[1] Shao Qi, Wang Qianmei, Ma Shanbo, et al. A Research on the Protective Effect of Curcumin on Acute Lung Injury in Mice [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2020, (04): 63-67. [doi: 10.13424/j.cnki.jsctcm.2020.04.014]
A Research on the Protective Effect of Curcumin on Acute Lung Injury in Mice
- Article number:
- 2096-1340(2020)04-0063-05
- ZTFLH:
- R282.5
- Code:
- Ａ
- Abstract:
- Objective To explore the protective effect of curcumin on Acute LungIinjury ( ALI) in mice. Method 40 healthy adult BALB/ c mice were randomly divided into control group, model group, curcumin group and ulinastatin group. The control group was intraperitoneally injected with normal saline, and the other groups were intraperitoneally injected with lipopolysaccharide ( LPS) 20 mg/ kg to establish the mouse model of acute lung injury. The curcumin group was intraperitoneally injected with 200 mg/ kg of curcumin 30 minutes before the modeling of ALI. Ulinastatin group was intraperitoneally injected with ulinastatin ( 1 × 105 U/ kg) 1 hour before the establishment of the ALI model. After 12 hours, mice were sacrificed and blood samples were collected. The serum levels of tumor necrosis factor ( tnf - leutrium) , interleukin -6 ( il -6) , malondialdehyde ( MDA) , and superoxide dismutase ( SOD) were determined by ELISA. Lung tissue was retained to observe the histopathological changes and calculate the lung wet weight/ dry weight ratio ( W/ D) in each group. Result Compared with the control group, serum TNF - beam, il -6 and MDA levels of mice in the model group were significantly increased, and SOD levels were significantly decreased. Compared with the model group, the serum TNF - radiation, il - 6 and MDA levels of mice in the curcumin group and ulinastatin group were significantly reduced, and the level of SOD was significantly increased. Compared with the control group, lung W/ D was significantly increased in the model group. In contrst with the model group, the lung W/ D of mice in curcumin group and ulinastatin group was significantly reduced. The alveolar structure of mice in the model group was seriously damaged, the alveolar space was thickened, and the interstitial exudation was more, while the alveolar structure of mice in the curcumin group and ulinastatin group was relatively complete, and the inflammatory cells exudate less. There was no difference between curcumin group and ulinastatin group. Conclusion Curcumin can reduce the inflammatory response induced by LPS in mice with acute lung injury, and the mechanism may be related to the inhibition of lipid peroxidation.
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Memo
基金项目:国家自然科学基金项目(81601671)
通讯作者:马忠英,主管药师?E-mail:312740711@qq.com