CysLTs2 protein was significantly decreased; 35 days after recovery, there was no significant difference in the expression levels of each protein. Conclusion Baicalin - geniposide ( 7: 3) combination can alleviate neurological dysfunction in MCAO rats, and its mechanism may be related to inhibiting 5 - LOX activity, reducing the expression of CysLTs1 and CysLTs2 protein.

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更新日期/Last Update: 2019-05-29