[1] XIANG KemingWANG XiulianTANG QunbiyuHUANG Chao,.Analysis and Study on the Structural Composition and Functional Metabolic Changes of Gut Microbiota in Patients with Phlegm Dampness Syndrome Vertigo [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2024, (04): 23-30. [doi: 10.13424/j.cnki.jsctcm.2024.04.005]
Analysis and Study on the Structural Composition and Functional Metabolic Changes of Gut Microbiota in Patients with Phlegm Dampness Syndrome Vertigo
- Article number:
- 2096-1340(2024)04-0023-08
- Keywords:
- Key words： Intestinal microbiota; Dizziness; 16S microbial sequencing; Brain-gut axis; Phlegm dampness syndrome
- ZTFLH:
- R255.3
- Code:
- A
- Abstract:
- Abstract： Objective To analyze the differences in quantity,quality,and functional metabolism of gut microbiota in patients with phlegm dampness syndrome vertigo who seek medical treatment in hospitals and social health through secondgeneration highthroughput microbial sequencing based on 16S,in order to provide a certain basis for elucidating the pathogenesis of vertigo and improving treatment. Methods Fecal specimens were collected from 20 patients with phlegm dampness syndrome dizziness who received treatment at the Shanghe Community Health Service Center of Baoan District Traditional Chinese Medicine Hospital and the Traditional Chinese Medicine Department of Baoan District Peoples Hospital in Shenzhen (treatment group,20 cases). At the same time,fecal specimens were collected from healthy individuals who received control during the same period (control group,17 cases). Using secondgeneration highthroughput 16S rDNA microbial sequencing to detect and analyze the microbiome in feces. Results The abundance and evenness of the gut microbiota in the treatment group were lower than those in the control group,and the decreased abundance of the microbiota was mainly concentrated in Bacteroidetes,Bacteroidia,Bacteroidales,Bacteroidaceae,Prevotellaceae,Bacteroides,Prevotella,Megamonas,while the abundance of Proteobateria，Gammaproteobacteria，Actinobacteria，Enterobacteriales，Fusobacteriales，Bifidobacteriales，Ruminococcaceae，Enterobacteriaceae，Lachnospira of treatment group were relatively high compared to the control group.In addition,other bacterial genera with significantly increased abundance were found in the feces of patients in the study group. The β diversity of the research group is lower than that of the control group. The proportion of cysteine and methionine metabolism,glycine,serine and threonine metabolism,prokaryotic carbon fixation pathway,and riboflavin metabolism in the gut microbiota of patients in the treatment group were significantly lower than those in the control group (P<0.05). However,the proportion of chloroalkanes and their degradation,glycerol lipid metabolism,and other unclassified functional groups in the treatment group was significantly higher than that in the control group (P<0.05).Conclusion The number,structure,biodiversity,and metabolic function of gut microbiota in patients with phlegm dampness syndrome vertigo have undergone significant changes,which may be the intrinsic micro material basis for the occurrence of phlegm dampness syndrome and traditional Chinese medicine intervention.
References:
［1］SATOKARI R.Modulation of gut microbiota for health by current and next-generation probiotics［J］.Nutrients,2019,11(8):1921.
［2］BOSTANCIKLIO GˇLU M.The role of gut microbiota in pathogenesis of Alzheimer’s disease［J］.J Appl Microbiol,2019,127(4):954-967.
［3］张慧欣,第五永长,周源,安泰,等.补益脾胃调节肠道微生态防治阿尔茨海默病研究进展［J］.山东中医杂志,2021,40(4):425-428，439.
［4］张贤,齐祥,董忻悦,等.小肠细菌过度生长与慢性心力衰竭患者心功能的关系［J］.江苏大学学报(医学版),2021,31(3):251-256，276.
［5］于艳华.肠道微生物菌群对日常活动能力降低引起的老年疾病的影响［J］.中国老年学杂志,2019,39(9):2299-2303.
［6］李剑兰,余璇,胡青婷,等.老年帕金森病与肠道菌群、短链脂肪酸和炎性因子相关性研究［J］.实用老年医学,2021,35(3):282-285.
［7］CRYAN JF,O’RIORDAN KJ,COWAN CSM,et al.The Microbiota-Gut-Brain Axis［J］.Physiol Rev,2019,99(4):1877-2013.
［8］OSADCHIY V,MARTIN CR,MAYER EA.The Gut-Brain Axis and the Microbiome:Mechanisms and Clinical Implications［J］.Clin Gastroenterol Hepatol,2019,17(2):322-332.
