[1]李英冯凯王文霞 严丽娟张国旗刘志艳周来来.血必净通过调节miR-145-5p/LOX信号通路对H2 O2 诱导的心肌细胞氧化应激及凋亡的影响[J].陕西中医药大学学报,2024,(05):097-102.[doi:10.13424/j.cnki.jsctcm.2024.05.016]
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血必净通过调节miR-145-5p/LOX信号通路对H2 O2 诱导的心肌细胞氧化应激及凋亡的影响()
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《陕西中医药大学学报》[ISSN:2096-1340/CN:61-1501/R]

卷:
期数:
2024年05期
页码:
097-102
栏目:
出版日期:
2024-09-09

文章信息/Info

文章编号:
2096-1340(2024)05-0097-06
作者:
李英1冯凯2王文霞1 严丽娟1张国旗1刘志艳1周来来1
1.滦南县医院，河北 唐山 063000;
2.无极县医院，河北 石家庄 050000
关键词:
关键词：血必净; 心肌细胞; 氧化应激; 细胞凋亡; miR-145-5p/LOX通路
分类号:
R285.5
DOI:
10.13424/j.cnki.jsctcm.2024.05.016
文献标志码:
A
摘要:
摘 要：目的 探讨血必净对H2O2诱导的心肌细胞氧化损伤及凋亡的影响及其分子机制。方法 培养H9C2细胞后将其随机分为对照组（Control）、模型组（Model）和血必净组（XBJ）3组，除Control组外，其余组的H9C2细胞用H2O2诱导构建氧化应激损伤细胞模型，然后XBJ组用12.5 g·L-1、25 g·L-1和50 g·L-1浓度的XBJ处理24 h。CCK-8法检测不同浓度的XBJ对细胞活力的影响；生化试剂盒检测肌酸激酶同工酶（CK-MB）、丙二醛（MDA）、超氧化物歧化酶（SOD）及还原型谷胱甘肽（GSH）的活性；流式检测H9C2细胞凋亡率；qRT-PCR检测miR-145-5p、LOX mRNA的表达；Western blot检测赖氨酰氧化酶蛋白（lysyloxidase，LOX）的表达；双荧光素酶实验检测miR-145-5p与LOX的靶向结合关系。结果 不同浓度XBJ处理后，细胞活性呈梯度性显著增高；与对照组相比，模型组细胞CK-MB、MDA的活性显著增加（P<0.01），SOD、GSH活性显著降低，细胞凋亡率显著提高，miR-145-5p表达水平显著降低，LOX水平显著增加（P<0.01）；经血必净处理后上述指标的水平得到有效改善（P<0.05）。结论 XBJ能通过提高抗氧化应激能力，抑制细胞凋亡并调控miR-145-5p/LOX信号通路保护H2O2诱导的心肌细胞损伤。

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备注/Memo

备注/Memo:
基金项目：河北省中医药管理局科研计划项目（2022213）
更新日期/Last Update: 2024-09-09