[1] Tang Lanfang, Liu Ting, Zhong Jianan, et al. Effects of Cistanche on PI3K / AKT Pathway in the Medulla Oblongata of Rat Model of Parkinson’ s Disease [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2020, (02): 70-74. [doi: 10.13424/j.cnki.jsctcm.2020.02.018]
Effects of Cistanche on PI3K / AKT Pathway in the Medulla Oblongata of Rat Model of Parkinson’ s Disease
- Article number:
- 2096-1340(2020)02-0070-05
- ZTFLH:
- R741
- Code:
- A
- Abstract:
- Objective To study the effects of Cistanche on PI3K / AKT pathway in the medulla of rat model of Parkinson’ s Disease( PD) , and to explore the possible mechanism that cistanche improves the symptoms associated with Parkinson’s Disease. Methods Twenty - four SD rats successfully modeled were randomly divided into: model group, threegroups of different doses of hibiscus 40, 80 and 160 mg ? Kg-1 ? d -1 . and the normal group and sham operation group were set up, with 6 rats in each group. Cistanche or saline was administered to rats for 14 days. Western Blotting was used to detect the expression of AKT, PI3K and P - AKT, P - PI3K proteins in the rats’ medulla oblongata. Results Compared with the normal group, the expression of P - AKT and P - PI3K proteins in the medulla oblongata of the PD model group decreased ( P <0. 05) ; compared with the model group, the expression of P - AKT protein in the 80 and 160 mg? Kg-1 ? d -1 dose groups of the PD model rats was significantly increased ( P <0. 05) ; the expression of p - PI3K protein in three groups of 40, 80 and 160 mg ? Kg-1 ? d -1mg ? Kg-1 ?d-1was significantly increased, and there was a dose - dependent relationship ( P < 0. 05) . Conclusion Cistanche can increase the expression of P - AKT and P - PI3K proteins in the medulla oblongata and protect neurons.
References:
[1] Najiba M , Summers C M , Kokemuller R D , et al. Accelerated accumulation of retinal α – synuclein ( pSer129) and tau, neuroinflammation and autophagic dysregulation in a seeded mouse model of Parkinson’ s dis ease[ J] . Neurobiology of Disease, 2018: S096999611830 2043 - .
[2]钟佳宏,汪海涛,徐江平.α-突触核蛋白与帕金森病[J].神经药理学报,2017,7(2):51-52.
[3] Bradley F Boeve, Idiopathic REM sleep behaviour disorder in the development of Parkinson’ s disease; Lancet Neurol. 2013;12( 5) :469 -482,14
[4]宋艳玲,韩鹏敏,杜新春,等.抑制PI3K信号通路的抗肿瘤天然药物研究进展[J].现代药物与临床,2017,32(6):1156-1160.
[5] Hou L , Wang K , Zhang C , et al. Complement receptor 3 mediates NADPH oxidase activation and dopaminergic neurodegeneration through a Src - Erk - dependent pathway[ J] . Redox Biology, 2017, 14:250.
[6] Ascano M, Bodmer D, Kuruvilla R. Endocytic trafficking of neurotrophins in neural development. Trends Cell Biol. 2012 May;22( 5) :266 -73.
[7]彭芳,徐荣,徐常青,等.肉苁蓉药用及其食疗历史考证[J].中国药学杂志,2017,52(5):377-383.
[8]刘文滨.中药肉苁蓉有效化学成分对神经系统作用研究[J].内蒙古中医药,2014,33(25):118-120.
[9]覃威,魏维,许茜,等.肉苁蓉苷对C17.2神经干细胞增殖的影响[J].海峡药学,2015,27(12):263-265+296.
[10] Lan YL, Zhou JJ, et al . Uncariarhynchophylla Ameliorates Parkinson＇s Disease by Inhibiting HSP90 Expression: Insights from Quantitative Proteomics. Cell Physiol Biochem. 2018;47( 4) :1453 -1464.
[11]王莉,孙云燕,李冬冬,等.GOLPH3通过PI3K/Akt信号通路调控上皮性卵巢癌细胞增殖与凋亡的研究[J].现代妇产科进展,2018,27(9):648-651.
Memo
基金项目:福建省科技厅引导项目(2017Y0053);福建省中医药科研项目(2017FJZYLC501,2017FJZYLC201,2017FJZYLC202);福建省自然基金项目(2018J01859,2018J01860)? 通讯作者:林友宁,主任医师?E-mail:105393388@qq.com 通讯作者:肖绍坚,副主任医师?E-mail:1678725605@qq.com 通讯作者:蔡晶,教授?E-mail:caij1@163.com