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三味檀香汤散对心肌顿抑大鼠血浆及心肌组织中降钙素基因相关肽含量影响的研究()

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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:: 2017年04期

页码:: 090-94104

栏目:: 实验研究

出版日期:: 2017-07-05

文章信息/Info

Title:: The Influence of Sanwei Tanxiang Decoction Over CGＲP Contents in Myocardium Stunning Ｒats

文章编号:: 2096－1340(2017)04－0090－05

作者:: 李欣郑玉云杨玉梅; 1．包头医学院第一附属医院心内科，内蒙古包头 014010; 2．包头医学院心血管研究室，内蒙古包头 014010

Author(s):: Li Xin1 Zheng Yuyun1 Yang Yumei 2; 1． Department of Cardiology，the First Affiliated Hospital of Baotou Medical College，Baotou 014010，China; 2． Cardiovascular Ｒesearch Section of Baotou Medical College，Baotou 014010，China

关键词:: 三味檀香汤散; 心肌顿抑; 降钙素基因相关肽; ELISA

Keywords:: Sanwei Tanxiang Decoction; myocardium stunning; calcitonin gene related petide ( CGＲP) ; ELISA

分类号:: Ｒ2－031

DOI:: 10.13424/j.cnki.jsctcm.2017.04.031

文献标志码:: A

摘要:: 目的研究三味檀香汤散调节心肌顿抑大鼠血浆及心肌组织中降钙素基因相关肽( CGＲP) 的含量，为三味檀香汤散改善和保护心肌顿抑的作用和作用机制提供新的理论依据。方法雄性 SD 大鼠随机分成三味檀香汤散大、中、小剂量组( 简称大、中、小剂量组) 、硝苯地平对照组( 简称硝苯地平组) 、心肌顿抑模型组( 简称模型组) 和空白组 6 组。各给药组按照一定药量，连续灌胃给药 40 天，1 次/日，模型组和空白组以等容量的生理盐水灌胃，对各给药组及模型组制作大鼠心肌顿抑模型( 结扎冠状动脉左前降支 15m

Abstract:: Objective: To study whether Sanwei Tanxiang Decoction can the level of regulate calcitonin gene related petide ( CGＲP) in plasma and the myocardial tissue of myocardium stunning rats，provide new theoretical basis for its function in the improvement and protection of myocardium stunning and discover the acting mechanism． Method: Male SD rats were randomly divided into 6 groups: large，medium and small dose groups of Sanwei Tanxiang Decoction ( hereinafter referred to as the large，medium and small dose group) ，the nifedipine group，the model group and the blank group． The groups with medicine received the quantitative dosage and continuous lavage for 40 days，1 time /day; while the model blank groups only took physiological saline lavage of same capacity． Myocardium stunning rats were made in the medical and model groups ( 15 － minute ligation of left anterior descending coronary artery but just threading ligating lines through rats of blank group) ． Plasma and hearts were taken after 1 － hour reperfusion to determine CGＲP contents by ELISA and the received data were sorted out by mono factor analysis of variance，namely“P ＜ 0． 05”can 2017 年 7 月第 40 卷第 4 期陕西中医药大学学报 July． 2017 Vol． 40 No． 4 Journal of Shaanxi University of Chinese Medicine ·91· reveal the difference compared was statistically significant．Ｒesults: ① After 1 － hour reperfusion，the content of CGＲP in the plasma of the model group，compared with the blank group，increased and there was a significant statistical difference ( P ＜ 0． 01) ． In the comparison of the dosage groups and the nifedipine group with the model group，CGＲP levels in plasma remarkably rose，which had significant statistical differences ( P ＜ 0． 05) ． ②After 1 － hour reperfusion，the content of CGＲP in myocardial tissue of the model group，compared with the blank group，reduced and there was a significant statistical difference ( P ＜ 0． 01) ． In the comparison of the dosage groups and the nifedipine group with the model group，CGＲP levels in myocardial tissue remarkably elevated，which had significant statistical differences ( P ＜ 0． 01) ． Conclusion: ①Sanwei Tanxiang Decoction can visibly lift CGＲP levels in the plasma and myocardial tissue of myocardium stunning rats received 1 － hour repurfusion，which shows obvious quantity － effect relations． ②The elevating action of Sanwei Tanxiang Decoction may be one of mechanisms for protecting and improving myocardial stunning．

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备注/Memo:: 收稿日期:2017－03－14编辑:文颖娟

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