[1]王渊 刘智斌 牛文民 王强 刘思洋.嗅三针”对 AD小鼠海马 iNOS和磷酸化 tau蛋白表达的影响[J].陕西中医药大学学报,2018,(05):102-106.[doi:DOI:10.13424/j.cnki.jsctcm.2018.05.032]
 Wang Yuan,Liu Zhibin,Niu Wenmin,et al.Effect of "Sniffing Three Needles" on Expression of iNOS and Phosphorylated tau Protein in Hippocampus of AD Mice[J].Journal of Shaanxi University of Traditional Chinese Medicine,2018,(05):102-106.[doi:DOI:10.13424/j.cnki.jsctcm.2018.05.032]
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嗅三针”对 AD小鼠海马 iNOS和磷酸化 tau蛋白表达的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2018年05期
页码:
102-106
栏目:
实验研究
出版日期:
2018-09-20

文章信息/Info

Title:
Effect of "Sniffing Three Needles" on Expression of iNOS and Phosphorylated tau Protein in Hippocampus of AD Mice
文章编号:
2096-1340(2018)04-0102-05
作者:
王渊 刘智斌 牛文民 王强 刘思洋
1. 陕西中医药大学,陕西 咸阳 712046;2. 西安医学院,陕西 西安 710021
Author(s):
Wang Yuan1Liu Zhibin1Niu Wenmin1Wang Qiang1Liu Siyang2
1. Shaanxi University of Chinese Medicine,Xianyang China,712046; 2. Xi’an TCM Hospital,Xi’an China,710000
关键词:
阿尔茨海默病嗅三针 p - tau iNOS海马
Keywords:
alzheimer disease ( AD ) "sniffing three needles" p - tau iNOS hippocampus
分类号:
R749. 1 + 6
DOI:
DOI:10.13424/j.cnki.jsctcm.2018.05.032
文献标志码:
A
摘要:
目的 观察嗅三针对阿尔茨海默病( AD) 小鼠海马 iNOS 和磷酸化 tau 蛋白表达的影响,探讨其治疗 AD 的作用机制。方法 将40 只 AD 小鼠随机分为模型组、嗅三针组、嗅神经切断嗅三针组和盐酸多奈哌齐组, 10 只同窝阴性小鼠组成对照组。对照组和模型组于每日同一时段进行同种程度抓取,不干预,嗅三针组采用电针 刺激,选取“印堂”和双侧“迎香”三穴为干预穴位,留针20min,盐酸多奈哌齐组采用盐酸多奈哌齐灌胃,每周干 预5 次,间歇2 天,共干预4 周。干预结束后,运用 Western blot 法检测小鼠海马p - tau Thr181 蛋白表达,运用免 疫组化法检测小鼠海马 iNOS 蛋白表达。结果 模型组与对照组比较,小鼠海马 p - tau Thr181 和 iNOS 蛋白表达 显著增加( P <0. 01) ;与模型组比较,嗅三针电刺激和盐酸多奈哌齐灌胃能够显著降低小鼠海马 p - tau Thr181 和 iNOS 蛋白表达( P <0. 05) ,嗅神经切断嗅三针组对其无明显影响( P > 0. 05) ; 嗅三针组与盐酸多奈哌齐组比 较,无显著性差异( P >0. 05) 。结论 嗅三针可能基于嗅觉通路的完整性,发挥降低 AD 小鼠海马炎症因子 iNOS 和磷酸化 tau 蛋白的表达的作用。
Abstract:
To observe the effect of " sniffing three needles" on expression of iNOS and phosphorylated tau protein in hippocampus of AD mice and its mechanism of action in treating AD. Method: 40 AD mice were randomly divided into model group,sniffing three - needle group,three - needle olfactory nerve transection? group and donepezil hydrochloride group,and 10 littermates were used as control group. The control group and the model group were subjected to the same degree was kept for 20 minutes. The donepezil hydrochloride group was intragastrically administered with donepezil hydrochloride 5 times per week for a total of 4 weeks,with an interval of 2 days. After the intervention, the expression of p - tau Thr181 protein in hippocampus of mice was detected by Western blot,and the expression of iNOS protein in hippocampus of mice was detected by immunohistochemistry. Result: Compared with the control group,the expression of p - tau Thr181 and iNOS protein in the hippocampus of mice in the model group increased significantly ( P <0. 01) . Compared with the model group, the sniffing three - needle stimulation and donepezil perfusion could significantly reduce the expression of p - tau Thr181 and iNOS protein in the hippocampus of mice ( P < 0. 05) ,and three - needle olfactory nerve transection group had no significant effect on it ( P >0. 05) . There was no significant difference between the sniffing three - needle group anddonepezil hydrochloride group ( P >0. 05) . Conclusion: Based on the integrity of olfactory pathway,"sniffing three needles" may reduce the expression of inflammatory factor iNOS and phosphorylated tau protein in hippocampus of AD mice. of grasping at the same time every day without intervention,the sniffing three - needle group was stimulated by electroacupuncture on three intervention points of“Yintang”and“Yingxiang”in the two sides,and the needle

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备注/Memo

备注/Memo:

基金项目:国家自然科学基金项目( 81503667) ; 陕西中医药大学重点培育科研基金( 2015PY07)
通讯作者:王渊( 1980 -) ,男,博士,副教授,副主任医师,硕士生导师,研究方向:针灸推拿治疗神经退行性疾病和脊 柱病的基础与临床研究。E - mail:icherLes117@126. com.收稿日期:2018 -01 -25 编辑:文颖娟
更新日期/Last Update: 2019-12-10