[1] Cao Limin, Guan Shanshan, Zhang Peng,.Analgesic Effect of Pueraria Tongluo Granules [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2018, (01): 79-82,87. [doi: 10．13424/j．cnki．jsctcm．2018．01．026]
Analgesic Effect of Pueraria Tongluo Granules
- Article number:
- 2096－1340(2018)01－0079－05
- ZTFLH:
- Ｒ285. 5
- Code:
- A
- Abstract:
- To observe the analgesic effect and the mechanism of pueraria Tongluo granules． Method: 48 eligible mice were randomly divided into: model group，puerarin Tongluo low，medium and high dose groups ( 3． 0，6． 0， 12． 0g · kg－ 1) ，in terms of crude drug，hereinafter referred to as GG low group，GG Group，GG high group) ． Themodel group was treated with 0． 5% CMC － Na suspension ( 0． 2mL /10g) ，and hot plate method was used to observe the change of pain threshold of mice． 50 eligible mice were randomly divided into model group，GG low group，GG Group， GG high group and aspirin group( 0． 2g / kg) ． 0． 5% CMC － Na suspension ( 0． 2mL / 10g) was given to the model group． The mice in each group were injected intraperitoneally with 0． 6% acetic acid ( HAC) at 0． 1mL / 10g one hour after the administration． Mice writhing reaction times were recorded and the inhibition rate was calculated． 50 eligible mice were randomly divided into model group，GG low group，GG group，GG high group and blank control group，except the blank control group，the other four groups were subcutaneously injected reserpine 1． 0mg / kg for continuous 10 days，the effect of pueraria granules on 5 － HT content in mouse brain was observed． Ｒesult: Pain threshold: the pain thresholds of the four groups of mice before treatment and 0． 5h after administration were not statistically significant ( P≥0． 05) ． Compared with the model group，the pain threshold of the GG high group was significantly increased at 1h，1． 5h and 2h ( P ＜ 0． 01， P ＜ 0． 01，P ＜ 0． 05，respectively) ． Compared with the model group，the pain threshold of the GG group was significant- ly increased at 1． 5h and 2h after administration ( P ＜ 0． 05) ，the difference was statistically significant ( P ＜ 0． 05) ． Compared with the model group at each time point，the difference of pain threshold of the GG low group was not statisti- cally significant ( P≥0． 05) ． Writhing times: Compared with the model group，the GG low，medium and high dose group and aspirin group could significantly reduce the writhing times of mice induced by glacial acetic acid，and the difference was statistically significant ( P ＜ 0． 05) ． 5 － HT content in brain: The neurotransmitter 5 － HT in the mice of the model group was significantly lower than that in the normal mice ( P ＜ 0． 05) ，GG high group showed a significant increase in 5 － DT and the difference had statistical significance( P ＜ 0． 05) compared with the model group，5 － DT contents in GG and GG high groups were also increased to some extent while the difference had no statistical significance ( P≥0． 05) ．
References:
[1]金玉青，洪远林，李建蕊，等． 川芎的化学成分及药理作
用研究进展［J]． 中药与临床，2013，4( 3) : 44 － 48．
[2]张素慧，宁炼，石劲敏，等． 葛根总黄酮经鼻给药对硝酸
甘油致偏头痛模型大鼠的保护作用［J]． 中国新药与临
床杂志，2013，32( 5) : 394 － 398．
[3]张军田，杜冠华． 现代药理实验方法［M]． 北京: 中国协
和医科大学出版社，2013: 1381 ～ 1382．
[4]郭倩瑶，郭建生，刘顶鼎，等． 芎麻汤不同提取物对硝酸
甘油偏头痛模型大鼠血浆 NO、CGＲP、5 － HT、ET 含量
的影响［J]． 西安交通大学学报医学版，2013，34( 3) :
393 － 396．
[5]谭亮． 偏头痛发病机制的研究进展［J]． 中国临床神经
外科杂志，2012，17( 9) : 571 － 573．
[6]刘顶鼎，郭建生，曾贵荣，等． 芎麻汤及其提取物对利血
平低 5 － HT 型偏头痛模型小鼠的影响［J]． 湖南中医
药大学学报，2012，32( 3) : 17 － 19．
Memo
收稿日期: 2017 － 09 － 20 编辑:文颖娟
基金项目:陕西省中医管理局中医药科研课题( 15 － LC077)
作者简介:曹利民( 1962 － ) ，男，本科，主任医师，主要从事神经系统疾病的防治工作。E － mail: 346619410@ qq． com
通讯作者:张鹏( 1983 － ) ，男，硕士研究生，榆林市中医医院康复科主任，主治医师，研究方向: 针药结合对神经系统
疾病防治及康复。E － mail: 346619410@ qq． com