[1] HUI JianpingZHANG YaozhiLIU HaitaoWANG JunLI LongmeiSHEN Shuwen,.Study on the Regulation of HSP27 and MAD2 Expression in Gastric???? Precancerous Lesions in Rats by Jinguo Weikang Capsule [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2023, (06): 60-66. [doi: 10.13424/j.cnki.jsctcm.2023.06.010]
Study on the Regulation of HSP27 and MAD2 Expression in Gastric???? Precancerous Lesions in Rats by Jinguo Weikang Capsule
- Article number:
- 2096-1340(2023)06-0060-07
- Keywords:
- Key words： Jinguo Weikang capsules; Gastric precancerous lesions; Chronic atrophic gastritis; HSP27; MAD2
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- Abstract： Objective To observe the effect of Jinguo Weikang Capsule on the expression of HSP27 and MAD2 proteins in the gastric mucosa of rats with gastric precancerous lesions.Methods 85 rats were randomly divided into a blank group of 10 and a model group of 75.The blank group was given a normal diet,while the modeling group used a composite modeling method consisting of Nmethyl-N-nitro-Nnitrosoguanidine (MNNG),ethanol,ammonia,and hunger and satiety disorders.Randomly divide the rats after modeling into model group,high,medium,and low dose groups of Jinguo Weikang capsules,and vitamin enzyme group.Each medication group was given corresponding drugs by gavage,while the blank group and model group were given equal amounts of physiological saline by gavage once per day for 12 consecutive weeks.After 12 weeks,all rats were euthanized,and gastric tissue was taken.Pathological changes in the gastric mucosa tissue of each group of rats were observed under light microscopy;Immunohistochemical method was used to detect the expression of HSP27 and MAD2 proteins in gastric mucosal tissue.Results The expression levels of HSP27 and MAD2 proteins in the model group were significantly higher than those in the blank group (all P<0.05).The expression levels of HSP27 and MAD2 proteins in each treatment group were significantly lower than those in the model group (all P<0.05),and the highdose group of Jinguo Weikang Capsule was significantly lower than the Weimycin group (all P<0.05).Conclusion Jinguo Weikang Capsule can reverse the occurrence of gastric precancerous lesions,and the mechanism of action may be related to its ability to inhibit the expression of HSP27 protein and control the expression of MAD2 protein.
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Memo
基金项目：陕西省自然科学基金项目（2021JM482）；陕西省中医药管理局项目（2019-ZZ-JC004）；国家级沈舒文名中医工作室建设项目