[1] YAN Hao LIU Xiaoxiao YU Chunxue SUN Keqiang CHENG FanWANG Hu SONG Xiao,.Study on Mechanism of Huangqi Gegen Decoction inTreating Diabetes Based on Network Pharmacology and Molecular Docking [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (04): 126-132. [doi: 10.13424/j.cnki.jsctcm.2022.04.028]
Study on Mechanism of Huangqi Gegen Decoction inTreating Diabetes Based on Network Pharmacology and Molecular Docking
- Article number:
- 2096-1340(2022)04-0126-07
- Keywords:
- Key words：Huangqi Gegendecoction; Diabetes; Network pharmacology; Molecular docking; Mechanism of action
- ZTFLH:
- R285
- Code:
- A
- Abstract:
- Abstract： Objective To explore the mechanism of Huangqi Gegen decoction in the treatment of diabetes through network pharmacology and molecular docking. Methods Based on the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) database，the effective components and target genes of Huangqi Gegen decoction were screened. The disease related target genes were found through GeneCards and OMIM，and the drug effective component targets were mapped with disease targets；Cytoscape was used to construct drug component target disease network. Using STRING database，the core genes of protein-protein interaction were analyzed. Connect the active ingredient with the first three of the core genes. Bioconductor was used for GO function enrichment and KEGG pathway enrichment analysis. Results 59 active components of Huangqi Gegen decoction and 135 common targets of drugs and diseases were obtained；The core genes in the protein’protein interaction network are EGFR，IL6 and MYC，which involve prostate cancer，p53 signaling pathway，Fluid shear stress and atherosclerosis，AGERAGE signaling pathway indiabetic composites and other pathways. Conclusion It is preliminarily revealed that Huangqi Gegen decoction plays a role in the treatment of diabetes in a complex form of multicomponent，multitarget and multi’channel，which provides a theoretical basis for the clinical application of Huangqi Gegen decoction.
References:
［1］吴耿旭,邓小敏,陈菲,等.中医药治疗2型糖尿病研究进展［J］.世界最新医学信息文摘,2019,19(92):51-52.
［2］赵磊,慕杨娜,梁茂新.消渴(糖尿病)古今用药对比研究［J］.北京中医药大学学报,2016,39(9):769-773.
［3］王凌,刘海英,刘芳洁.黄芪葛根汤治疗老年糖尿病疗效观察及对胰岛素抵抗的影响［J］.新中医,2019,51(9):118-121.
［4］刘倩,范颖,李新,等.黄芪葛根配伍调节糖尿病大鼠血糖血脂炎症的作用与机制［J］.中国实验方剂学杂志,2018,24(11):81-86.
［5］Li S,Zhang B.Traditional Chinese medicine network pharmacology:theory,methodology and application［J］.Chinese Journal of Natural Medicines,2013,11(2):110-120.
［6］Hopkins AL.Network pharmacology:the next paradigm in drug discovery［J］.Nature Chemical Biology,2008,4(11):682-690.
［7］许先进,王存新.分子对接方法在药物发现之外领域的应用［J］.北京工业大学学报,2017,43(12):1872-1880.
［8］Ru JL,Li P,Wang JN,et al.TCMSP:a database of systems pharmacology for drug discovery from herbal medicines［J］.Journal of Cheminformatics,2014,6:13.
［9］张莉华,崔明超,陈少军.基于网络药理学的葛根抗肿瘤潜在机制探讨［J］.天然产物研究与开发,2018,30(4):547-553,615.
［10］宋建波,廖晖,李元平.基于网络药理学探讨黄芪治疗糖尿病肾病作用机制［J］.中草药,2020,51(11):2988-2996.
［11］Trott O,Olson AJ.AutoDock Vina:improving the speed and accuracy of docking with a new scoring function,efficient optimization,and multithreading［J］.Journal of Computational Chemistry,2010,31(2):455-461.
［12］张梅,王宏兰,张坤,等.妊娠期糖尿病小鼠胎盘EGFR的表达与其发病关系的研究［J］.现代生物医学进展,2015,15(17):3232-3235,3289.
［13］安云霞.糖尿病大鼠肺组织cjun/AP1表达的变化及意义［D］.沈阳:中国医科大学,2010.
［14］刘爱东,成敏.原癌基因cmyc在STZ诱导的糖尿病大鼠心脏中的表达［J］.遵义医学院学报,2012,35(3):200-203.
［15］Harada C,Harada T,Mitamura Y,et al.Diverse NFkappaB expression in epiretinal membranes after human diabetic retinopathy and proliferative vitreoretinopathy［J］.Molecular Vision,2004,10:31-36.
［16］聂超.黄芪多糖对P53基因介导的信号传导与胃癌发生发展的相关性研究［D］.南京:南京中医药大学,2014.
［17］Cunningham KS,Gotlieb AI.The role of shear stress in the pathogenesis of atherosclerosis［J］.Laboratory Investigation,2005,85(1):9-23.
［18］王丽,李杰辉.AGEs-RAGE信号通路与糖尿病难愈合创面［J］.中国烧伤创疡杂志,2015,27(6):406-409.
［19］杨超茅,杨志新,马晓玲.AGEs-RAGE信号通路在糖尿病肾病中的作用机制及中医药研究进展［J］.中医学报,2019,34(9):1864-1868.
［20］鲍伟杰,张文健,许世清,等.AGE-RAGE系统在糖尿病神经病变中的作用［J］.生理科学进展,2014,45(2):137-139.
［21］Costal F,Oliveira E,Raposo A,et al.Dual effect of advanced glycation end products in pancreatic islet apoptosis［J］.Diabetes/Metabolism Research and Reviews,2013,29(4):296-307.
Memo
基金项目：国家重点研发计划项目（2019YFC1711200）；陕西省自然科学基础研究计划项目（2019JQ-874）