[1] LIU Zixuan LI Cuijuan KONG Xinyi,.Effect of Pregnant Rats with Kidney Deficiency Constitution on Blood IgG Level and Biochemical Indexes of Offspring Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2022, (02): 62-66. [doi: 10.13424/j.cnki.jsctcm.2022.02.014]
Effect of Pregnant Rats with Kidney Deficiency Constitution on Blood IgG Level and Biochemical Indexes of Offspring Rats
- Article number:
- 2096-1340(2022)02-0062-05
- Keywords:
- Key words： Offspring mice; Normal constitution; Kidney deficiency constitution; Changes of blood biochemical indexes; IgG
- ZTFLH:
- Q95
- Code:
- A
- Abstract:
- Abstract： Objective To compare the changes of blood IgG level and biochemical indexes between normal mice and mice with kidney deficiency constitution,and to explore the effect of parental constitution on the formation of offspring constitution and the occurrence and development of related diseases.Methods According to the principle of “fear hurting kidney” in traditional Chinese medicine,the pregnant mice were stimulated by ultrasonic rat driving instrument,causing them to panic and injure the kidney until the mice stopped threatening after birth,making the mice congenital deficiency.10 mice were randomly selected as the kidney deficiency constitution group,half male and half female,and the general conditions of mice in each group were observed.After modeling,the mice were weighed,the blood was collected from the fundus of the eyes,collected with Eppendorf tube,and the serum was centrifuged for the detection of biochemical indexes and immunoglobulin IgG.Results ① The weight of mice in kidney deficiency constitution group was significantly lighter than that in normal control group.And the body is relatively short,the hair is sparse,timid and easily startled,curled up,with less activity and less appetite,which is consistent with the judgment basis of kidney deficiency constitution.It was statistically significant (P<0.05).② The detection of blood biochemical indexes in mice showed that compared with the normal control group,the blood ALP (alkaline phosphatase),Ca (calcium) and TG (triglyceride) in the kidney deficiency group increased,and the difference was statistically significant (P<0.05 or P<0.01).Although there was no significant difference in creatinine and uric acid between normal control group and kidney deficiency constitution group,the values of blood creatinine and uric acid in kidney deficiency constitution group increased.③ Compared with the normal control group ,the detection of blood IgG concentration in mice showed that,the blood immunoglobulin IgG concentration in the kidney deficiency constitution group was lower than that in the normal control group,and the difference was statistically significant (P<0.05).Conclusion The blood biochemical indexes and the concentration of immunoglobulin IgG in mice with kidney deficiency constitution have changed,indicating that the mother mice with kidney deficiency constitution have an effect on the formation of kidney deficiency constitution and the level of immunoglobulin in offspring mice.
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Memo
基金项目：陕西省中医药管理局中医藏象理论重点研究室建设项目（SZDYJS-2）；陕西省自然科学基础研究计划项目（2021JM-475）；咸阳市中医体质与疾病防治重点实验室重点研发计划项目（2019K01-53）;陕西省中医体质与疾病防治研究重点实验室开放课题