[1] YU Kunpeng YU Yongduo,.Discussion on Mechanism of Hedyotis diffusa in Treatment of Ulcerative Colitis Based on Network Pharmacology [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (03): 87-96. [doi: 10.13424/j.cnki.jsctcm.2022.03.022]
Discussion on Mechanism of Hedyotis diffusa in Treatment of Ulcerative Colitis Based on Network Pharmacology
- Article number:
- 2096-1340(2022)03-0087-10
- Keywords:
- Key words： Ulcerative colitis; Hedyotis diffusa; Network pharmacology; Mechanism of action; Signal pathway
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract： Objective To analyze and discuss the potential therapeutic targets and mechanism of Hedyotis diffusa in the treatment of ulcerative colitis based on network pharmacological technology.Methods The effective components and corresponding targets of Hedyotis diffusa were screened by TCMSP.Using GeneCards，CTD，DisGeNET databases,the disease target proteins were queried with “ulcerative colitis” as the key word,and the disease-related targets were obtained.Use Venny 2.1,the common target of Hedyotis diffusa and ulcerative colitis was obtained as the prediction target,the PPI protein interaction network was constructed by using STRING database,and the drug active component target network diagram of Hedyotis diffusa in the treatment of ulcerative colitis was constructed by using Cytoscape.Cluster Profiler R package is used for GO function enrichment analysis and KEGG signal pathway analysis,and visual drawing is carried out.Results ①After screening and analysis,there were 7 effective compounds of Hedyotis diffusa,123 corresponding target genes and 17017 predicted target genes for the treatment of UC.There were 119 intersecting targets between drugs and ulcerative colitis.②The core genes in PPI network mainly include interleukin 6 (IL6),vascular endothelial growth factor A (VEGFA),tumor protein p53 (TP53),tumor necrosis factor (TNF),etc.③GO functional enrichment analysis showed that the biological process (BP) involved in the prediction target gene of Hedyotis diffusa to UC mainly included cell response to drugs,response to nutritional level,reactive oxygen species metabolism process,response to reactive oxygen species,etc;Cell components (CC) include membrane raft,membrane area,micromembrane area,synaptic membrane,components of postsynaptic membrane,etc;Molecular function (MF) mainly involves neurotransmitter receptor activity,G protein coupled amine receptor activity,serine hydrolase activity,serine endopeptidase activity and so on.KEGG pathway enrichment analysis showed that AGE-RAGE signal pathway,relaxin signal pathway,IL-17 signal pathway,TNF signal pathway,HIF-1 signal pathway and other major pathways in diabetes complications may be related to the treatment of ulcerative colitis with Hedyotis diffusa.Conclusion Hedyotis diffusa may play a role in the treatment of ulcerative colitis through multiple targets such as IL6,VEGFA,TP53,TNF,EDF,JUN,MAPK1 as well as AGE RAGE signal pathway,relaxin signal pathway,HIF-1 signal pathway,IL-17 signal pathway and PI3K-Akt signal pathway,which lays a theoretical foundation for further research.
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Memo
基金项目：辽宁省重点研发计划项目（2020JH2/10300062）