[1] JU Di LI MiHAN ManFANG BingyingYAN ShuguangAN Rong?LI Jingtao,.Effect of Yipi Yanggan Formula on the Activation of Hepatic Stellate Cells inRats with Pre Liver Cancer Lesions [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2024, (02): 83-88. [doi: 10.13424/j.cnki.jsctcm.2024.02.016]
Effect of Yipi Yanggan Formula on the Activation of Hepatic Stellate Cells inRats with Pre Liver Cancer Lesions
- Article number:
- 2096-1340(2024)02-0083-06
- Keywords:
- Key words： Precancerous lesions of liver cancer; Yipi Yanggan formula; Hepatic stellate cells; α-Smooth actin; Collagen deposition
- ZTFLH:
- R256.4
- Code:
- A
- Abstract:
- Abstract： Objective To explore the effects of Yipi Yanggan formula on the activation of hepatic stellate cells and collagen deposition in diethylnitrosamine (DEN) induced precancerous lesions of liver cancer.Methods 26 male Sprague Dawley rats were randomly divided into a blank group,a model group,a Yipi Yanggan formula group,and a Hugan tablet group.There were 5 rats in the blank group and 7 rats in each of the other 3 groups.Den was injected intraperitoneally to induce a model of liver cancer precancerous lesions,and was euthanized after 14 weeks of administration.Take liver tissue for observation and record changes in size,appearance,etc.,calculate liver weight ratio (liver index),observe pathological and morphological changes in rat liver tissue through HE staining,observe collagen deposition and expression in each group through Sirius red staining,detect the expression of alpha smooth muscle actin (α-SMA) through immunohistochemistry,and detect type Ⅰ collagen (Collagen Ⅰ) through real-time quantitative PCR (RT-PCR) To investigate the effect of Yipi Yanggan formula on the pre liver cancer lesion model in rats.Multiple inter group comparisons of quantitative data were conducted using one-way analysis of variance,and further pairwise comparisons were conducted using LSD-t test.Results Compared with the model group,the liver pathological morphology of the Yipi Yanggan formula group and the Hugan tablet group was significantly improved,and the liver index,Collagen I,and α-SMA expression were all reduced (P<0.05);Compared with the liver protection tablet group,the Yipi Yanggan formula group has a more significant protective effect on the liver,with significantly reduced liver index,Collagen I,and α-SMA expression (P<0.05).Conclusion Yipi Yanggan formula can effectively improve DEN induced precancerous lesions in rats,and its mechanism may be related to inhibiting hepatic stellate cell activation and reducing collagen deposition.
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Memo
基金项目：国家自然科学基金项目（81904192）；陕西省教育厅项目（19JK0244）；陕西中医药大学科学研究计划项目（2017QN15）；陕西中医药大学学科创新团队项目（2019-YS06）；陕西省自然科学基础研究计划青年项目(2022JQ-793，2022JQ-870)；陕西省中医药管理局项目（15-JC004）