[1] ZHU QingLI Ming WANG JiayinWUTaidingCHEN Zonghai ZHU YaoCHEN Longju,.Preliminary Study on the Mechanism of Curcumin Regulating Iron Death in Osteosarcoma Cells through SLC7A11 [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2024, (02): 17-21. [doi: 10.13424/j.cnki.jsctcm.2024.02.004]
Preliminary Study on the Mechanism of Curcumin Regulating Iron Death in Osteosarcoma Cells through SLC7A11
- Article number:
- 2096-1340(2024)02-0017-05
- ZTFLH:
- B21,R221
- Code:
- A
- Abstract:
- Abstract： Objective To investigate the effect of curcumin (Cur) on ferroptosis in osteosarcoma U-2 OS cells based on SLC7A11.Methods The CCK-8 method was used to detect the effect of Cur at 0,5,10,20,40,and 80 μmol·L-1 on the inhibition of U2-OS cells,and the morphological changes of cells were observed under an inverted microscope.Secondly,U-2 OS cells cultured in vitro were randomly divided into Con group (0 μmol·L-1),Fer-1 group (4 μmol·L-1),Cur group (40 μmol·L-1),and Cur+Fer-1 group (40 μmol·L-1+4 μmol·L-1),and the expression of SLC7A11 was detected using Western blot.Results Cur significantly inhibited U-2 OS cells at concentrations of 10-80 μmol·L-1.Further research has shown that Cur can significantly induce ferroptosis in U-2 OS cells and downregulate the expression of SLC7A11.Conclusion Cur can induce ferroptosis in U-2 OS cells,and its mechanism may be achieved by downregulating SLC7A11 to achieve ferroptosis.
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Memo
基金项目：国家自然科学基金项目（81260192）