[1] LIU Yan ZOU Yuanrong HUANG Jianping WANG Hongyan XIA Zebing WANG Qilong JING Yajiang PENG Liang GAO Jing ZHANG Gang WANG Danyang YAN Yonggang,.Exploration of the Mechanism of Anti Arrhythmia Effect of Roasted Licorice by UPLC-TOF-MS,GC-MS Combined with Network Pharmacology [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2023, (04): 84-93. [doi: 2096-1340(2023)04-0084-10]
Exploration of the Mechanism of Anti Arrhythmia Effect of Roasted Licorice by UPLC-TOF-MS,GC-MS Combined with Network Pharmacology
- Article number:
- 10.13424/j.cnki.jsctcm.2023.04.016
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract： Objective To combine the chemical components of roasted licorice with network pharmacology and explore the mechanism of its anti arrhythmia effect.Methods UPLC-TOF-MS and GC-MS techniques were used to analyze the chemical components of roasted licorice.TCMSP database was used to screen active ingredients,Swiss Target Prediction database and Gene Cards database were used to collect drug action targets and disease targets,Cytoscape 3.8.2 software was used to screen core targets,and protein interaction network analysis,GO and KEGG enrichment analysis were carried out on core targets.Cytoscape 3.8.2 was used to construct drug key component core target pathway diagram,and Macromolecular docking verification was completed by Autodocking vina 1.2.0 software.Results A total of 289 chemical components were analyzed,among which 16 components such as isoliquiritigenin and tyrosine were key components for anti arrhythmias;The protein interaction network screened 55 core targets,including ALB,AKT1,VEGFA,EGFR,etc.GO enrichment resulted in a total of 476 entries,and KEGG resulted in a total of 130 pathways.The results of Macromolecular docking showed that isoliquiritigenin,daidzein and citrinin had good docking activity with the core target.Conclusion Roasted licorice may play an antiarrhythmic role by regulating multiple targets such as ALB,AKT1,VEGFA,and EGFR,as well as participating in cancer pathways,Proteoglycan in cancer,endocrine resistance,and resistance to EGFR Tyrosine kinase inhibitor.
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Memo
基金项目：国家自然科学基金项目（81973592）； 陕西省中医药管理局项目（2021-GJ-JC004）