[1]李天浩 陈方园高小娟贾睿钟莉周莹朱晓娜.黄芪甲苷靶向miR-21调控PTEN/AKT/mTOR通路激活肺泡细胞自噬治疗肺纤维化[J].陕西中医药大学学报,2024,(06):102-109.[doi:10.13424/j.cnki.jsctcm.2024.06.017]
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黄芪甲苷靶向miR-21调控PTEN/AKT/mTOR通路激活肺泡细胞自噬治疗肺纤维化()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2024年06期
页码:
102-109
栏目:
出版日期:
2024-11-20

文章信息/Info

文章编号:
2096-1340(2024)06-0102-08
作者:
李天浩1 陈方园1高小娟1贾睿1钟莉2周莹2朱晓娜1
1.陕西中医药大学第二附属医院,陕西 西安 712000;
2.陕西中医药大学,陕西 咸阳 712000
关键词:
关键词:黄芪甲苷-IV自噬miR-21PTEN/AKT/mTOR信号通路特发性肺纤维化
分类号:
R932
DOI:
10.13424/j.cnki.jsctcm.2024.06.017
文献标志码:
A
摘要:
摘要:目的 探究黄芪甲苷-IV(Astragaloside IV,AS-IV)靶向miR-21调控PTEN/AKT/mTOR信号通路激活自噬,抑制特发性肺纤维化(Idiopathic Pulmonary Fibrosis,IPF)发展进程的作用机制。方法 通过气管注射10 mg·kg-1博莱霉素造模小鼠IPF,分别以10、20、40 mg·kg-1 AS-IV及5 mg·kg-1醋酸泼尼松连续治疗28 d。对肺样本进行伊红美蓝 ,马松染色,及透射电镜观察。实时荧光定量法(qRT-PCR)或蛋白免疫印迹法(Western blot)检测样本中miR-21、P62、Beclin-1、LC3B、PTEN、PI3K、p-AKT、AKT、p-mTOR、mTOR等的表达。结果 醋酸泼尼松及40 mg·kg-1 AS-IV可显著减少肺纤维组织增生与炎性细胞浸润,抑制嗜锇性板层小体变性,促进肺泡上皮细胞中自噬体的形成,显著上调LC3Ⅱ/LC3Ⅰ比例与Beclin-1、PTEN蛋白表达,下调P62、miR-21表达,并抑制mTOR与AKT的磷酸化。但AS-IV治疗对PI3K表达无明显影响。结论 AS-IV通过靶向miR-21调控PTEN/AKT/mTOR信号通路激活,从而激活肺泡细胞自噬逆转IPF。

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备注/Memo

备注/Memo:
基金项目:咸阳市二○二○年重点研发计划(2020k02-107)
更新日期/Last Update: 2024-11-21