[1] CAI Jia, LI Li, YAN Sheng, et al. Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2021, (02): 61-68. [doi: 10.13424/j.cnki.jsctcm.2021.02.013]
Determination of Five Index Components in Radix Scrophulariae by in Situ Microextraction - Solid Phase Extraction - Liquid Chromatography
- Article number:
- 2096-1340(2021)02-0061-08
- Keywords:
- In situ microextraction; Solid phase extraction liquid chromatography; Radix Scrophulariae; Aucubin; Harpagoside; Angloride C; Harpagoside; Cinnamic acid
- ZTFLH:
- R284
- Code:
- A
- Abstract:
- Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.Objective To establish an in situ microextraction - solid phase extraction - liquid chromatography (SPE -HPLC )method for the rapid extraction, purification and determination of aucubin, angloride C, harpagoside, cinnamic acid and harpagoside in Radix Scrophulariae. Methods The solvent was injected into the micropores of Radix Scrophulari-ae, which was quickly extracted and purified by C18 solid phase extraction. The index components were determined by liquid chromatography. Results When 7. 5g Radix Scrophulariae was used, the solid phase extraction was carried out at 35 ℃ and the micropore size was 1 mm × 1 mm with 1 ml of 50% methanol solution. Conclusion In situ microextraction solid phase extraction liquid chromatography has the advantages of simple in situ pretreatment, few samples, fast sam-pling, less reagent consumption and good purification effect. It realizes the rapid extraction, purification and determina-tion of the index components of Radix Scrophulariae, and provides a new scheme for the rapid determination of the index components of Radix Scrophulariae.
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Memo
基金项目:国自然青年基金项目(81703824);国家中医药管理局标准化建设项目(ZYBZH - Y - HUN -24);湖南省重点研发计划项目(2017NK2261)
通讯作者:许光明,博士,副教授,硕士生导师 E - mail: 1052262329@ qq. Com