［9］NIEMARKT HJ,DE MEIJ TG,VAN GANZEWINKEL CJ,et al.Necrotizing Enterocolitis,Gut Microbiota,and Brain Development:Role of the Brain-Gut Axis［J］.Neonatology,2019,115(4):423-431.
［10］KOWALSKI K,MULAK A.Brain-Gut-Microbiota Axis in Alzheimer’s Disease［J］.J Neurogastroenterol Motil,2019,25(1):48-60.
［11］KAROLY HC,MUELLER RL,BIDWELL LC,et al.Cannabinoids and the Microbiota-Gut-Brain Axis:Emerging Effects of Cannabidiol and Potential Applications to Alcohol Use Disorders［J］.Alcohol Clin Exp Res,2020,44(2):340-353.
［12］TERATANI T,MIKAMI Y,NAKAMOTO N,et al.The liver-brain-gut neural arc maintains the T(reg)cell niche in the gut［J］.Nature,2020,585(7826):591-596.
［13］HOLLISTER EB,GAO CX,VERSALOVIC J.Compositional and functional features of the gastrointestinal microbiome and their effects on human health［J］.Gastroenterology,2014,146(6):1449-1458.
［14］中国后循环缺血专家共识组.中国后循环缺血的专家共识［J］.中华内科杂志,2006,45(9)：786-787.
［15］袁肇凯,王天芳.中医诊断学［M］.北京:中国中医药出版社,2007:158-173.
［16］SCHOELER M,CAESAR R.Dietary lipids,gut microbiota and lipid metabolism［J］.Rev Endocr Metab Disord,2019,20(4):461-472.
［17］ADAK A,KHAN MR.An insight into gut microbiota and its functionalities［J］.Cell Mol Life Sci,2019,76(3):473-493.
［18］STRANDWITZ P.Neurotransmitter modulation by the gut microbiota［J］.Brain Res,2018,1693(Pt B):128-133.
［19］DINAN TG,CRYAN JF.Gut-brain axis in 2016:Brain-gut-Microbiota axis-mood,metabolism and behaviour［J］.Nat Rev Gastroenterol Hepatol,2017,14(2):69-70.
［20］KENNEDY PJ,CRYAN JF,DINAN TG,et al.Kynurenine pathway metabolism and the Microbiota-gut-brain axis［J］.Neuropharmacology,2017,112(Pt B):399-412.
［21］QUIGLEY EMM.Microbiota-Brain-Gut Axis and Neurodegenerative Diseases［J］.Curr Neurol Neurosci Rep,2017,17(12):94.
［22］金晶,刘雨朦,伦志刚,等.缺血性脑卒中与脑-肠轴关系的研究进展［J］.中华神经医学杂志,2020(5):514-518.
［23］林菲菲,何春风,林德.儿童功能性便秘患者肠道菌群及血清脑肠肽水平的变化［J］.中国微生态学杂志,2020,32(6):692-694，699.
［24］黄健庭,杜文兵,徐珊.眩晕病的中医证候要素及证型分布规律研究［J］.广州中医药大学学报,2020,37(7):1398-1402.
［25］任平,夏天,李平,等.脾虚腹泻患者肠道菌群的研究［J］.中医杂志,1992,33(6):33-34.
［26］卢林,刘伦翠,卢晋,等.中药复方对脾虚湿盛泄泻患者肠道及舌部微生态影响的研究［J］.牡丹江医学院学报,2009,30(5):70-73.
［27］关婷,黄海阳,黄纯美,等.不同药性归脾经中药对肝郁脾虚证动物模型肠道微环境的影响［J］.中药新药与临床药理,2021,32(4):511-517.
［28］司原成.健脾益气针法对肥胖鼠肠道菌群及肠黏膜TLRs/TRAF6信号通路的调控研究［D］.成都:成都中医药大学,2017.
［29］王冬.谷越涛从肝脾论治眩晕临床经验［J］.国际中医中药杂志,2020，43(3):279-281.
［30］杨平,宋囡,贾连群,等.从“汁沫与血相抟”论线粒体功能障碍与动脉粥样硬化［J］.中华中医药学刊,2020,38(10):55-58.
［31］屈小虎,黄玲,陈慧,等.脾气虚证和脾不统血证模型大鼠脾主肌肉实质的探讨［J］.中国中西医结合杂志,2019,39(2):217-222.
［32］王妮,张娜,李铁,等.人参干预脾气虚体质的非靶向代谢组学研究［J］.中国中药杂志,2020,45(2):398-404.
［33］郑琴,罗俊,章德林,等.基于糖代谢、脂代谢和能量代谢探讨脾脏与阿尔茨海默病的关系［J］.中国实验方剂学杂志,2020,26(5):178-185.
Memo
基金项目：广东省自然科学基金项目（2018A030310060）