[1] Sun Lijun, Jiang Mei, Sun Yuru, et al. Experimental Study on Learning and Memory Ability and Expression of Caveolin － 1 Protein in Hippocampus of Ｒats with Kidney Deficiency [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2018, (06): 91-96. [doi: 10.13424/j.cnki.jsctcm.2018.06.030]
Experimental Study on Learning and Memory Ability and Expression of Caveolin － 1 Protein in Hippocampus of Ｒats with Kidney Deficiency
- Article number:
- 2096－1340(2018)06－0091－06
- Keywords:
- “kidney storing will” ; constitution of kidney deficiency; learning and memory; hippocampus; Caveolin －1
- ZTFLH:
- Ｒ2 －031
- Code:
- A
- Abstract:
- Objective To study the learning and memory abilities and the expression of Caveolin －1 protein in hippocampus of rats with kidney deficiency，and to explore the material basis and neurobiological mechanism of“kidney storing will”from the perspective of synaptic plasticity． Method The rat model of congenital insufficiency and acquired intimidation of kidney deficiency was established by "cat scare rat" model． The rats were divided into four groups: model group， kidney － invigorating group，heart － tonifying group and liver － soothing group． The kidney － invigorating group，heart － tonifying group and liver － soothing group were given Liuwei Dihuang Pills，Tianwang Buxin Bolus and Xiaoyao pills re spectively． The blank group was produced from normal pregnant rats． Morris water maze test was used to test the learning and memory abilities of rats in each group，immunohistochemical method was used to detect the positive expression ofCaveolin －1 protein in hippocampus． Ｒesult Compared with the blank group， the escape latency，the total distance and the time of first crossing the target area in the model group increased significantly，while the stay time in the target area and the number of Caveolin －1 immunoreactive positive cells in the hippocampus decreased significantly． Compared with the model group， the escape latency， the total distance and the time of first crossing the target area in the kidney － invigorating group and heart － tonifying group increased significantly，while the stay time in the target area and the number of Caveolin －1 immunoreactive positive cells in the hippocampus increased significantly． Compared with the heart － tonifying and liver － soothing groups， the escape latency and the time of first crossing the target area were shortened， the total distance was significantly reduced， the stay time in the target area and the expression of Caveolin － 1 in the hippocampus were significantly increased． Conclusion The learning and memory ability of rats with kidney deficiency was degraded and the intrinsic mechanism might be related to the decrease of Caveolin －1 protein expression in the hippocampus of rats with kidney － deficiency syndrome; Liuwei Dihuang Pills can improve the learning and memory ability of rats with kidney － deficiency syndrome by up － regulating the expression of Caveolin －1 protein; the improvement effect of heart － tonifying and liver － soothing drugs is inferior to kidney － invigorating drugs，highlighting the kidney and learning and memory． The re lationship between them is consistent with the theory of“kidney storing will”
References:
[1]孙理军，孙耀光，张琪，等．肾虚质大鼠生长发育和学习 记忆能力与肾藏志理论相关性研究［Ｊ]．中华中医药杂 志，２０１６，３１（１）：３６－３８．
[2]薛昶，孙理军，郝蕊．肾虚体质大鼠生理指标变化的实 陕 西 中 医 药 大 学 学 报 ２０１８年 １１月第 ４１卷第 ６期 ·９６· ＪｏｕｒｎａｌｏｆＳｈａａｎｘｉＵｎｉｖｅｒｓｉｔｙｏｆＣｈｉｎｅｓｅＭｅｄｉｃｉｎｅ Ｎｏｖ．２０１８ Ｖｏｌ．４１ Ｎｏ．６ 验研究［Ｊ]．陕西中医，２００８，２９（４）：５０５－５０６
[3]孙瑜鉓，孙理军，孙耀光，等．补肾中药对肾虚质大鼠 生长发育及脑组织胆碱乙酰化酶含量的影响［Ｊ]．中 国中医药信息杂志，２０１７，２４（９）：４８－５０．
[4]孙理军，孙耀光，孙瑜鉓，等．基于 ＳＮＡＰ－２５表达的肾 虚质大鼠突触可塑性实验研究［Ｊ]．中国中医基础医 学杂志，２０１７，２３（９）：１２１７－１２１９，１２７９．
[5]孙理军，孙耀光，孙瑜鉓，等．肾虚质大鼠生长发育与 蛋白激酶 Ｃ表达的研究［Ｊ]．中华中医药杂志，２０１８，３３ （３）：１１３４－１１３６．
[6]林蕾，张良成，郭永正．突触可塑性及相关物质的研究 进展［Ｊ]．中华全科医学，２００９，（９）：９９５－９９６．
[7]孙理军，李翠娟，王震，等．关于肾虚质实验动物模型相 关问题的研究与思考 ［Ｊ]．时珍国医国药，２０１２，２３ （１２）：３０９４－３０９５．
[8]陈小野，周永生，樊雅莉，等．１０种大鼠的中医体质学初 步研究［Ｊ]．实验动物科学与管理，２０００，１７（４）：３３ －３９．
[9]孙瑜鉓，孙耀光，张琪，等．肾虚质大鼠学习记忆能力 与海马 ＮＭＤＡ受体表达的实验研究［Ｊ]．中国中西医结 合杂志，２０１６，３６（５）：５９７－６０１．
[1０]段婷婷．情绪记忆的海马突触可塑性机制研究［Ｄ]． 合肥：中国科学技术大学，２０１３．
[1１]邹伟，王红霞，刘晶，等．大鼠脑内 ｃａｖｃｏｌｉｎ－１蛋白的 表达及其在分辨学习中的作用［Ｊ]．生理学报，２００６，５８ （５）：４２９－４３４．
Memo
基金项目:陕西省重点实验室研究项目( 17JS033) ( 16JS026); 陕西省自然基础项目( 2015JM8457) 收稿日期:2018 －04 －08 编辑:文颖娟