[1]施晓峰 黄晶晶 史 亚 丁志山 程东庆.半夏丛枝菌根真菌多样性研究[J].陕西中医药大学学报,2017,(03):075-81.[doi:10.13424/j.cnki.jsctcm.2017.03.026]
 Shi Xiaofeng Huang Jingjing Shi Ya Ding Zhishan Cheng Dongqing.On AMF Diversity of Pinellia Ternate[J].Journal of Shaanxi University of Traditional Chinese Medicine,2017,(03):075-81.[doi:10.13424/j.cnki.jsctcm.2017.03.026]
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半夏丛枝菌根真菌多样性研究()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2017年03期
页码:
075-81
栏目:
实验研究
出版日期:
2017-05-03

文章信息/Info

Title:
On AMF Diversity of Pinellia Ternate
文章编号:
2096-1340(2017)03-0075-07
作者:
施晓峰 黄晶晶 史 亚 丁志山 程东庆
浙江中医药大学,浙江 杭州 310053
Author(s):
Shi Xiaofeng Huang Jingjing Shi Ya Ding Zhishan Cheng Dongqing
Zhenjiang University of Chinese Medicine, Hangzhou 310053, China
关键词:
半夏 丛枝菌根真菌 孢子 侵染率 多样性
Keywords:
pinellia ternate AMF spores infection rate diversity
分类号:
R931.2
DOI:
10.13424/j.cnki.jsctcm.2017.03.026
文献标志码:
A
摘要:
目的 研究中药材半夏的丛枝菌根真菌( AMF) 的多样性。方法 采集浙江杭州( 虎跑、 下满觉陇、 富 阳) 、 贵州贵阳等地半夏及其根际土壤。块茎徒手切片、 根剪成 1 cm 的根段,经 10 % KOH 透明、 乳酸甘油酸性 品红染液染色、 乳酸甘油脱色后,镜下观察半夏植株内 AMF 的形态结构。湿筛倾析 - 蔗糖离心法分离半夏根际 土中 AMF 孢子并鉴定,分析各地半夏根际 AMF 孢子的多样性。结果 半夏的皮层组织细胞间及细胞内存在大 量菌丝、 泡囊,以及典型的丛枝结构。根际土壤中存在大量的 AM
Abstract:
To study the diversity of AMF ( Arbuscular mycorrhizal fungi) in pinellia ternate. Method: Pinellia ternate and rhizosphere soil were collected from Hangzhou in Zhenjiang Province ( including Hupao, Xiamanjuelong and Fuyang) and Guiyang in Guizhou Province. The tubers were cut into slices and roots made into 1cm long segments, which were colored and decolorized by lactated glycerid acid fuchsin to observe their AMF’ s morphological structure in plantlets of pinellia ternate. The AMF spores in rhizosphere soils were isolated by the method of wet screening and sucrose centrifugation and identified to analyze the diversity of the spores from different regions. Results: There were large quantities of hypha and vesicle between and inside cells of cortical tissues as well as the typical branched structure in pinellia ternate. A lot of AMF spores in rhizosphere soils were being existent. The identification showed that there were 20 kinds of pinellia ternate belonging to 3 genera, among which 16 kinds attributes to genus Glomus, 3 to genus Acaulospora and 1 to genus Scutellospora, taking up 80. 0% , 15. 0% and 5. 0% respectively. Discussion: AMF can be formed in all regions of pinellia ternate and AMF spores in rhizosphere soils are highly diverse, in which the genus Glomus are predominant bacterium.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-06-27编辑:文颖娟
基金项目:中医药行业科研专项子课题(201507002-1-11)
通讯作者:程东庆(1971-),副教授,硕士研究生导师,主要研究方向:药学、微生物学和免疫学研究。E-mail: B2006069@163.com
更新日期/Last Update: 2017-05-15