[1] SONG Jian YUAN Minhui CUI Binghui CHU Yupeng MENG KaiqiangHUANG Yujuan HUI Jianping LIU Zhenghui WANG Jiehong SHEN Shuwen,.Intervention of Jinguo Weikang Capsule on Wnt/Lgr5 Signal Pathway in PLGC Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (05): 102-108. [doi: 10.13424/j.cnki.jsctcm.2022.05.020]
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Intervention of Jinguo Weikang Capsule on Wnt/Lgr5 Signal Pathway in PLGC Rats

Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R] Volumes & Issues: 2022 05 pagination: 102-108 Column: Publication date: 2022-09-20
Article number:
2096-1340(2022)05-0102-07
Author(s):
SONG Jian1 YUAN Minhui2 CUI Binghui1 CHU Yupeng1 MENG Kaiqiang1HUANG Yujuan1 HUI Jianping1 LIU Zhenghui2 WANG Jiehong2 SHEN Shuwen1
1.Shaanxi University of ChineseMedicine,Shaanxi Xianyang 712046,China;
2.Affiliated Hospital of Shaanxi University of Chinese medicine,Shaanxi Xianyang 712000，China
Keywords:
Key words： Jinguo Weikang capsule; Precancerous lesions of gastric cancer; Rats; Wnt/Lgr5 signal pathway; Animal experiments; Mechanism research
ZTFLH:
R256.3
DOI:
10.13424/j.cnki.jsctcm.2022.05.020
Code:
A
Abstract:
Abstract： Objective To explore the effect of Jinguo Weikang Capsule on intervention mechanism of Wnt1,β-catenin,Lgr5 genes and their protein expression.Methods 90 male SD rats were randomly divided into blank group (12 rats) and model group (78 rats).The PLGC model was reproduced by MNNG combined with concentrated saline gavage and abnormal hunger and satiety.After the model was established successfully,they were randomly divided into model group,high,medium and low dose treatment groups of traditional Chinese medicine and vitamin treatment group.They were given normal saline,different doses of Jinguo Weikang capsule and vitamin for 6 weeks.HE staining and AB-PAS staining were used to observe the pathological changes of rat gastric mucosa under light microscope;Detection of Wnt1,β-catenin,Lgr5 gene and protein expression in Wnt/Lgr5 signal pathway by RT-PCR.Results Compared with the model group,the pathological changes of gastric mucosal epithelium in the Jinguo Weikang Capsule treatment group were improved,and the expression of Wnt1 mRNA，β-catenin mRNA，Lgr5 mRNA decreased significantly (P<0.01).Conclusion Jinguo Weikang capsule can reduce the protein expression and gene transcription of Wnt1，β-catenin and Lgr5，promote cell apoptosis and reduce the possibility of gastric cancer,so as to achieve the purpose of treating PLGC.

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Memo

基金项目：陕西省教育厅专项科学研究计划项目（18JK0225）；陕西中医药大学科研计划项目（2017PY0国家级大学生创新创业训练计划项目（201910716024）

Last Update: 2022-09-23
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