[1] CHI Ningjuan LIU Mingyi XU Fangqin SHI Xiaopeng YANG Zhifu ZHAO Chao ZHAO Tao WANG Jingwen,.Effect of Danggui Zhuyu Capsule on NF-κB Mediated Inflammatory Response in Cerebral Cortex Tissue of CI/RI Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2024, (03): 99-104. [doi: 10.13424/j.cnki.jsctcm.2024.03.014]
Effect of Danggui Zhuyu Capsule on NF-κB Mediated Inflammatory Response in Cerebral Cortex Tissue of CI/RI Rats
- Article number:
- 2096-1340(2024)03-0099-06
- Keywords:
- Key words：Danggui Zhuyu capsules; Cerebral ischemia-reperfusion injury; Inflammatory response; Nuclear Factor κB
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract：Objective To explore the effects of Danggui Zhuyu capsules on the nuclear factor κB (NF-κB) signaling pathway and inflammatory response in the cortical tissue of the injured side of the brain at different time points of cerebral ischemia-reperfusion injury (CI/RI) in rats.Methods 120 rats were randomly divided into a sham surgery group,a model group,and an Angelica sinensis stasis removing group.Each group was further divided into four subgroups based on reperfusion for 24 hours,48 hours,72 hours,and 10 days,respectively.Except for the sham surgery group,the other groups prepared rat MCAO models using thread occlusion method.After 2 hours of ischemia,the rats were given gastric lavage after waking up.The sham surgery group and model group were given distilled water of 10 mL·kg-1 by gastric lavage,while the Danggui Zhuyu group was given gastric lavage 2.00 g·kg-1,once a day.Brain tissue was taken for TTC staining to determine infarct area,HE staining to observe histopathological changes,and RT-PCR to determine TNF-α、IL-8、ICAM-1and MCP-1 mRNA expression,Western blotting for IKKβ and NF-κB65 protein expression.Results The results showed that Danggui Zhuyu capsules could significantly reduce the area of cerebral infarction at various time points of reperfusion (P<0.05);Effectively improving pathological damage to cerebral cortex tissue;Significantly reduce TNF after reperfusion for 24 hours to 10 days- α MRNA,ICAM-1 mRNA,MCP-1 mRNA,and p-NF-B65 protein expression were reduced,while IL-8 mRNA and IKK protein expression were reduced from 24 hours to 48 hours after reperfusion (P<0 05).Conclusion Danggui Zhuyu Capsule has a neuroprotective effect,and its mechanism of action may be through regulating IKKβ and p-NF-κB65 proteins,and reducing TNF-α mRNA、ICAM-1mRNA、MCP-1 mRNA and p-NF-κB65 mRNA expression,exert neuroprotective effects by inhibiting inflammatory responses.
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Memo
基金项目：国家自然科学基金面上项目（81573549）