Pre Next

PDF Download

[1] WANG Hui SHAO Qing ZHANG Yan WANG Wenjie YANG Miaomiao HU Jiewen ZHANG Ling,.Preparation and in Vitro Transdermal Properties of Sophora Alopecuroides Total Alkaloid Diol Liposome [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2023, (03): 71-75. [doi: 10.13424/j.cnki.jsctcm.2023.03.014]

Copy

Preparation and in Vitro Transdermal Properties of Sophora Alopecuroides Total Alkaloid Diol Liposome

Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R] Volumes & Issues: 2023 03 pagination: 71-75 Column: Publication date: 2023-05-31

Article number:: 2096-1340(2023)03-0071-05

Author(s):: WANG Hui¹ SHAO Qing¹ ZHANG Yan¹ WANG Wenjie¹ YANG Miaomiao¹ HU Jiewen¹ ZHANG Ling²; 1.Xi’an Mental Health Center,Xi’an 710061,China;
2.Xi’an Jiaotong University Affiliated Red Society Hospital,Xi’an 710000,China

Keywords:: Key words： Total alkaloids of Sophora alopecuroides (SATA); Diol liposomes; Ethanol; Propylene glycol; Transdermal delivery carrier; Confocal laser scanning electron microscopy

ZTFLH:: R284

DOI:: 10.13424/j.cnki.jsctcm.2023.03.014

Code:: A

Abstract:: Abstract： Objective In the process of preparing Sophora alopecuroides total alkaloids liposomes,two watersoluble permeation enhancers,ethanol and propylene glycol,were added to obtain a new and stable transdermal delivery system,enhancing the skin transmission of Sophora alopecuroides total alkaloids (SATA).Methods Injecting method was used to prepare diol liposomes,and their morphology,size,and Zeta potential were characterized.The encapsulation efficiency,vesicle stability,and skin permeation characteristics were important factors for optimizing the ratio of ethanol and propylene glycol.In vitro skin permeation experiments were conducted on mouse skin using Franz diffusion cell to compare the cumulative permeation amount of mouse skin.The penetration depth and fluorescence intensity of sophora alopecuroides total alkaloid diols liposomes containing fluorescein rhodamine B in isolated mouse skin were observed by laser confocal scanning electron microscopy.Results The results showed that when ethanol:propylene glycol=7∶3 (w/w),the stability of diol liposomes was the best,the encapsulation efficiency was the highest (89.13±0.42)%,the particle size was the smallest (103.00±16.15) nm,and the transdermal depth was (170 μm).Maximum and strongest fluorescence intensity (MaxFI=160 AU).Conclusion The prepared bitter bean seed total alkaloid diol liposome (ethanol:propylene glycol=7∶3,w/w) is an efficient and stable transdermal delivery carrier.

References:

［1］刘伟,张扬星,杨新洲,等.苦豆子总碱降血糖作用及其机制研究［J］.天然产物研究与开发,2021,12(10):1991-1997.
［2］施晓莹,谈文盼,宋丽军,等.HPLC-MS检识苦豆子总碱双层包衣微丸入血成分［J］.辽宁中医药大学学报,2019,21(4):65-69.
［3］王慧,张晓红,张燕,等.乙醇脂质体、二元醇脂质体及传递体对多奈哌齐经皮转运的对比性研究［J］.中国医院药学杂志,2018,38(3):219-222.
［4］邢文善,路芳,徐阳.经皮给药新型脂质体——二元醇质体的研究进展［J］.中国医药导刊,2018,20(4):221-224.
［5］宋娟,于绪东,张振宇,等.茶多酚二元醇脂质体的制备及质量评价［J］.中国麻风皮肤病杂志,2018,34(7):407-410.
［6］陈积,赵小倩,马彦巧,等.复方莪术油脂质体凝胶的制备及体外透皮作用［J］.中国新药杂志,2018,27(7):830-838.
［7］靖会,赵惠茹,张宇洁,等.RPHPLC法测定雷公藤甲素脂质体中雷公藤甲素的含量［J］.陕西中医学院学报,2006,29(1):57-58.
［8］张建平,魏玉辉,张建强,等.苦豆子总碱温度敏感性凝胶的质量控制和稳定性研究［J］.中国医院药学杂志,2016,36(4):632-635.
［9］Touitou E,Dayan N,Bergelson L,et al.Ethosomes - novel vesicular carriers for enhanced delivery:characterization and skin penetration properties［J］.J Control Release,2000,65(2):403-418.
［10］Zhang Y,Ng W,Hu J,et al.Formulation and in vitro stability evaluation of ethosomal carbomer hydrogel for transdermal vaccine delivery［J］.Colloids Surf B Biointerfaces，2018,163(21):184-191.
［11］杨丽.醇质体促进脂溶性药物经皮渗透的机制研究［D］.广州：南方医科大学,2017.
［12］王慧,邵青,张燕,等.尼古丁二元醇脂质体温敏凝胶的制备及体外评价［J］.西北药学杂志,2022,37(6):100-104.
［13］袁海玲,胡继民,魏玉辉,等.布洛芬二元醇脂质体的制备与评价［J］.中国医院药学杂志,2012,32(14):1111-1114.
［14］徐东伟,刘同祥.乙醇注入法制备柚皮素脂质体及其质量评价［J］.中南药学,2015,12(13):1266-1269.
［15］王慧,张玲,邵青,等.二元醇脂质体对多奈哌齐经皮转运的协同促进作用［J］.中国新药杂志,2022,31(20):2051-2055.
［16］Zhang JP,Wei YH,Zhou Y,et al.Ethosomes,binary ethosomes and transfersomes of terbinafine hydrochloride:a comparative study［J］.Archives of pharmacal research,2012,35(12):109-117.
［17］邢宇,李雄,于颖.银杏内酯B脂质体的处方与制备工艺研究［J］.中国现代应用药学,2021,38(6):697-703.
［18］翁金燕,刘瑶,丁佳圣,等.3种不同溶媒介质脂质体的制备及其初步稳定性考察［J］.中国药师,2018,21(5):824-827.
［19］袁海玲,杨煊,张双金,等.布洛芬二元醇脂质体温敏凝胶的处方筛选与体外释放研究［J］.中国药房,2015,26（10）:1389-1392.
［20］黄永佳,刘娟,赵静,等.负载氢醌的甘油丙二醇二元醇质体的制备及体外经皮渗透研究［J］.重庆医科大学学报,2020,45(6):824-827.
［21］杨丽华,马春,李淑玲,等.和厚朴酚注射用冻干脂质体治疗血管性痴呆大鼠的神经保护作用机制［J］.陕西中医药大学学报,2017,40(4):114-119.
［22］Smith MC,Crist RM,Clogston JD,et al.Zeta potential:a case study of cationic,anionic,and neutral liposomes.Anal Bioanal Chem［J］.2017,409(24):5779-5787.
［23］殳叶婷,许妍,高洁,等.磷脂组成对荧光修饰环糊精脂质体皮肤递送效果的影响［J］.中国药学杂志,2020,55(13):1094-1097.
［24］牛小倩.盐酸多奈哌齐醇质体的制备、表征及其经皮机制研究［D］.杭州:浙江大学,2019.
［25］Joakim K，Eneko L.In vivo studies investigating biodistribution of nanoparticle encapsulated rhodamine B delivered via dissolving microneedles［J］.J Controlled Release,2017,265(10)：57.

Memo

基金项目：国家自然科学基金项目（81202938）；西安市科学技术局重点实验室(201805051ZD2CG35)

Last Update: 2023-05-19