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[1] LI Min LIU Xiao XU Xiaobo LI Tianran,.Anti Inflammatory Effect of Gancao Xiexin Decoction Combined with Dragons Blood on DSS Induced UC Model Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (01): 77-83. [doi: 10.13424/j.cnki.jsctcm.2022.01.017]

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Anti Inflammatory Effect of Gancao Xiexin Decoction Combined with Dragons Blood on DSS Induced UC Model Rats

Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R] Volumes & Issues: 2022 01 pagination: 77-83 Column: Publication date: 2022-02-01

Article number:: 2096-1340(2022)01-0077-06

Author(s):: LI Min LIU Xiao XU Xiaobo LI Tianran; Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China

Keywords:: Key words： Gancao Xiexin decoction; Dragons blood; Ulcerative colitis; Colonic pathology; Inflammatory cytokines

ZTFLH:: R289

DOI:: 10.13424/j.cnki.jsctcm.2022.01.017

Code:: A

Abstract:: Abstract： Objective To investigate the antiinflammatory effect of Gancao Xiexin decoction Combined with Dragon's blood on ulcerative colitis (UC) induced by dextran sulfate sodium (DSS).Methods 100 SPF SD rats were randomly divided into 25 blank groups and 75 model groups.The model rats were made by 3% DSS free drinking method.After successful modeling,the rats were divided into model group,sulfasalazine (SASP) group and Gancao Xiexin decoction + Dragon's blood group,with 25 rats in each group.The Gancao Xiexin decoction + Dragon's blood group was given Gancao Xiexin decoction 6 g?(kg?d)-1 Dragon's blood　375 mg ? (kg ? d)-1 suspension was administered by gavage,sulfasalazine group 450 mg ? (kg ? d)-1 suspension was administered by gavage every day,blank group and model group were administered by gavage of equal volume normal saline 25 mL?case-1 every day for 7 days.The general condition of rats and the degree of inflammation after colonic HE staining were observed.The rats were scored by disease activity index (DAI),colonic mucosal injury (CMDI) and histopathology (HS).The serum content ofinterleukin6 (IL-6) and tumor necrosis factor α( TNF-α) were detected by ELISA .Results The body mass of the model group was lower than that of the blank group,DAI,CMDI,HS scores and serum IL6 and TNFα.The body mass of rats in Gancao Xiexin decoction + Dragon's blood group and sulfasalazine group was higher than that in model group,DAI,CMDI,HS scores,serum IL6 and TNFα The content of HS score and serum IL6 and TNFα in Gancao Xiexin decoction + Dragon's blood group were significantly lower than those in model group (P < 0.05).The content was lower than that of sulfasalazine group.Conclusion Gavage of Gancao Xiexin decoction Combined with Dragon's blood can improve the symptoms of DSS induced UC model rats,reduce the colon DAI,CMDI and HS scores of UC rats,and inhibit the serum IL6 and TNFα of UC rats.It has a clear anti ulcerative colitis effect.

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基金项目：南充市市校战略合作科技项目（18SXHZ0571）

Last Update: 2022-02-10