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[1] SU Yongjian DU Xin LIU Hongna ZHANG Xiaohua ZHANG Xianjuan,.Study on HPLC Fingerprint of Wuling Capsule [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (03): 46-50. [doi: 10.13424/j.cnki.jsctcm.2022.03.012]

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Study on HPLC Fingerprint of Wuling Capsule

Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R] Volumes & Issues: 2022 03 pagination: 46-50 Column: Publication date: 2022-05-26

Article number:: 2096-1340(2022)03-0046-05

Author(s):: SU Yongjian¹; 2 DU Xin¹ LIU Hongna¹ ZHANG Xiaohua² ZHANG Xianjuan¹; 1.Tsinghua Deren Xi'an Xingfu Pharmaceutical Co. ,Ltd. ,Xi'an 710043,China;
2.Shaanxi innovative Biotechnology Research Institute Co. ,Ltd. ,Xi'an 710075，China

Keywords:: Key words： Wuling capsule; HPLC; Fingerprint; Quality Control

ZTFLH:: R284.1

DOI:: 10.13424/j.cnki.jsctcm.2022.03.012

Code:: A

Abstract:: Abstract： Objective To establish a method for HPLC fingerprint of Wuling capsule.Methods Agilent ZORBAX SB-C18 column（250 mm × 4.6 mm，5 μm） was used;Mobile phase，acetonitrile（A）-0.1% phosphoric acid（B），gradient elution;Volume flow:0.8 mL·min-1;Columon temperature:30 ℃;Detection wavelength:210 nm;Injection volume:10 μL.Results The precision,repeatability and stability of the measured fingerprints were in line with the regulations.A total of 16 common peaks were determined in the HPLC fingerprints of 10 batches of samples,and 6 peaks were confirmed to be salvianolic acid B,saikosaponin a,schisandrin a,schisandrin a,tanshinone IIA and schisandrin B,with a similarity of >0.980.The sample fingerprints were in good agreement with the control fingerprints.Conclusion The method is stable and reliable,and the distribution difference of active components in Wuling capsule is clarified,which provides a scientific basis for the quality control of Wuling capsule.

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Memo

基金项目：陕西省创新生物技术研究院有限公司质量标准提高项目（2019XFJS001）

Last Update: 2022-05-30